SGPL1 (NM_003901) Human Tagged ORF Clone

CAT#: RC208705

SGPL1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418357) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208705 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SGPL1 protein (Cat# TP308705). The protein was produced from HEK293T cells transfected with SGPL1 cDNA clone (Cat# RC208705) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SGPL1
• Synonyms: NPHS14; S1PL; SPL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208705 representing NM_003901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAGCACAGACCTTCTGATGTTGAAGGCCTTTGAGCCCTACTTAGAGATTTTGGAAGTATACTCCA
  CAAAAGCCAAGAATTATGTAAATGGACATTGCACCAAGTATGAGCCCTGGCAGCTAATTGCATGGAGTGT
  CGTGTGGACCCTGCTGATAGTCTGGGGATATGAGTTTGTCTTCCAGCCAGAGAGTTTATGGTCAAGGTTT
  AAAAAGAAATGTTTTAAGCTCACCAGGAAGATGCCCATTATTGGTCGTAAGATTCAAGACAAGTTGAACA
  AGACCAAGGATGATATTAGCAAGAACATGTCATTCCTGAAAGTGGACAAAGAGTATGTGAAAGCTTTACC
  CTCCCAGGGTCTGAGCTCATCTGCTGTTTTGGAGAAACTTAAGGAGTACAGCTCTATGGACGCCTTCTGG
  CAAGAGGGGAGAGCCTCTGGAACAGTGTACAGTGGGGAGGAGAAGCTCACTGAGCTCCTTGTGAAGGCTT
  ATGGAGATTTTGCATGGAGTAACCCCCTGCATCCAGATATCTTCCCAGGACTACGCAAGATAGAGGCAGA
  AATTGTGAGGATAGCTTGTTCCCTGTTCAATGGGGGACCAGATTCGTGTGGATGTGTGACTTCTGGGGGA
  ACAGAAAGCATACTGATGGCCTGCAAAGCATATCGGGATCTGGCCTTTGAGAAGGGGATCAAAACTCCAG
  AAATTGTGGCTCCCCAAAGTGCCCATGCTGCATTTAACAAAGCAGCCAGTTACTTTGGGATGAAGATTGT
  GCGGGTCCCATTGACGAAGATGATGGAGGTGGATGTGCGGGCAATGAGAAGAGCTATCTCCAGGAACACT
  GCCATGCTCGTCTGTTCTACCCCACAGTTTCCTCATGGTGTAATAGATCCTGTCCCTGAAGTGGCCAAGC
  TGGCTGTCAAATACAAAATACCCCTTCATGTCGACGCTTGTCTGGGAGGCTTCCTCATCGTCTTTATGGA
  GAAAGCAGGATACCCACTGGAGCACCCATTTGATTTCCGGGTGAAAGGTGTAACCAGCATTTCAGCTGAC
  ACCCATAAGTATGGCTATGCCCCAAAAGGCTCATCATTGGTGTTGTATAGTGACAAGAAGTACAGGAACT
  ATCAGTTCTTCGTCGATACAGATTGGCAGGGTGGCATCTATGCTTCCCCAACCATCGCAGGCTCACGGCC
  TGGTGGCATTAGCGCAGCCTGTTGGGCTGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACC
  AAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTGGAAAATATCAAAGGCATCTTTGTTTTTG
  GGAATCCCCAATTGTCAGTCATTGCTCTGGGATCCCGTGATTTTGACATCTACCGACTATCAAACCTGAT
  GACTGCTAAGGGGTGGAACTTGAACCAGTTGCAGTTCCCACCCAGTATTCATTTCTGCATCACATTACTA
  CACGCCCGGAAACGAGTAGCTATACAATTCCTAAAGGACATTCGAGAATCTGTCACTCAAATCATGAAGA
  ATCCTAAAGCGAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGACAACTGTTGACAGGAATAT
  GGTTGCAGAATTGTCCTCAGTCTTCTTGGACAGCTTGTACAGCACCGACACTGTCACCCAGGGCAGCCAG
  ATGAATGGTTCTCCAAAACCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208705 representing NM_003901
  Red=Cloning site Green=Tags(s)
  
  MPSTDLLMLKAFEPYLEILEVYSTKAKNYVNGHCTKYEPWQLIAWSVVWTLLIVWGYEFVFQPESLWSRF
  KKKCFKLTRKMPIIGRKIQDKLNKTKDDISKNMSFLKVDKEYVKALPSQGLSSSAVLEKLKEYSSMDAFW
  QEGRASGTVYSGEEKLTELLVKAYGDFAWSNPLHPDIFPGLRKIEAEIVRIACSLFNGGPDSCGCVTSGG
  TESILMACKAYRDLAFEKGIKTPEIVAPQSAHAAFNKAASYFGMKIVRVPLTKMMEVDVRAMRRAISRNT
  AMLVCSTPQFPHGVIDPVPEVAKLAVKYKIPLHVDACLGGFLIVFMEKAGYPLEHPFDFRVKGVTSISAD
  THKYGYAPKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGYVEAT
  KQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQLQFPPSIHFCITLL
  HARKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQTTVDRNMVAELSSVFLDSLYSTDTVTQGSQ
  MNGSPKPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003901
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003901.2, NP_003892.2
• RefSeq Size: 4701 bp
• RefSeq ORF: 1707 bp
• Locus ID: 8879
• UniProt ID: O95470
• Cytogenetics: 10q22.1
• Domains: pyridoxal_deC
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 63.3 kDa
• Gene Summary: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).[UniProtKB/Swiss-Prot Function]