HLAB (HLA-B) (NM_005514) Human Tagged Lenti ORF Clone
CAT#: RC210631L3
- LentiORF®
Lenti ORF clone of Human major histocompatibility complex, class I, B (HLA-B), Myc-DDK-tagged
"NM_005514" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HLAB |
Synonyms | AS; B-4901; HLAB |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC210631).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005514 |
ORF Size | 1086 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005514.5 |
RefSeq Size | 1578 bp |
RefSeq ORF | 1089 bp |
Locus ID | 3106 |
UniProt ID | P01889 |
Cytogenetics | 6p21.33 |
Domains | MHC_I, ig, IGc1 |
Protein Families | Transmembrane |
Protein Pathways | Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis |
MW | 40.3 kDa |
Gene Summary | HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210631 | HLA (Myc-DDK-tagged)-Human major histocompatibility complex, class I, B (HLA-B) |
USD 457.00 |
|
RC210631L1 | Lenti ORF clone of Human major histocompatibility complex, class I, B (HLA-B), Myc-DDK-tagged |
USD 757.00 |
|
RC210631L2 | Lenti ORF clone of Human major histocompatibility complex, class I, B (HLA-B), mGFP tagged |
USD 757.00 |
|
RC210631L4 | Lenti ORF clone of Human major histocompatibility complex, class I, B (HLA-B), mGFP tagged |
USD 757.00 |
|
RG210631 | HLA (tGFP-tagged) - Human major histocompatibility complex, class I, B (HLA-B) |
USD 657.00 |
|
SC124484 | HLA (untagged)-Human major histocompatibility complex, class I, B (HLA-B) |
USD 457.00 |
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