BCL10 (NM_003921) Human Tagged ORF Clone
BCL10 (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 10 (BCL10)
|Product Name||BCL10 (NM_003921) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||c-E10; CARMEN; CIPER; CLAP; IMD37; mE10|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC208752 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC208752 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||699 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_003921.1, NM_003921.2, NM_003921.3, NM_003921.4, NP_003912.1|
|RefSeq Size||3118 bp|
|RefSeq ORF||702 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||B cell receptor signaling pathway, T cell receptor signaling pathway|
|Gene Summary||This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]|
|The use of this cDNA Clones has been cited in the following citations:|
Impaired RASGRF1/ERK-mediated GM-CSF response characterizes CARD9 deficiency in French-Canadians
,Gavino, C;Hamel, N;Zeng, JB;Legault, C;Guiot, MC;Chankowsky, J;Lejtenyi, D;Lemire, M;Alarie, I;Dufresne, S;Boursiquot, JN;McIntosh, F;Langelier, M;Behr, MA;Sheppard, DC;Foulkes, WD;Vinh, DC;,
J. Allergy Clin. Immunol. Oct 2015
,PubMed ID 26521038
|cDNA Clone Resources|
|SC117685||BCL10 (untagged)-Human B-cell CLL/lymphoma 10 (BCL10)||
|RG208752||BCL10 (GFP-tagged) - Human B-cell CLL/lymphoma 10 (BCL10)||
|RC208752L1||Lenti ORF clone of Human B-cell CLL/lymphoma 10 (BCL10), Myc-DDK-tagged||
|RC208752L2||Lenti ORF clone of Human B-cell CLL/lymphoma 10 (BCL10), mGFP tagged||
|RC208752L3||Lenti ORF clone of Human B-cell CLL/lymphoma 10 (BCL10), Myc-DDK-tagged||
|RC208752L4||Lenti ORF clone of Human B-cell CLL/lymphoma 10 (BCL10), mGFP tagged||