PARG Mouse Monoclonal Antibody [Clone ID: OTI6F4]

CAT#: TA808614

PARG mouse monoclonal antibody, clone OTI6F4 (formerly 6F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PARG (Cat# RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG (Cat# TA808614)(1:2000). Positive lysates LY418533 (100ug) and LC418533 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-PARG monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PARG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808614) (1:150)

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Specifications

Product Data

• Clone Name: OTI6F4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-367 of human PARG(NP_003622) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 110.9 kDa
• Gene Name: poly(ADP-ribose) glycohydrolase
• Database Link:
  NP_003622
  Entrez Gene 26430 MouseEntrez Gene 8505 Human
  UniProt ID Q86W56
• Background: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq, Jul 2008]
• Synonyms: PARG99