PODXL (NM_005397) Human Tagged ORF Clone

CAT#: RC210816

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PODXL (Myc-DDK-tagged)-Human podocalyxin-like (PODXL), transcript variant 2



  "NM_005397" in other vectors (6)

Reconstitution Protocol

USD 490.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PODXL
Synonyms gp135; Gp200; PC; PCLP; PCLP-1; PDX; PODXL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210816 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGC
CGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACC
GACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAG
GCCAACGAAATCTTGGCCTCAGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAA
CCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTAC
TACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA
GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCC
ACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACT
TAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATG
AAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCA
CCCTACCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCCAGTCAGATGCCAGCCAGCTCTACGGC
CCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGGCATTGAGAACACCTACCCTGCCAGAG
ACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGG
CTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCT
GAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATATGCCGA
GCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCATCTGTTCCAGGAAGTC
AGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCTGCCAAGGATGTGTACGAGCGGCTGAA
GGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAG
GAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCG
TGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGA
GCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCTGAGATG
CAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTCCCTCTGGACAACCTGA
CCAAGGACGACCTGGATGAGGAGGAAGACACACACCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210816 protein sequence
Red=Cloning site Green=Tags(s)

MRCALALSALLLLLSTPPLLPSSPSPSPSPSQNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSK
ANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTAT
AKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLM
KISSSSSTVAIPGYTFTSPGMTTTLPSSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTLPE
TMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICR
AVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPE
EAEDRFSMPLIITIVCMASFLLLVAALYGCCHQRLSQRKDQQRLTEELQTVENGYHDNPTLEVMETSSEM
QEKKVVSLNGELGDSWIVPLDNLTKDDLDEEEDTHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005397
ORF Size 1578 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005397.4
RefSeq Size 5911 bp
RefSeq ORF 1581 bp
Locus ID 5420
UniProt ID O00592
Cytogenetics 7q32.3
Protein Families Transmembrane
MW 55.4 kDa
Gene Summary This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

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