SGPL1 (NM_003901) Human Tagged ORF Clone

CAT#: RC208705

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SGPL1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1)



  "NM_003901" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SGPL1
Synonyms NPHS14; S1PL; SPL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208705 representing NM_003901
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTAGCACAGACCTTCTGATGTTGAAGGCCTTTGAGCCCTACTTAGAGATTTTGGAAGTATACTCCA
CAAAAGCCAAGAATTATGTAAATGGACATTGCACCAAGTATGAGCCCTGGCAGCTAATTGCATGGAGTGT
CGTGTGGACCCTGCTGATAGTCTGGGGATATGAGTTTGTCTTCCAGCCAGAGAGTTTATGGTCAAGGTTT
AAAAAGAAATGTTTTAAGCTCACCAGGAAGATGCCCATTATTGGTCGTAAGATTCAAGACAAGTTGAACA
AGACCAAGGATGATATTAGCAAGAACATGTCATTCCTGAAAGTGGACAAAGAGTATGTGAAAGCTTTACC
CTCCCAGGGTCTGAGCTCATCTGCTGTTTTGGAGAAACTTAAGGAGTACAGCTCTATGGACGCCTTCTGG
CAAGAGGGGAGAGCCTCTGGAACAGTGTACAGTGGGGAGGAGAAGCTCACTGAGCTCCTTGTGAAGGCTT
ATGGAGATTTTGCATGGAGTAACCCCCTGCATCCAGATATCTTCCCAGGACTACGCAAGATAGAGGCAGA
AATTGTGAGGATAGCTTGTTCCCTGTTCAATGGGGGACCAGATTCGTGTGGATGTGTGACTTCTGGGGGA
ACAGAAAGCATACTGATGGCCTGCAAAGCATATCGGGATCTGGCCTTTGAGAAGGGGATCAAAACTCCAG
AAATTGTGGCTCCCCAAAGTGCCCATGCTGCATTTAACAAAGCAGCCAGTTACTTTGGGATGAAGATTGT
GCGGGTCCCATTGACGAAGATGATGGAGGTGGATGTGCGGGCAATGAGAAGAGCTATCTCCAGGAACACT
GCCATGCTCGTCTGTTCTACCCCACAGTTTCCTCATGGTGTAATAGATCCTGTCCCTGAAGTGGCCAAGC
TGGCTGTCAAATACAAAATACCCCTTCATGTCGACGCTTGTCTGGGAGGCTTCCTCATCGTCTTTATGGA
GAAAGCAGGATACCCACTGGAGCACCCATTTGATTTCCGGGTGAAAGGTGTAACCAGCATTTCAGCTGAC
ACCCATAAGTATGGCTATGCCCCAAAAGGCTCATCATTGGTGTTGTATAGTGACAAGAAGTACAGGAACT
ATCAGTTCTTCGTCGATACAGATTGGCAGGGTGGCATCTATGCTTCCCCAACCATCGCAGGCTCACGGCC
TGGTGGCATTAGCGCAGCCTGTTGGGCTGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACC
AAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTGGAAAATATCAAAGGCATCTTTGTTTTTG
GGAATCCCCAATTGTCAGTCATTGCTCTGGGATCCCGTGATTTTGACATCTACCGACTATCAAACCTGAT
GACTGCTAAGGGGTGGAACTTGAACCAGTTGCAGTTCCCACCCAGTATTCATTTCTGCATCACATTACTA
CACGCCCGGAAACGAGTAGCTATACAATTCCTAAAGGACATTCGAGAATCTGTCACTCAAATCATGAAGA
ATCCTAAAGCGAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGACAACTGTTGACAGGAATAT
GGTTGCAGAATTGTCCTCAGTCTTCTTGGACAGCTTGTACAGCACCGACACTGTCACCCAGGGCAGCCAG
ATGAATGGTTCTCCAAAACCCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208705 representing NM_003901
Red=Cloning site Green=Tags(s)

MPSTDLLMLKAFEPYLEILEVYSTKAKNYVNGHCTKYEPWQLIAWSVVWTLLIVWGYEFVFQPESLWSRF
KKKCFKLTRKMPIIGRKIQDKLNKTKDDISKNMSFLKVDKEYVKALPSQGLSSSAVLEKLKEYSSMDAFW
QEGRASGTVYSGEEKLTELLVKAYGDFAWSNPLHPDIFPGLRKIEAEIVRIACSLFNGGPDSCGCVTSGG
TESILMACKAYRDLAFEKGIKTPEIVAPQSAHAAFNKAASYFGMKIVRVPLTKMMEVDVRAMRRAISRNT
AMLVCSTPQFPHGVIDPVPEVAKLAVKYKIPLHVDACLGGFLIVFMEKAGYPLEHPFDFRVKGVTSISAD
THKYGYAPKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGYVEAT
KQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQLQFPPSIHFCITLL
HARKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQTTVDRNMVAELSSVFLDSLYSTDTVTQGSQ
MNGSPKPH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003901
ORF Size 1704 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003901.2, NP_003892.2
RefSeq Size 4701 bp
RefSeq ORF 1707 bp
Locus ID 8879
UniProt ID O95470
Cytogenetics 10q22.1
Domains pyridoxal_deC
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Sphingolipid metabolism
MW 63.3 kDa
Gene Summary Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).[UniProtKB/Swiss-Prot Function]

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