SETD3 (NM_032233) Human Tagged ORF Clone

CAT#: RC214566

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SETD3 (Myc-DDK-tagged)-Human SET domain containing 3 (SETD3), transcript variant 1



Reconstitution Protocol

USD 553.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SETD3
Synonyms C14orf154; hSETD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214566 representing NM_032233
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTAAGAAGAGTCGAGTAAAAACTCAGAAATCTGGCACTGGTGCTACAGCAACTGTGTCACCAAAGG
AAATCTTGAACCTGACCAGTGAGCTGCTGCAGAAATGCAGCAGTCCGGCGCCTGGCCCAGGAAAAGAGTG
GGAAGAGTATGTGCAGATCCGGACTCTGGTTGAGAAAATACGGAAAAAGCAAAAAGGTCTGTCCGTTACT
TTTGATGGAAAAAGAGAAGATTACTTTCCTGATCTAATGAAATGGGCCTCTGAAAATGGGGCTTCTGTCG
AGGGTTTTGAAATGGTTAACTTCAAAGAAGAGGGCTTTGGTTTGAGAGCAACAAGAGATATCAAGGCAGA
AGAATTGTTTTTATGGGTTCCACGAAAATTGCTAATGACTGTTGAATCTGCTAAAAATTCAGTGTTGGGG
CCCTTATATTCTCAAGACCGAATCCTTCAAGCCATGGGAAACATCGCACTGGCCTTTCATTTGCTGTGTG
AGCGAGCCAGCCCTAACTCCTTCTGGCAGCCCTATATTCAAACCCTCCCCAGTGAATATGACACTCCTCT
CTACTTTGAAGAAGATGAAGTTCGGTATCTTCAGTCCACACAAGCTATACATGATGTCTTCAGCCAGTAT
AAAAACACAGCTCGACAGTACGCCTACTTCTATAAAGTCATCCAGACCCATCCTCATGCCAACAAACTAC
CCTTGAAGGATTCTTTCACTTACGAGGACTACAGGTGGGCAGTCTCTTCTGTTATGACGAGGCAAAACCA
AATTCCCACAGAGGATGGTTCCCGCGTGACCCTGGCTCTGATTCCTTTATGGGATATGTGTAACCACACC
AACGGCCTGATCACTACTGGTTACAACCTGGAAGATGACCGCTGTGAGTGTGTGGCTCTGCAGGATTTTC
GGGCTGGAGAGCAGATTTACATTTTTTATGGCACTCGATCCAACGCAGAGTTTGTGATCCACAGTGGTTT
TTTCTTTGACAATAACTCACACGACAGAGTGAAAATAAAGCTTGGAGTGAGTAAAAGTGACAGACTCTAC
GCCATGAAGGCCGAGGTCTTGGCTCGTGCCGGCATCCCCACTTCCAGTGTTTTTGCATTGCATTTTACCG
AGCCGCCCATCTCTGCTCAGCTTTTGGCTTTTCTCCGAGTATTCTGTATGACTGAAGAAGAACTGAAAGA
ACACTTGCTGGGAGACAGCGCTATTGATAGAATCTTCACCTTGGGGAACTCGGAATTTCCTGTTAGCTGG
GACAACGAGGTCAAACTTTGGACATTTCTTGAAGATAGAGCCTCACTTCTTTTAAAAACATATAAAACAA
CTATTGAGGAAGATAAATCCGTCTTGAAAAACCACGATCTTTCTGTTCGTGCAAAAATGGCCATCAAATT
GCGCTTAGGTGAGAAAGAGATTTTGGAAAAAGCAGTAAAGAGTGCAGCTGTCAACCGGGAATACTATCGC
CAACAGATGGAGGAAAAGGCTCCGCTTCCCAAATATGAAGAGAGTAACCTTGGGCTGTTGGAGAGCAGCG
TGGGGGACTCGAGGCTCCCCCTGGTCTTGAGAAACCTCGAGGAGGAGGCTGGAGTGCAGGATGCCTTGAA
CATCAGAGAGGCAATCAGCAAAGCAAAGGCCACAGAAAACGGGCTTGTAAACGGTGAAAACTCTATCCCT
AATGGGACCAGGTCCGAAAATGAAAGTCTCAATCAAGAAAGTAAAAGAGCAGTTGAAGACGCCAAAGGAT
CTTCTTCAGACAGCACTGCTGGAGTTAAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214566 representing NM_032233
Red=Cloning site Green=Tags(s)

MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVT
FDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLG
PLYSQDRILQAMGNIALAFHLLCERASPNSFWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQY
KNTARQYAYFYKVIQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRVTLALIPLWDMCNHT
NGLITTGYNLEDDRCECVALQDFRAGEQIYIFYGTRSNAEFVIHSGFFFDNNSHDRVKIKLGVSKSDRLY
AMKAEVLARAGIPTSSVFALHFTEPPISAQLLAFLRVFCMTEEELKEHLLGDSAIDRIFTLGNSEFPVSW
DNEVKLWTFLEDRASLLLKTYKTTIEEDKSVLKNHDLSVRAKMAIKLRLGEKEILEKAVKSAAVNREYYR
QQMEEKAPLPKYEESNLGLLESSVGDSRLPLVLRNLEEEAGVQDALNIREAISKAKATENGLVNGENSIP
NGTRSENESLNQESKRAVEDAKGSSSDSTAGVKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032233
ORF Size 1782 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032233.3
RefSeq Size 2905 bp
RefSeq ORF 1785 bp
Locus ID 84193
UniProt ID Q86TU7
Cytogenetics 14q32.2
Domains SET
MW 67.1 kDa
Gene Summary Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73' (PubMed:30526847, PubMed:30626964, PubMed:30785395). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed:30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed:30626964, PubMed:30785395).[UniProtKB/Swiss-Prot Function]
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