RAB21 (NM_014999) Human Tagged ORF Clone
RAB21 (Myc-DDK-tagged)-Human RAB21, member RAS oncogene family (RAB21)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC210510 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210510 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||675 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2627 bp|
|RefSeq ORF||678 bp|
|Domains||ras, RAN, RAS, RHO, RAB|
|Protein Families||Druggable Genome|
|Gene Summary||This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015]|
|cDNA Clone Resources|
|RC210510L1||Lenti ORF clone of Human RAB21, member RAS oncogene family (RAB21), Myc-DDK-tagged||
|RC210510L2||Lenti ORF clone of Human RAB21, member RAS oncogene family (RAB21), mGFP tagged||
|RC210510L3||Lenti ORF clone of Human RAB21, member RAS oncogene family (RAB21), Myc-DDK-tagged||
|RC210510L4||Lenti ORF clone of Human RAB21, member RAS oncogene family (RAB21), mGFP tagged||
|RG210510||RAB21 (tGFP-tagged) - Human RAB21, member RAS oncogene family (RAB21)||
|SC114700||RAB21 (untagged)-Human RAB21, member RAS oncogene family (RAB21)||