Semaphorin 3A (SEMA3A) (NM_006080) Human Tagged ORF Clone

CAT#: RC213681

SEMA3A (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (SEMA3A)

Product Images

Circular map for RC213681

• 

  Western blot validation of overexpression lysate (Cat# LY416877) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213681 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Semaphorin 3A
• Synonyms: coll-1; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SEMAL; SemD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213681 representing NM_006080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGTTAACTAGGATTGTCTGTCTTTTCTGGGGAGTATTACTTACAGCAAGAGCAAACTATCAGA
  ATGGGAAGAACAATGTGCCAAGGCTGAAATTATCCTACAAAGAAATGTTGGAATCCAACAATGTGATCAC
  TTTCAATGGCTTGGCCAACAGCTCCAGTTATCATACCTTCCTTTTGGATGAGGAACGGAGTAGGCTGTAT
  GTTGGAGCAAAGGATCACATATTTTCATTCGACCTGGTTAATATCAAGGATTTTCAAAAGATTGTGTGGC
  CAGTATCTTACACCAGAAGAGATGAATGCAAGTGGGCTGGAAAAGACATCCTGAAAGAATGTGCTAATTT
  CATCAAGGTACTTAAGGCATATAATCAGACTCACTTGTACGCCTGTGGAACGGGGGCTTTTCATCCAATT
  TGCACCTACATTGAAATTGGACATCATCCTGAGGACAATATTTTTAAGCTGGAGAACTCACATTTTGAAA
  ACGGCCGTGGGAAGAGTCCATATGACCCTAAGCTGCTGACAGCATCCCTTTTAATAGATGGAGAATTATA
  CTCTGGAACTGCAGCTGATTTTATGGGGCGAGACTTTGCTATCTTCCGAACTCTTGGGCACCACCACCCA
  ATCAGGACAGAGCAGCATGATTCCAGGTGGCTCAATGATCCAAAGTTCATTAGTGCCCACCTCATCTCAG
  AGAGTGACAATCCTGAAGATGACAAAGTATACTTTTTCTTCCGTGAAAATGCAATAGATGGAGAACACTC
  TGGAAAAGCTACTCACGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGAGGGCACAGAAGTCTGGTG
  AATAAATGGACAACATTCCTCAAAGCTCGTCTGATTTGCTCAGTGCCAGGTCCAAATGGCATTGACACTC
  ATTTTGATGAACTGCAGGATGTATTCCTAATGAACTTTAAAGATCCTAAAAATCCAGTTGTATATGGAGT
  GTTTACGACTTCCAGTAACATTTTCAAGGGATCAGCCGTGTGTATGTATAGCATGAGTGATGTGAGAAGG
  GTGTTCCTTGGTCCATATGCCCACAGGGATGGACCCAACTATCAATGGGTGCCTTATCAAGGAAGAGTCC
  CCTATCCACGGCCAGGAACTTGTCCCAGCAAAACATTTGGTGGTTTTGACTCTACAAAGGACCTTCCTGA
  TGATGTTATAACCTTTGCAAGAAGTCATCCAGCCATGTACAATCCAGTGTTTCCTATGAACAATCGCCCA
  ATAGTGATCAAAACGGATGTAAATTATCAATTTACACAAATTGTCGTAGACCGAGTGGATGCAGAAGATG
  GACAGTATGATGTTATGTTTATCGGAACAGATGTTGGGACCGTTCTTAAAGTAGTTTCAATTCCTAAGGA
  GACTTGGTATGATTTAGAAGAGGTTCTGCTGGAAGAAATGACAGTTTTTCGGGAACCGACTGCTATTTCA
  GCAATGGAGCTTTCCACTAAGCAGCAACAACTATATATTGGTTCAACGGCTGGGGTTGCCCAGCTCCCTT
  TACACCGGTGTGATATTTACGGGAAAGCGTGTGCTGAGTGTTGCCTCGCCCGAGACCCTTACTGTGCTTG
  GGATGGTTCTGCATGTTCTCGCTATTTTCCCACTGCAAAGAGACGCACAAGACGACAAGATATAAGAAAT
  GGAGACCCACTGACTCACTGTTCAGACTTACACCATGATAATCACCATGGCCACAGCCCTGAAGAGAGAA
  TCATCTATGGTGTAGAGAATAGTAGCACATTTTTGGAATGCAGTCCGAAGTCGCAGAGAGCGCTGGTCTA
  TTGGCAATTCCAGAGGCGAAATGAAGAGCGAAAAGAAGAGATCAGAGTGGATGATCATATCATCAGGACA
  GATCAAGGCCTTCTGCTACGTAGTCTACAACAGAAGGATTCAGGCAATTACCTCTGCCATGCGGTGGAAC
  ATGGGTTCATACAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAGCATTTGGAAGAACTTCT
  TCATAAAGATGATGATGGAGATGGCTCTAAGACCAAAGAAATGTCCAATAGCATGACACCTAGCCAGAAG
  GTCTGGTACAGAGACTTCATGCAGCTCATCAACCACCCCAATCTCAACACGATGGATGAGTTCTGTGAAC
  AAGTTTGGAAAAGGGACCGAAAACAACGTCGGCAAAGGCCAGGACATACCCCAGGGAACAGTAACAAATG
  GAAGCACTTACAAGAAAATAAGAAAGGTAGAAACAGGAGGACCCACGAATTTGAGAGGGCACCCAGGAGT
  GTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213681 representing NM_006080
  Red=Cloning site Green=Tags(s)
  
  MGWLTRIVCLFWGVLLTARANYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY
  VGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPI
  CTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP
  IRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV
  NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRR
  VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRP
  IVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAIS
  AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRRTRRQDIRN
  GDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRT
  DQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQK
  VWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRS
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006080
• ORF Size: 2313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006080.1, NP_006071.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 2316 bp
• Locus ID: 10371
• UniProt ID: Q14563
• Cytogenetics: 7q21.11
• Domains: Sema, ig, IG, PSI
• Protein Families: Secreted Protein
• Protein Pathways: Axon guidance
• MW: 88.89 kDa
• Gene Summary: This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]