PARG (NM_003631) Human Tagged ORF Clone

CAT#: RC208530

PARG (Myc-DDK-tagged)-Human poly (ADP-ribose) glycohydrolase (PARG)

Product Images

Circular map for RC208530

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PARG (Cat# RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG (Cat# TA808614)(1:2000). Positive lysates LY418533 (100ug) and LC418533 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418533) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208530 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARG
• Synonyms: PARG99
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208530 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCGGGCCCCGGCTGTGAACCCTGCACCAAGCGACCCCGCTGGGGCGCCGCTACAACTTCGCCGG
  CTGCTTCGGACGCCCGGAGCTTTCCCAGCAGGCAGAGGCGCGTCCTCGACCCCAAGGACGCTCACGTGCA
  GTTCAGGGTCCCACCGTCCTCGCCAGCCTGCGTCCCAGGGCGGGCGGGACAGCACAGAGGCAGCGCCACC
  TCGCTTGTTTTCAAACAAAAGACTATTACCAGTTGGATGGACACTAAAGGAATCAAGACAGCGGAATCAG
  AAAGTTTGGATAGTAAAGAAAACAACAATACAAGAATAGAATCCATGATGAGTTCTGTACAAAAAGATAA
  CTTTTACCAACATAATGTAGAAAAATTAGAAAATGTTTCTCAGCTAAGTCTTGATAAGTCACCCACTGAA
  AAAAGTACACAGTATTTGAACCAGCATCAGACTGCAGCAATGTGTAAGTGGCAAAATGAAGGGAAACACA
  CGGAGCAGCTTTTGGAAAGTGAACCTCAAACAGTAACCCTGGTACCAGAGCAGTTTAGTAATGCTAACAT
  TGATCGGTCACCTCAAAATGATGATCACAGTGACACAGATAGTGAAGAGAATAGAGACAATCAACAGTTT
  CTCACAACTGTAAAGCTTGCAAATGCAAAGCAGACTACAGAAGATGAACAGGCCAGAGAAGCCAAAAGCC
  ACCAGAAGTGCAGCAAGTCTTGCGATCCTGGGGAAGACTGTGCAAGTTGTCAGCAAGATGAGATAGATGT
  GGTGCCAGAGAGTCCATTGTCAGATGTTGGCTCTGAGGATGTTGGTACTGGGCCAAAAAATGACAACAAA
  TTGACTAGACAAGAAAGTTGCCTAGGAAATTCTCCTCCATTTGAGAAGGAAAGTGAACCCGAGTCACCGA
  TGGATGTGGATAATTCTAAAAATAGTTGTCAAGACTCAGAAGCAGATGAGGAGACAAGTCCAGGTTTTGA
  TGAACAAGAAGATGGTAGTTCCTCCCAAACAGCAAATAAACCTTCAAGGTTCCAAGCAAGAGACGCTGAC
  ATTGAATTTAGGAAACGGTACTCTACTAAGGGCGGTGAAGTTAGATTACATTTCCAATTTGAAGGAGGAG
  AGAGTCGCACTGGAATGAATGATTTAAATGCTAAACTACCTGGAAATATTTCTAGCCTGAATGTAGAATG
  CAGAAATTCTAAGCAACATGGAAAAAAGGATTCTAAAATCACAGATCATTTCATGAGACTGCCCAAAGCA
  GAGGACAGAAGAAAAGAACAGTGGGAAACCAAACATCAAAGAACAGAAAGGAAGATCCCTAAATACGTTC
  CACCTCACCTTTCTCCAGATAAGAAGTGGCTTGGAACTCCCATTGAGGAGATGAGAAGAATGCCTCGGTG
  TGGGATCCGGCTGCCTCTCTTGAGACCATCTGCCAATCACACAGTAACTATTCGGGTAGATCTTTTGCGA
  GCAGGAGAAGTTCCTAAACCTTTTCCAACACATTATAAAGATTTGTGGGATAACAAGCATGTTAAAATGC
  CTTGTTCAGAACAAAATTTGTACCCAGTGGAAGATGAGAATGGTGAGCGAACTGCGGGGAGCCGGTGGGA
  GCTCATTCAGACTGCACTTCTCAACAAATTTACACGACCCCAAAACTTGAAGGATGCTATTCTGAAATAC
  AATGTGGCATATTCTAAGAAATGGGACTTTACAGCTTTGATCGATTTCTGGGATAAGGTACTTGAAGAAG
  CAGAAGCTCAACATTTATATCAGTCCATCTTGCCTGATATGGTGAAAATTGCACTCTGTCTGCCAAATAT
  TTGCACCCAGCCAATACCACTCCTGAAACAGAAGATGAATCATTCCATCACAATGTCGCAGGAACAGATT
  GCCAGTCTTTTAGCTAATGCTTTCTTCTGCACATTTCCACGACGAAATGCTAAGATGAAATCGGAGTATT
  CTAGTTACCCAGACATTAACTTCAATCGATTGTTTGAGGGACGTTCATCAAGGAAACCGGAGAAACTTAA
  AACGCTCTTCTGCTACTTTAGAAGAGTCACAGAGAAAAAACCTACTGGGTTGGTGACATTTACAAGACAG
  AGTCTTGAAGATTTTCCAGAATGGGAAAGATGTGAAAAACCCTTGACACGATTGCATGTCACTTACGAAG
  GTACCATAGAAGAAAATGGCCAAGGCATGCTACAGGTGGATTTTGCAAATCGTTTTGTTGGAGGTGGTGT
  AACCAGTGCAGGACTTGTGCAAGAAGAAATCCGCTTTTTAATCAATCCTGAGTTGATTATTTCACGGCTC
  TTCACTGAGGTGCTGGATCACAATGAATGTCTAATTATCACAGGTACTGAGCAGTACAGTGAATACACAG
  GCTATGCTGAGACATATCGTTGGTCCCGGAGCCACGAAGATGGGAGTGAAAGGGACGACTGGCAGCGGCG
  CTGCACTGAGATCGTTGCCATCGATGCTCTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAA
  ATGAGACGCGAGCTGAACAAGGCTTACTGTGGATTTCTCCGTCCTGGAGTTTCTTCAGAGAATCTTTCTG
  CAGTGGCCACAGGAAACTGGGGCTGTGGTGCCTTTGGGGGTGATGCCAGGTTAAAAGCCTTAATACAGAT
  ATTGGCAGCTGCTGCAGCTGAGCGAGATGTGGTTTATTTCACCTTTGGGGACTCAGAATTGATGAGAGAC
  ATTTACAGCATGCACATTTTCCTTACTGAAAGGAAACTCACTGTTGGAGATGTGTATAAGCTGTTGCTAC
  GATACTACAATGAAGAATGCAGAAACTGTTCCACCCCTGGACCAGACATCAAGCTTTATCCATTCATATA
  CCATGCTGTCGAGTCCTGTGCAGAGACCGCTGACCATTCAGGGCAAAGGACAGGGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208530 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSAT
  SLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTE
  KSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQF
  LTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNK
  LTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDAD
  IEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKA
  EDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLR
  AGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKY
  NVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQI
  ASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQ
  SLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRL
  FTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEK
  MRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRD
  IYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003631
• ORF Size: 2928 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003631.5
• RefSeq Size: 4276 bp
• RefSeq ORF: 2931 bp
• Locus ID: 8505
• UniProt ID: Q86W56
• Cytogenetics: 10q11.23
• Domains: PARG
• MW: 111.1 kDa
• Gene Summary: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]