PARG (NM_003631) Human Tagged ORF Clone

CAT#: RC208530

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PARG (Myc-DDK-tagged)-Human poly (ADP-ribose) glycohydrolase (PARG)



  "NM_003631" in other vectors (7)

Reconstitution Protocol

USD 894.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Other products for "PARG"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PARG
Synonyms PARG99
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208530 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGCGGGCCCCGGCTGTGAACCCTGCACCAAGCGACCCCGCTGGGGCGCCGCTACAACTTCGCCGG
CTGCTTCGGACGCCCGGAGCTTTCCCAGCAGGCAGAGGCGCGTCCTCGACCCCAAGGACGCTCACGTGCA
GTTCAGGGTCCCACCGTCCTCGCCAGCCTGCGTCCCAGGGCGGGCGGGACAGCACAGAGGCAGCGCCACC
TCGCTTGTTTTCAAACAAAAGACTATTACCAGTTGGATGGACACTAAAGGAATCAAGACAGCGGAATCAG
AAAGTTTGGATAGTAAAGAAAACAACAATACAAGAATAGAATCCATGATGAGTTCTGTACAAAAAGATAA
CTTTTACCAACATAATGTAGAAAAATTAGAAAATGTTTCTCAGCTAAGTCTTGATAAGTCACCCACTGAA
AAAAGTACACAGTATTTGAACCAGCATCAGACTGCAGCAATGTGTAAGTGGCAAAATGAAGGGAAACACA
CGGAGCAGCTTTTGGAAAGTGAACCTCAAACAGTAACCCTGGTACCAGAGCAGTTTAGTAATGCTAACAT
TGATCGGTCACCTCAAAATGATGATCACAGTGACACAGATAGTGAAGAGAATAGAGACAATCAACAGTTT
CTCACAACTGTAAAGCTTGCAAATGCAAAGCAGACTACAGAAGATGAACAGGCCAGAGAAGCCAAAAGCC
ACCAGAAGTGCAGCAAGTCTTGCGATCCTGGGGAAGACTGTGCAAGTTGTCAGCAAGATGAGATAGATGT
GGTGCCAGAGAGTCCATTGTCAGATGTTGGCTCTGAGGATGTTGGTACTGGGCCAAAAAATGACAACAAA
TTGACTAGACAAGAAAGTTGCCTAGGAAATTCTCCTCCATTTGAGAAGGAAAGTGAACCCGAGTCACCGA
TGGATGTGGATAATTCTAAAAATAGTTGTCAAGACTCAGAAGCAGATGAGGAGACAAGTCCAGGTTTTGA
TGAACAAGAAGATGGTAGTTCCTCCCAAACAGCAAATAAACCTTCAAGGTTCCAAGCAAGAGACGCTGAC
ATTGAATTTAGGAAACGGTACTCTACTAAGGGCGGTGAAGTTAGATTACATTTCCAATTTGAAGGAGGAG
AGAGTCGCACTGGAATGAATGATTTAAATGCTAAACTACCTGGAAATATTTCTAGCCTGAATGTAGAATG
CAGAAATTCTAAGCAACATGGAAAAAAGGATTCTAAAATCACAGATCATTTCATGAGACTGCCCAAAGCA
GAGGACAGAAGAAAAGAACAGTGGGAAACCAAACATCAAAGAACAGAAAGGAAGATCCCTAAATACGTTC
CACCTCACCTTTCTCCAGATAAGAAGTGGCTTGGAACTCCCATTGAGGAGATGAGAAGAATGCCTCGGTG
TGGGATCCGGCTGCCTCTCTTGAGACCATCTGCCAATCACACAGTAACTATTCGGGTAGATCTTTTGCGA
GCAGGAGAAGTTCCTAAACCTTTTCCAACACATTATAAAGATTTGTGGGATAACAAGCATGTTAAAATGC
CTTGTTCAGAACAAAATTTGTACCCAGTGGAAGATGAGAATGGTGAGCGAACTGCGGGGAGCCGGTGGGA
GCTCATTCAGACTGCACTTCTCAACAAATTTACACGACCCCAAAACTTGAAGGATGCTATTCTGAAATAC
AATGTGGCATATTCTAAGAAATGGGACTTTACAGCTTTGATCGATTTCTGGGATAAGGTACTTGAAGAAG
CAGAAGCTCAACATTTATATCAGTCCATCTTGCCTGATATGGTGAAAATTGCACTCTGTCTGCCAAATAT
TTGCACCCAGCCAATACCACTCCTGAAACAGAAGATGAATCATTCCATCACAATGTCGCAGGAACAGATT
GCCAGTCTTTTAGCTAATGCTTTCTTCTGCACATTTCCACGACGAAATGCTAAGATGAAATCGGAGTATT
CTAGTTACCCAGACATTAACTTCAATCGATTGTTTGAGGGACGTTCATCAAGGAAACCGGAGAAACTTAA
AACGCTCTTCTGCTACTTTAGAAGAGTCACAGAGAAAAAACCTACTGGGTTGGTGACATTTACAAGACAG
AGTCTTGAAGATTTTCCAGAATGGGAAAGATGTGAAAAACCCTTGACACGATTGCATGTCACTTACGAAG
GTACCATAGAAGAAAATGGCCAAGGCATGCTACAGGTGGATTTTGCAAATCGTTTTGTTGGAGGTGGTGT
AACCAGTGCAGGACTTGTGCAAGAAGAAATCCGCTTTTTAATCAATCCTGAGTTGATTATTTCACGGCTC
TTCACTGAGGTGCTGGATCACAATGAATGTCTAATTATCACAGGTACTGAGCAGTACAGTGAATACACAG
GCTATGCTGAGACATATCGTTGGTCCCGGAGCCACGAAGATGGGAGTGAAAGGGACGACTGGCAGCGGCG
CTGCACTGAGATCGTTGCCATCGATGCTCTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAA
ATGAGACGCGAGCTGAACAAGGCTTACTGTGGATTTCTCCGTCCTGGAGTTTCTTCAGAGAATCTTTCTG
CAGTGGCCACAGGAAACTGGGGCTGTGGTGCCTTTGGGGGTGATGCCAGGTTAAAAGCCTTAATACAGAT
ATTGGCAGCTGCTGCAGCTGAGCGAGATGTGGTTTATTTCACCTTTGGGGACTCAGAATTGATGAGAGAC
ATTTACAGCATGCACATTTTCCTTACTGAAAGGAAACTCACTGTTGGAGATGTGTATAAGCTGTTGCTAC
GATACTACAATGAAGAATGCAGAAACTGTTCCACCCCTGGACCAGACATCAAGCTTTATCCATTCATATA
CCATGCTGTCGAGTCCTGTGCAGAGACCGCTGACCATTCAGGGCAAAGGACAGGGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208530 protein sequence
Red=Cloning site Green=Tags(s)

MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSAT
SLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTE
KSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQF
LTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNK
LTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDAD
IEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKA
EDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLR
AGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKY
NVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQI
ASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQ
SLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRL
FTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEK
MRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRD
IYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003631
ORF Size 2928 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003631.5
RefSeq Size 4276 bp
RefSeq ORF 2931 bp
Locus ID 8505
UniProt ID Q86W56
Cytogenetics 10q11.23
Domains PARG
MW 111.1 kDa
Gene Summary Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

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