iNOS (NOS2) (NM_000625) Human Tagged ORF Clone

CAT#: RC211819

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  • TrueORF®

NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2)



  "NM_000625" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,371.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NOS2
Synonyms HEP-NOS; INOS; NOS; NOS2A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211819 representing NM_000625
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATGGGGAAAAAGACA
TCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGACACAGGATGACCTTCAGTATCA
CAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCCTCGTGGAGACGGGAAAGAAGTCTCCAGAATCT
CTGGTCAAGCTGGATGCAACCCCATTGTCCTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGA
TGACTTTCCAAGACACACTTCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGG
GTCCATTATGACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACCCCTCCAGATGAGCTTCTA
CCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAGAGGAACATCTGG
CCAGGGTGGAAGCGGTAACAAAGGAGATAGAAACAACAGGAACCTACCAACTGACGGGAGATGAGCTCAT
CTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCTGCATTGGGAGGATCCAGTGGTCCAACCTGCAG
GTCTTCGATGCCCGCAGCTGTTCCACTGCCCGGGAAATGTTTGAACACATCTGCAGACACGTGCGTTACT
CCACCAACAATGGCAACATCAGGTCGGCCATCACCGTGTTCCCCCAGCGGAGTGATGGCAAGCACGACTT
CCGGGTGTGGAATGCTCAGCTCATCCGCTATGCTGGCTACCAGATGCCAGATGGCAGCATCAGAGGGGAC
CCTGCCAACGTGGAATTCACTCAGCTGTGCATCGACCTGGGCTGGAAGCCCAAGTACGGCCGCTTCGATG
TGGTCCCCCTGGTCCTGCAGGCCAATGGCCGTGACCCTGAGCTCTTCGAAATCCCACCTGACCTTGTGCT
TGAGGTGGCCATGGAACATCCCAAATACGAGTGGTTTCGGGAACTGGAGCTAAAGTGGTACGCCCTGCCT
GCAGTGGCCAACATGCTGCTTGAGGTGGGCGGCCTGGAGTTCCCAGGGTGCCCCTTCAATGGCTGGTACA
TGGGCACAGAGATCGGAGTCCGGGACTTCTGTGACGTCCAGCGCTACAACATCCTGGAGGAAGTGGGCAG
GAGAATGGGCCTGGAAACGCACAAGCTGGCCTCGCTCTGGAAAGACCAGGCTGTCGTTGAGATCAACATT
GCTGTGCTCCATAGTTTCCAGAAGCAGAATGTGACCATCATGGACCACCACTCGGCTGCAGAATCCTTCA
TGAAGTACATGCAGAATGAATACCGGTCCCGTGGGGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCC
CATGTCTGGGAGCATCACCCCCGTGTTTCACCAGGAGATGCTGAACTACGTCCTGTCCCCTTTCTACTAC
TATCAGGTAGAGGCCTGGAAAACCCATGTCTGGCAGGACGAGAAGCGGAGACCCAAGAGAAGAGAGATTC
CATTGAAAGTCTTGGTCAAAGCTGTGCTCTTTGCCTGTATGCTGATGCGCAAGACAATGGCGTCCCGAGT
CAGAGTCACCATCCTCTTTGCGACAGAGACAGGAAAATCAGAGGCGCTGGCCTGGGACCTGGGGGCCTTA
TTCAGCTGTGCCTTCAACCCCAAGGTTGTCTGCATGGATAAGTACAGGCTGAGCTGCCTGGAGGAGGAAC
GGCTGCTGTTGGTGGTGACCAGTACGTTTGGCAATGGAGACTGCCCTGGCAATGGAGAGAAACTGAAGAA
ATCGCTCTTCATGCTGAAAGAGCTCAACAACAAATTCAGGTACGCTGTGTTTGGCCTCGGCTCCAGCATG
TACCCTCGGTTCTGCGCCTTTGCTCATGACATTGATCAGAAGCTGTCCCACCTGGGGGCCTCTCAGCTCA
CCCCGATGGGAGAAGGGGATGAGCTCAGTGGGCAGGAGGACGCCTTCCGCAGCTGGGCCGTGCAAACCTT
CAAGGCAGCCTGTGAGACGTTTGATGTCCGAGGCAAACAGCACATTCAGATCCCCAAGCTCTACACCTCC
AATGTGACCTGGGACCCGCACCACTACAGGCTCGTGCAGGACTCACAGCCTTTGGACCTCAGCAAAGCCC
