PARG (NM_003631) Human Untagged Clone

CAT#: SC323845

PARG (untagged)-Human poly (ADP-ribose) glycohydrolase (PARG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARG
• Synonyms: PARG99
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003631.2 AAGCGGCGCATTGAGGCAGGTGGGGTGCCAGTGGAAGAGGGAAGGCAGGCGAGTGTTTAC
  GGCCTGACTTGGGAGGCCGGCGGATCAGCAATTGCAGAAGCAGGCAGCGGCAGAGAGGGA
  ATGGTGCAGGCAGGCGCTGAGAAGGAGGCGCAGTCCATCTCTCTCAGGGTTAGTGAATGA
  GGCTCTCCGCCCGGGCCGGCCCGGGGACAGTGCGCTGCTGGTCCCAGCATGAATGCGGGC
  CCCGGCTGTGAACCCTGCACCAAGCGACCCCGCTGGGGCGCCGCTACAACTTCGCCGGCT
  GCTTCGGACGCCCGGAGCTTTCCCAGCAGGCAGAGGCGCGTCCTCGACCCCAAGGACGCT
  CACGTGCAGTTCAGGGTCCCACCGTCCTCGCCAGCCTGCGTCCCAGGGCGGGCGGGACAG
  CACAGAGGCAGCGCCACCTCGCTTGTTTTCAAACAAAAGACTATTACCAGTTGGATGGAC
  ACTAAAGGAATCAAGACAGCGGAATCAGAAAGTTTGGATAGTAAAGAAAACAACAATACA
  AGAATAGAATCCATGATGAGTTCTGTACAAAAAGATAACTTTTACCAACATAATGTAGAA
  AAATTAGAAAATGTTTCTCAGCTAAGTCTTGATAAGTCACCCACTGAAAAAAGTACACAG
  TATTTGAACCAGCATCAGACTGCAGCAATGTGTAAGTGGCAAAATGAAGGGAAACACACG
  GAGCAGCTTTTGGAAAGTGAACCTCAAACAGTAACCCTGGTACCAGAGCAGTTTAGTAAT
  GCTAACATTGATCGGTCACCTCAAAATGATGATCACAGTGACACAGATAGTGAAGAGAAT
  AGAGACAATCAACAGTTTCTCACAACTGTAAAGCTTGCAAATGCAAAGCAGACTACAGAA
  GATGAACAGGCCAGAGAAGCCAAAAGCCACCAGAAGTGCAGCAAGTCTTGCGATCCTGGG
  GAAGACTGTGCAAGTTGTCAGCAAGATGAGATAGATGTGGTGCCAGAGAGTCCATTGTCA
  GATGTTGGCTCTGAGGATGTTGGTACTGGGCCAAAAAATGACAACAAATTGACTAGACAA
  GAAAGTTGCCTAGGAAATTCTCCTCCATTTGAGAAGGAAAGTGAACCCGAGTCACCGATG
  GATGTGGATAATTCTAAAAATAGTTGTCAAGACTCAGAAGCAGATGAGGAGACAAGTCCA
  GGTTTTGATGAACAAGAAGATGGTAGTTCCTCCCAAACAGCAAATAAACCTTCAAGGTTC
  CAAGCAAGAGACGCTGACATTGAATTTAGGAAACGGTACTCTACTAAGGGCGGTGAAGTT
  AGATTACATTTCCAATTTGAAGGAGGAGAGAGTCGCACTGGAATGAATGATTTAAATGCT
  AAACTACCTGGAAATATTTCTAGCCTGAATGTAGAATGCAGAAATTCTAAGCAACATGGA
  AAAAAGGATTCTAAAATCACAGATCATTTCATGAGACTGCCCAAAGCAGAGGACAGAAGA
  AAAGAACAGTGGGAAACCAAACATCAAAGAACAGAAAGGAAGATCCCTAAATACGTTCCA
  CCTCACCTTTCTCCAGATAAGAAGTGGCTTGGAACTCCCATTGAGGAGATGAGAAGAATG
  CCTCGGTGTGGGATCCGGCTGCCTCTCTTGAGACCATCTGCCAATCACACAGTAACTATT
  CGGGTAGATCTTTTGCGAGCAGGAGAAGTTCCTAAACCTTTTCCAACACATTATAAAGAT
  TTGTGGGATAACAAGCATGTTAAAATGCCTTGTTCAGAACAAAATTTGTACCCAGTGGAA
  GATGAGAATGGTGAGCGAACTGCGGGGAGCCGGTGGGAGCTCATTCAGACTGCACTTCTC
  AACAAATTTACACGACCCCAAAACTTGAAGGATGCTATTCTGAAATACAATGTGGCATAT
  TCTAAGAAATGGGACTTTACAGCTTTGATCGATTTCTGGGATAAGGTACTTGAAGAAGCA
  GAAGCTCAACATTTATATCAGTCCATCTTGCCTGATATGGTGAAAATTGCACTCTGTCTG
  CCAAATATTTGCACCCAGCCAATACCACTCCTGAAACAGAAGATGAATCATTCCATCACA
  ATGTCGCAGGAACAGATTGCCAGTCTTTTAGCTAATGCTTTCTTCTGCACATTTCCACGA
