PARG (NM_003631) Human Untagged Clone

CAT#: SC117822

PARG (untagged)-Human poly (ADP-ribose) glycohydrolase (PARG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARG
• Synonyms: PARG99
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117822 sequence for NM_003631 edited (data generated by NextGen Sequencing)
  ATGAATGCGGGCCCCGGCTGTGAACCCTGCACCAAGCGACCCCGCTGGGGCGCCGCTACA
  ACTTCGCCGGCTGCTTCGGACGCCCGGAGCTTTCCCAGCAGGCAGAGGCGCGTCCTCGAC
  CCCAAGGACGCTCACGTGCAGTTCAGGGTCCCACCGTCCTCGCCAGCCTGCGTCCCAGGG
  CGGGCGGGACAGCACAGAGGCAGCGCCACCTCGCTTGTTTTCAAACAAAAGACTATTACC
  AGTTGGATGGACACTAAAGGAATCAAGACAGCGGAATCAGAAAGTTTGGATAGTAAAGAA
  AACAACAATACAAGAATAGAATCCATGATGAGTTCTGTACAAAAAGATAACTTTTACCAA
  CATAATGTAGAAAAATTAGAAAATGTTTCTCAGCTAAGTCTTGATAAGTCACCCACTGAA
  AAAAGTACACAGTATTTGAACCAGCATCAGACTGCAGCAATGTGTAAGTGGCAAAATGAA
  GGGAAACACACGGAGCAGCTTTTGGAAAGTGAACCTCAAACAGTAACCCTGGTACCAGAG
  CAGTTTAGTAATGCTAACATTGATCGGTCACCTCAAAATGATGATCACAGTGACACAGAT
  AGTGAAGAGAATAGAGACAATCAACAGTTTCTCACAACTGTAAAGCTTGCAAATGCAAAG
  CAGACTACAGAAGATGAACAGGCCAGAGAAGCCAAAAGCCACCAGAAGTGCAGCAAGTCT
  TGCGATCCTGGGGAAGACTGTGCAAGTTGTCAGCAAGATGAGATAGATGTGGTGCCAGAG
  AGTCCATTGTCAGATGTTGGCTCTGAGGATGTTGGTACTGGGCCAAAAAATGACAACAAA
  TTGACTAGACAAGAAAGTTGCCTAGGAAATTCTCCTCCATTTGAGAAGGAAAGTGAACCC
  GAGTCACCGATGGATGTGGATAATTCTAAAAATAGTTGTCAAGACTCAGAAGCAGATGAG
  GAGACAAGTCCAGGTTTTGATGAACAAGAAGATGGTAGTTCCTCCCAAACAGCAAATAAA
  CCTTCAAGGTTCCAAGCAAGAGACGCTGACATTGAATTTAGGAAACGGTACTCTACTAAG
  GGCGGTGAAGTTAGATTACATTTCCAATTTGAAGGAGGAGAGAGTCGCACTGGAATGAAT
  GATTTAAATGCTAAACTACCTGGAAATATTTCTAGCCTGAATGTAGAATGCAGAAATTCT
  AAGCAACATGGAAAAAAGGATTCTAAAATCACAGATCATTTCATGAGACTGCCCAAAGCA
  GAGGACAGAAGAAAAGAACAGTGGGAAACCAAACATCAAAGAACAGAAAGGAAGATCCCT
  AAATACGTTCCACCTCACCTTTCTCCAGATAAGAAGTGGCTTGGAACTCCCATTGAGGAG
  ATGAGAAGAATGCCTCGGTGTGGGATCCGGCTGCCTCTCTTGAGACCATCTGCCAATCAC
  ACAGTAACTATTCGGGTAGATCTTTTGCGAGCAGGAGAAGTTCCTAAACCTTTTCCAACA
  CATTATAAAGATTTGTGGGATAACAAGCATGTTAAAATGCCTTGTTCAGAACAAAATTTG
  TACCCAGTGGAAGATGAGAATGGTGAGCGAACTGCGGGGAGCCGGTGGGAGCTCATTCAG
  ACTGCACTTCTCAACAAATTTACACGACCCCAAAACTTGAAGGATGCTATTCTGAAATAC
  AATGTGGCATATTCTAAGAAATGGGACTTTACAGCTTTGATCGATTTCTGGGATAAGGTA
  CTTGAAGAAGCAGAAGCTCAACATTTATATCAGTCCATCTTGCCTGATATGGTGAAAATT
  GCACTCTGTCTGCCAAATATTTGCACCCAGCCAATACCACTCCTGAAACAGAAGATGAAT
  CATTCCATCACAATGTCGCAGGAACAGATTGCCAGTCTTTTAGCTAATGCTTTCTTCTGC
  ACATTTCCACGACGAAATGCTAAGATGAAATCGGAGTATTCTAGTTACCCAGACATTAAC
  TTCAATCGATTGTTTGAGGGACGTTCATCAAGGAAACCGGAGAAACTTAAAACGCTCTTC
  TGCTACTTTAGAAGAGTCACAGAGAAAAAACCTACTGGGTTGGTGACATTTACAAGACAG
  AGTCTTGAAGATTTTCCAGAATGGGAAAGATGTGAAAAACCCTTGACACGATTGCATGTC
  ACTTACGAAGGTACCATAGAAGAAAATGGCCAAGGCATGCTACAGGTGGATTTTGCAAAT
  CGTTTTGTTGGAGGTGGTGTAACCAGTGCAGGACTTGTGCAAGAAGAAATCCGCTTTTTA
  ATCAATCCTGAGTTGATTATTTCACGGCTCTTCACTGAGGTGCTGGATCACAATGAATGT
  CTAATTATCACAGGTACTGAGCAGTACAGTGAATACACAGGCTATGCTGAGACATATCGT
  TGGTCCCGGAGCCACGAAGATGGGAGTGAAAGGGACGACTGGCAGCGGCGCTGCACTGAG
  ATCGTTGCCATCGATGCTCTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAA
  ATGAGACGCGAGCTGAACAAGGCTTACTGTGGATTTCTCCGTCCTGGAGTTTCTTCAGAG
  AATCTTTCTGCAGTGGCCACAGGAAACTGGGGCTGTGGTGCCTTTGGGGGTGATGCCAGG
  TTAAAAGCCTTAATACAGATATTGGCAGCTGCTGCAGCTGAGCGAGATGTGGTTTATTTC
  ACCTTTGGGGACTCAGAATTGATGAGAGACATTTACAGCATGCACATTTTCCTTACTGAA
  AGGAAACTCACTGTTGGAGATGTGTATAAGCTGTTGCTACGATACTACAATGAAGAATGC
  AGAAACTGTTCCACCCCTGGACCAGACATCAAGCTTTATCCATTCATATACCATGCTGTC
  GAGTCCTGTGCAGAGACCGCTGACCATTCAGGGCAAAGGACAGGGACCTGA
  
