CBX2 (NM_032647) Human Tagged ORF Clone

CAT#: RC216313

CBX2 (Myc-DDK-tagged)-Human chromobox homolog 2 (CBX2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403188) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216313 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBX2
• Synonyms: CDCA6; M33; SRXY5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216313 representing NM_032647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCTGAGCAGCGTGGGCGAGCAGGTCTTCGCCGCCGAGTGCATCCTGAGCAAGCGGCTCCGCA
  AGGGCAAGCTGGAGTACCTGGTCAAGTGGCGCGGCTGGTCCTCCAAACATAACAGCTGGGAGCCGGAGGA
  GAACATCCTGGACCCGAGGCTGCTCCTGGCCTTCCAGAAGAAGGAACATGAGAAGGAGGTGCAGAACCGG
  AAGAGAGGCAAGAGGCCGAGAGGCCGGCCAAGGAAGCTCACTGCCATGTCCTCCTGCAGCCGGCGCTCCA
  AGCTCAAGGTGGGTGGCTGCGCTGGGTATGCTGACCCCACCTCCCAGCACCCCCTTGGCGTAGGGGGCAG
  GCAGAGGGAGGGTTTGGGGCCCTCAGGAAGGGGGTGGCACTTCTGCCAACAGTCTGTCCCTCTACTCGGA
  AAACAGGAGCCCCCTTTCTTCCTGTCTCTCAGCTTCTGCTGCCAGGGGCCCCAGCCGGCTGAGAGTTCCT
  CCCCGCCCCTCCCAGGGGCTTCCTGCTTCAGCCTGTCCTGCACGCCTCTCTGCTGGGTGGCAGGGTCAAA
  CTGCTGCAGACAAGCACTCTTCCCTCCCAGGGGGTCCTTGGGGGACGGAAAGGAACAGGAAGCATGCGTA
  CAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216313 representing NM_032647
  Red=Cloning site Green=Tags(s)
  
  MEELSSVGEQVFAAECILSKRLRKGKLEYLVKWRGWSSKHNSWEPEENILDPRLLLAFQKKEHEKEVQNR
  KRGKRPRGRPRKLTAMSSCSRRSKLKVGGCAGYADPTSQHPLGVGGRQREGLGPSGRGWHFCQQSVPLLG
  KQEPPFFLSLSFCCQGPQPAESSSPPLPGASCFSLSCTPLCWVAGSNCCRQALFPPRGSLGDGKEQEACV
  Q
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032647
• ORF Size: 633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032647.3
• RefSeq Size: 1061 bp
• RefSeq ORF: 636 bp
• Locus ID: 84733
• UniProt ID: Q14781
• Cytogenetics: 17q25.3
• Protein Families: Transcription Factors
• MW: 23.1 kDa
• Gene Summary: This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in mice results in male-to-female gonadal sex reversal. Mutations in this gene are also associated with gonadal dysgenesis in humans. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2010]