  CGAAATGCTAAGATGAAATCGGAGTATTCTAGTTACCCAGACATTAACTTCAATCGATTG
  TTTGAGGGACGTTCATCAAGGAAACCGGAGAAACTTAAAACGCTCTTCTGCTACTTTAGA
  AGAGTCACAGAGAAAAAACCTACTGGGTTGGTGACATTTACAAGACAGAGTCTTGAAGAT
  TTTCCAGAATGGGAAAGATGTGAAAAACCCTTGACACGATTGCATGTCACTTACGAAGGT
  ACCATAGAAGAAAATGGCCAAGGCATGCTACAGGTGGATTTTGCAAATCGTTTTGTTGGA
  GGTGGTGTAACCAGTGCAGGACTTGTGCAAGAAGAAATCCGCTTTTTAATCAATCCTGAG
  TTGATTATTTCACGGCTCTTCACTGAGGTGCTGGATCACAATGAATGTCTAATTATCACA
  GGTACTGAGCAGTACAGTGAATACACAGGCTATGCTGAGACATATCGTTGGTCCCGGAGC
  CACGAAGATGGGAGTGAAAGGGACGACTGGCAGCGGCGCTGCACTGAGATCGTTGCCATC
  GATGCTCTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAAATGAGACGCGAG
  CTGAACAAGGCTTACTGTGGATTTCTCCGTCCTGGAGTTTCTTCAGAGAATCTTTCTGCA
  GTGGCCACAGGAAACTGGGGCTGTGGTGCCTTTGGGGGTGATGCCAGGTTAAAAGCCTTA
  ATACAGATATTGGCAGCTGCTGCAGCTGAGCGAGATGTGGTTTATTTCACCTTTGGGGAC
  TCAGAATTGATGAGAGACATTTACAGCATGCACATTTTCCTTACTGAAAGGAAACTCACT
  GTTGGAGATGTGTATAAGCTGTTGCTACGATACTACAATGAAGAATGCAGAAACTGTTCC
  ACCCCTGGACCAGACATCAAGCTTTATCCATTCATATACCATGCTGTCGAGTCCTGTGCA
  GAGACCGCTGACCATTCAGGGCAAAGGACAGGGACCTGAGGAGCCGAGCGAATAGCATCT
  CCTCCCACCTCCCACCAGAGACGTCCTGTTTGAGCTGTCAGGTGTAATATATGAATTGAC
  TTAAGTTAATATAAATGTGTACATAATCCACATTTGTAGTCAAGGACGCAATCTCTTCCA
  CACATGTGCAGTTGTCAGTTGGTACATCTAAACTCCCTCCATCCTGACTCACGTGGACTT
  AGATATGTTTTGTTTCTATTTTCTTCTATTTCAGTTTTTCATTCTTTGATGTTTATTTCT
  TTTGTCCATCAGATCTCTTGTGAAATCCCATGGAAGGTTGTGCTCAGCCTGTCGGGTCTC
  TTTCTTCCTGCCCATATATTATACCAGTTGCTTCTGCAGCCCGCAGATGCCAGCGATGCC
  AGGAAACAAGTTGAAATCCAGGAATCTCTTTAACTGATTTTGCTAAAAATCTCCCTGTGA
  GCCTTCCACTCAACTCTTAATATGCTTGCATTGTTTAAGTTTTTAAATTCTGAAAATTAA
  TAATTAGGGTTTTTTTCATATGTGTTGCATAATGCAAACCTCCTAGGTTAAAATAGTTTC
  TTTATTTAAGATAGAATAATTTCCAGAAATTGTACTTTTGAGGTATCATTTTTATCTGTA
  ATGGTTTGTCTGTCTTTTTTCCTCTGATCAGTATTTTTTTATACCAGTTTTGGAGACTGG
  CTGAGATGAAAGGAAATGTGGAATAAAAGGAGGTTTTCCTGATGTGGTGTAAAGAAAACA
  GATTCAAGAGAATTGAAGATTTTTTTTGTTTCTTGGTACTTTTTTCTTTTTAAATTAGGA
  CTAATGTTTCTTTTGTGGTGCTTGAGGCATATTCATATAACCAAAGTTTGAGAACTGGGA
  ACTTCATGCTGATTTGTACATATTGAAGTTTCTCTGGTATTCAAAGGTTATATAGTGAAT
  GAATTTTCATTAATAAATCACTTTGTCAGAAACTCCCATATCATCTATATTTTATATATG
  TATATATAAACGTATGCTCTTTAAGTGTGTCTATATGTGAGCACATAAAATCTAAATAAA
  ATTGGACTGGTGGGAAACAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003631
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003631.2, NP_003622.2
• RefSeq Size: 4276 bp
• RefSeq ORF: 2931 bp
• Locus ID: 8505
• UniProt ID: Q86W56
• Cytogenetics: 10q11.23
• Domains: PARG
• Gene Summary: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (a).