PODXL (NM_005397) Human Tagged ORF Clone

CAT#: RC210816

PODXL (Myc-DDK-tagged)-Human podocalyxin-like (PODXL), transcript variant 2

Product Images

Circular map for RC210816

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  Western blot validation of overexpression lysate (Cat# LY401657) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210816 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PODXL protein (Cat# TP310816). The protein was produced from HEK293T cells transfected with PODXL cDNA clone (Cat# RC210816) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PODXL
• Synonyms: gp135; Gp200; PC; PCLP; PCLP-1; PDX; PODXL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210816 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGC
  CGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACC
  GACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAG
  GCCAACGAAATCTTGGCCTCAGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAA
  CCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTAC
  TACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA
  GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCC
  ACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACT
  TAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATG
  AAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCA
  CCCTACCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCCAGTCAGATGCCAGCCAGCTCTACGGC
  CCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGGCATTGAGAACACCTACCCTGCCAGAG
  ACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGG
  CTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCT
  GAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATATGCCGA
  GCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCATCTGTTCCAGGAAGTC
  AGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCTGCCAAGGATGTGTACGAGCGGCTGAA
  GGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAG
  GAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCG
  TGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGA
  GCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCTGAGATG
  CAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTCCCTCTGGACAACCTGA
  CCAAGGACGACCTGGATGAGGAGGAAGACACACACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210816 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRCALALSALLLLLSTPPLLPSSPSPSPSPSQNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSK
  ANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTAT
  AKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLM
  KISSSSSTVAIPGYTFTSPGMTTTLPSSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTLPE
  TMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICR
  AVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPE
  EAEDRFSMPLIITIVCMASFLLLVAALYGCCHQRLSQRKDQQRLTEELQTVENGYHDNPTLEVMETSSEM
  QEKKVVSLNGELGDSWIVPLDNLTKDDLDEEEDTHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005397
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005397.4
• RefSeq Size: 5911 bp
• RefSeq ORF: 1581 bp
• Locus ID: 5420
• UniProt ID: O00592
• Cytogenetics: 7q32.3
• Protein Families: Transmembrane
• MW: 55.4 kDa
• Gene Summary: This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]