SETD3 (NM_032233) Human Tagged ORF Clone

CAT#: RC214566

SETD3 (Myc-DDK-tagged)-Human SET domain containing 3 (SETD3), transcript variant 1

Product Images

Circular map for RC214566

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  Western blot validation of overexpression lysate (Cat# LY410259) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214566 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SETD3 protein (Cat# TP314566). The protein was produced from HEK293T cells transfected with SETD3 cDNA clone (Cat# RC214566) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SETD3
• Synonyms: C14orf154; hSETD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214566 representing NM_032233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAAGAAGAGTCGAGTAAAAACTCAGAAATCTGGCACTGGTGCTACAGCAACTGTGTCACCAAAGG
  AAATCTTGAACCTGACCAGTGAGCTGCTGCAGAAATGCAGCAGTCCGGCGCCTGGCCCAGGAAAAGAGTG
  GGAAGAGTATGTGCAGATCCGGACTCTGGTTGAGAAAATACGGAAAAAGCAAAAAGGTCTGTCCGTTACT
  TTTGATGGAAAAAGAGAAGATTACTTTCCTGATCTAATGAAATGGGCCTCTGAAAATGGGGCTTCTGTCG
  AGGGTTTTGAAATGGTTAACTTCAAAGAAGAGGGCTTTGGTTTGAGAGCAACAAGAGATATCAAGGCAGA
  AGAATTGTTTTTATGGGTTCCACGAAAATTGCTAATGACTGTTGAATCTGCTAAAAATTCAGTGTTGGGG
  CCCTTATATTCTCAAGACCGAATCCTTCAAGCCATGGGAAACATCGCACTGGCCTTTCATTTGCTGTGTG
  AGCGAGCCAGCCCTAACTCCTTCTGGCAGCCCTATATTCAAACCCTCCCCAGTGAATATGACACTCCTCT
  CTACTTTGAAGAAGATGAAGTTCGGTATCTTCAGTCCACACAAGCTATACATGATGTCTTCAGCCAGTAT
  AAAAACACAGCTCGACAGTACGCCTACTTCTATAAAGTCATCCAGACCCATCCTCATGCCAACAAACTAC
  CCTTGAAGGATTCTTTCACTTACGAGGACTACAGGTGGGCAGTCTCTTCTGTTATGACGAGGCAAAACCA
  AATTCCCACAGAGGATGGTTCCCGCGTGACCCTGGCTCTGATTCCTTTATGGGATATGTGTAACCACACC
  AACGGCCTGATCACTACTGGTTACAACCTGGAAGATGACCGCTGTGAGTGTGTGGCTCTGCAGGATTTTC
  GGGCTGGAGAGCAGATTTACATTTTTTATGGCACTCGATCCAACGCAGAGTTTGTGATCCACAGTGGTTT
  TTTCTTTGACAATAACTCACACGACAGAGTGAAAATAAAGCTTGGAGTGAGTAAAAGTGACAGACTCTAC
  GCCATGAAGGCCGAGGTCTTGGCTCGTGCCGGCATCCCCACTTCCAGTGTTTTTGCATTGCATTTTACCG
  AGCCGCCCATCTCTGCTCAGCTTTTGGCTTTTCTCCGAGTATTCTGTATGACTGAAGAAGAACTGAAAGA
  ACACTTGCTGGGAGACAGCGCTATTGATAGAATCTTCACCTTGGGGAACTCGGAATTTCCTGTTAGCTGG
  GACAACGAGGTCAAACTTTGGACATTTCTTGAAGATAGAGCCTCACTTCTTTTAAAAACATATAAAACAA
  CTATTGAGGAAGATAAATCCGTCTTGAAAAACCACGATCTTTCTGTTCGTGCAAAAATGGCCATCAAATT
  GCGCTTAGGTGAGAAAGAGATTTTGGAAAAAGCAGTAAAGAGTGCAGCTGTCAACCGGGAATACTATCGC
  CAACAGATGGAGGAAAAGGCTCCGCTTCCCAAATATGAAGAGAGTAACCTTGGGCTGTTGGAGAGCAGCG
  TGGGGGACTCGAGGCTCCCCCTGGTCTTGAGAAACCTCGAGGAGGAGGCTGGAGTGCAGGATGCCTTGAA
  CATCAGAGAGGCAATCAGCAAAGCAAAGGCCACAGAAAACGGGCTTGTAAACGGTGAAAACTCTATCCCT
  AATGGGACCAGGTCCGAAAATGAAAGTCTCAATCAAGAAAGTAAAAGAGCAGTTGAAGACGCCAAAGGAT
  CTTCTTCAGACAGCACTGCTGGAGTTAAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214566 representing NM_032233
  Red=Cloning site Green=Tags(s)
  
  MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVT
  FDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLG
  PLYSQDRILQAMGNIALAFHLLCERASPNSFWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQY
  KNTARQYAYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRVTLALIPLWDMCNHT
  NGLITTGYNLEDDRCECVALQDFRAGEQIYIFYGTRSNAEFVIHSGFFFDNNSHDRVKIKLGVSKSDRLY
  AMKAEVLARAGIPTSSVFALHFTEPPISAQLLAFLRVFCMTEEELKEHLLGDSAIDRIFTLGNSEFPVSW
  DNEVKLWTFLEDRASLLLKTYKTTIEEDKSVLKNHDLSVRAKMAIKLRLGEKEILEKAVKSAAVNREYYR
  QQMEEKAPLPKYEESNLGLLESSVGDSRLPLVLRNLEEEAGVQDALNIREAISKAKATENGLVNGENSIP
  NGTRSENESLNQESKRAVEDAKGSSSDSTAGVKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032233
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032233.3
• RefSeq Size: 2905 bp
• RefSeq ORF: 1785 bp
• Locus ID: 84193
• UniProt ID: Q86TU7
• Cytogenetics: 14q32.2
• Domains: SET
• MW: 67.1 kDa
• Gene Summary: Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73' (PubMed:30526847, PubMed:30626964, PubMed:30785395). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed:30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed:30626964, PubMed:30785395).[UniProtKB/Swiss-Prot Function]