IL13 receptor alpha 2 (IL13RA2) (NM_000640) Human Tagged ORF Clone

CAT#: RC207672

IL13RA2 (Myc-DDK-tagged)-Human interleukin 13 receptor, alpha 2 (IL13RA2)

Product Images

Circular map for RC207672

• 

  Western blot validation of overexpression lysate (Cat# LY424589) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207672 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL13 receptor alpha 2
• Synonyms: CD213A2; CT19; IL-13R; IL13BP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207672 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCGTTTGCTTGGCTATCGGATGCTTATATACCTTTCTGATAAGCACAACATTTGGCTGTACTT
  CATCTTCAGACACCGAGATAAAAGTTAACCCTCCTCAGGATTTTGAGATAGTGGATCCCGGATACTTAGG
  TTATCTCTATTTGCAATGGCAACCCCCACTGTCTCTGGATCATTTTAAGGAATGCACAGTGGAATATGAA
  CTAAAATACCGAAACATTGGTAGTGAAACATGGAAGACCATCATTACTAAGAATCTACATTACAAAGATG
  GGTTTGATCTTAACAAGGGCATTGAAGCGAAGATACACACGCTTTTACCATGGCAATGCACAAATGGATC
  AGAAGTTCAAAGTTCCTGGGCAGAAACTACTTATTGGATATCACCACAAGGAATTCCAGAAACTAAAGTT
  CAGGATATGGATTGCGTATATTACAATTGGCAATATTTACTCTGTTCTTGGAAACCTGGCATAGGTGTAC
  TTCTTGATACCAATTACAACTTGTTTTACTGGTATGAGGGCTTGGATCATGCATTACAGTGTGTTGATTA
  CATCAAGGCTGATGGACAAAATATAGGATGCAGATTTCCCTATTTGGAGGCATCAGACTATAAAGATTTC
  TATATTTGTGTTAATGGATCATCAGAGAACAAGCCTATCAGATCCAGTTATTTCACTTTTCAGCTTCAAA
  ATATAGTTAAACCTTTGCCGCCAGTCTATCTTACTTTTACTCGGGAGAGTTCATGTGAAATTAAGCTGAA
  ATGGAGCATACCTTTGGGACCTATTCCAGCAAGGTGTTTTGATTATGAAATTGAGATCAGAGAAGATGAT
  ACTACCTTGGTGACTGCTACAGTTGAAAATGAAACATACACCTTGAAAACAACAAATGAAACCCGACAAT
  TATGCTTTGTAGTAAGAAGCAAAGTGAATATTTATTGCTCAGATGACGGAATTTGGAGTGAGTGGAGTGA
  TAAACAATGCTGGGAAGGTGAAGACCTATCGAAGAAAACTTTGCTACGTTTCTGGCTACCATTTGGTTTC
  ATCTTAATATTAGTTATATTTGTAACCGGTCTGCTTTTGCGTAAGCCAAACACCTACCCAAAAATGATTC
  CAGAATTTTTCTGTGATACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207672 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFVCLAIGCLYTFLISTTFGCTSSSDTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYE
  LKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKV
  QDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDF
  YICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDD
  TTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLLRFWLPFGF
  ILILVIFVTGLLLRKPNTYPKMIPEFFCDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000640
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000640.3
• RefSeq Size: 1376 bp
• RefSeq ORF: 1143 bp
• Locus ID: 3598
• UniProt ID: Q14627
• Cytogenetics: Xq23
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Jak-STAT signaling pathway
• MW: 44.2 kDa
• Gene Summary: The protein encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13. [provided by RefSeq, Jul 2008]