Adenylate Kinase 1 (AK1) (NM_000476) Human Tagged ORF Clone

CAT#: RC215130

AK1 (Myc-DDK-tagged)-Human adenylate kinase 1 (AK1)

Product Images

Circular map for RC215130

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AK1 (Cat# RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1(Cat# TA500321). Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400171) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215130 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AK1 protein (Cat# TP315130). The protein was produced from HEK293T cells transfected with AK1 cDNA clone (Cat# RC215130) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adenylate Kinase 1
• Synonyms: HTL-S-58j
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215130 representing NM_000476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCC
  AGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGGTCCGAGGT
  CAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGGCAGCTGGTTCCACTGGAG
  ACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATG
  GCTACCCGCGGGAGGTGCAGCAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTA
  TGTGGACGCAGGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
  GACAATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTCATCGCCTTCT
  ATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCCCAGGTCTG
  CACCCACCTGGACGCCCTAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215130 representing NM_000476
  Red=Cloning site Green=Tags(s)
  
  MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLE
  TVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVD
  DNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000476
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000476.2
• RefSeq Size: 2271 bp
• RefSeq ORF: 585 bp
• Locus ID: 203
• UniProt ID: P00568
• Cytogenetics: 9q34.11
• Domains: ADK
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 21.5 kDa
• Gene Summary: This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]