  Clone variation with respect to NM_003631.2
  669 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003631 unedited
  TGTATACGACTCACTTATAGGGCGGCCGCGATTTGGCACGAGGGGCAGGTGGGGTGCCAG
  TGGAAGAGGGAAGGCAGGCGAGTGTTTACGGCCTGACTTGGGAGGCCGGCGGATCAGCAA
  TTGCAGAAGCAGGCAGCGGCAGAGAGGGAATGGTGCAGGCAGGCGCTGAGAAGGAGGCGC
  AGTCCATCTCTCTCAGGGTTAGTGAATGAGGCTCTCCGCCCGGGCCGGCCCGGGGACAGT
  GCGCTGCTGGTCCCAGCATGAATGCGGGCCCCGGCTGTGAACCCTGCACCAAGCGACCCC
  GCTGGGGCGCCGCTACAACTTCGCCGGCTGCTTCGGACGCCCGGAGCTTTCCCAGCAGGC
  AGAGGCGCGTCCTCGACCCCAAGGACGCTCACGTGCAGTTCAGGGTCCCACCGTCCTCGC
  CAGCCTGCGTCCCAGGGCGGGCGGGACAGCACAGAGGCAGCGCCACCTCGCTTGTTTTCA
  AACAAAAGACTATTACCAGTTGGATGGACACTAAAGGAATCAAGACAGCGGAATCAGAAA
  GTTTGGATAGTAAAGAAAACAACAATACAAGAATAGAATCCATGATGAGTTCTGTACAAA
  AAGATAACTTTTACCAACATAATGTAGAAAAATTAGAAAATGTTTCTCAGCTAAGTCTTG
  ATAAGTCACCCACTGGAAAAAGTACACAGTATTTGAACCAGCATCAGACTGCAGNCATGG
  TGTAAGTGGCANAATGAAGGGAAACACACGGAGCAGCTTTGGNAAAGTGAACCTCAACAG
  TAACCCTGGTACCAGAGCAGNTTAGTAATGCTACCATTGATCGGNCACCTCAAATGATGA
  TCCCAGTGCACAGATAGTGAAGAGATAGAGACATCACAGTTTCTACACTGTAAAGCTTGC
  AATGCAAGCAACTACAGAGATGACAGCCANAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003631 unedited
  NNCCTACTTGNAAGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTCCCACCAGTCCAATTTTATTTAAATTTTATGGGCTCACATATAGACA
  CACTTAAAGAGCATACGTTTATTTATACATATTTAAAATATAGATGATTTGGGAGTTTCT
  GACAAAGGGATTTATTAAGGAAAATTCATTCACTATATAACCTTTGAATACCAAAAAAAC
  TTCAATATGTACAAATCAGCATGAAGTTCCCAGTTCTCAAACTTTGGTTATATGAATATG
  CCTCAAGCCCCAAAAAAAAAACATTAGCCCTAATTTAAAAAGAAAAAAGTACCAAGAAAC
  AAAAAAAATCTCAAATTTTCTTGAATCGGTTTTTTTTACACCACATCAGGAAAACCTCCT
  TTTATTCCCACATTTCCTTTCATCTAAGGCAGTCTCCAAAACTGGTATAAAAAAATCCTG
  TCCAGAGGAAAAAAGACAGACAAACCCTTACAGACAAAAATGATCCCTCAAAGCACAATT
  TCTGGAAATTATTCTATCTTAAATAAAGAAACTATTTTAACCTAGGAGGTTCGCTTTATG
  CCCCACATATGACAAAACCCCCAATTATTAATCCCCAGAATTCAAAAACTTTAACATGGC
  CGCCATTTTAACAGTCGAGTGGAAGGCTCCAGGGAAATTTTTATCACAATCCGTTAAAGA
  GATCCCTGGATTTCAACCTGCTTCCTGGCACCCCTGGTATCTGCGGCGTTGCCAAAGCCC
  CCGTTATAAATTATGGGCCAGACAAAAAAAACCCGACGGGCTTGAACACACCTTTCATGG
  GCATCTCACAAATTATCGCGGGACCCAATGAATCACCTCTCAGAAAGGGAAACCTGCATC
  AAAACCAAATTCAAN
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003631
• Insert Size: 4420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003631.2, NP_003622.2
• RefSeq Size: 4276 bp
• RefSeq ORF: 2931 bp
• Locus ID: 8505
• UniProt ID: Q86W56
• Cytogenetics: 10q11.23
• Domains: PARG
• Gene Summary: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (a).