TCAGCAGCATGCATGCCAAGAACGTGTTCACCATGAGGCTCAAATCTCGGCAGAATCTACAAAGTCCGAC
ATCCAGCCGTGCCACCATCCTGGTGGAACTCTCCTGTGAGGATGGCCAAGGCCTGAACTACCTGCCGGGG
GAGCACCTTGGGGTTTGCCCAGGCAACCAGCCGGCCCTGGTCCAAGGTATCCTGGAGCGAGTGGTGGATG
GCCCCACACCCCACCAGACAGTGCGCCTGGAGGCCCTGGATGAGAGTGGCAGCTACTGGGTCAGTGACAA
GAGGCTGCCCCCCTGCTCACTCAGCCAGGCCCTCACCTACTTCCTGGACATCACCACACCCCCAACCCAG
CTGCTGCTCCAAAAGCTGGCCCAGGTGGCCACAGAAGAGCCTGAGAGACAGAGGCTGGAGGCCCTGTGCC
AGCCCTCAGAGTACAGCAAGTGGAAGTTCACCAACAGCCCCACATTCCTGGAGGTGCTAGAGGAGTTCCC
GTCCCTGCGGGTGTCTGCTGGCTTCCTGCTTTCCCAGCTCCCCATTCTGAAGCCCAGGTTCTACTCCATC
AGCTCCTCCCGGGATCACATGCCCACAGAGATCCACCTGACTGTGGCCGTGGTCACCTACCACACCCGAG
ATGGCCAGGGTCCCCTGCACCACGGCGTCTGCAGCACATGGCTCAACAGCCTGAAGCCCCAAGACCCAGT
GCCCTGCTTTGTGCGGAATGCCAGCGGCTTCCACCTCCCCGAGGATCCCTCCCATCCTTGCATCCTCATC
GGGCCTGGCACAGGCATCGCGCCCTTCCGCAGTTTCTGGCAGCAACGGCTCCATGACTCCCAGCACAAGG
GAGTGCGGGGAGGCCGCATGACCTTGGTGTTTGGGTGCCGCCGCCCAGATGAGGACCACATCTACCAGGA
GGAGATGCTGGAGATGGCCCAGAAGGGGGTGCTGCATGCGGTGCACACAGCCTATTCCCGCCTGCCTGGC
AAGCCCAAGGTCTATGTTCAGGACATCCTGCGGCAGCAGCTGGCCAGCGAGGTGCTCCGTGTGCTCCACA
AGGAGCCAGGCCACCTCTATGTTTGCGGGGATGTGCGCATGGCCCGGGACGTGGCCCACACCCTGAAGCA
GCTGGTGGCTGCCAAGCTGAAATTGAATGAGGAGCAGGTCGAGGACTATTTCTTTCAGCTCAAGAGCCAG
AAGCGCTATCACGAAGATATCTTTGGTGCTGTATTTCCTTACGAGGCGAAGAAGGACAGGGTGGCGGTGC
AGCCCAGCAGCCTGGAGATGTCAGCGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211819 representing NM_000625
Red=Cloning site Green=Tags(s)

MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPES
LVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELL
PQAIEFVNKYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQ
VFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGD
PANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALP
AVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINI
AVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYY
YQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGAL
FSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSM
YPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTS
NVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPG
EHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQ
LLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSI
SSSRDHMPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILI
GPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPG
KPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQ
KRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000625
ORF Size 3459 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000625.4
RefSeq Size 4221 bp
RefSeq ORF 3462 bp
Locus ID 4843
UniProt ID P35228
Cytogenetics 17q11.2
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
MW 130.9 kDa
Gene Summary Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

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