GABA A Receptor beta 1 (GABRB1) (NM_000812) Human Tagged ORF Clone

CAT#: RC205166

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GABRB1 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 1 (GABRB1)



  "NM_000812" in other vectors (6)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GABRB1
Synonyms DEE45; EIEE45
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205166 representing NM_000812
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGACAGTACAAAATCGAGAGAGTCTGGGGCTTCTCTCTTTCCCTGTGATGATTACCATGGTCTGTT
GTGCACACAGCACCAATGAACCCAGCAACATGTCATACGTGAAAGAGACAGTGGACAGATTGCTCAAAGG
ATATGACATTCGCTTGCGGCCGGACTTCGGAGGGCCCCCCGTCGACGTTGGGATGCGGATCGATGTCGCC
AGCATAGACATGGTCTCCGAAGTGAATATGGATTATACACTCACCATGTATTTCCAGCAGTCTTGGAAAG
ACAAAAGGCTTTCTTATTCTGGAATCCCACTGAACCTCACCCTAGACAATAGGGTAGCTGACCAACTCTG
TGTACCAGACACCTACTTTCTGAATGACAAGAAATCATTTGTGCATGGGGTCACAGTGAAAAATCGAATG
ATTCGACTGCATCCTGATGGAACAGTTCTCTATGGACTCCGAATCACAACCACAGCTGCATGTATGATGG
ATCTTCGAAGATATCCACTGGATGAGCAGAACTGCACCCTGGAGATCGAAAGTTATGGCTATACCACTGA
TGACATTGAATTTTACTGGAATGGAGGAGAAGGGGCAGTCACTGGTGTTAATAAAATCGAACTTCCTCAA
TTTTCAATTGTTGACTACAAGATGGTGTCTAAGAAGGTGGAGTTCACAACAGGAGCGTATCCACGACTGT
CACTAAGTTTTCGTCTAAAGAGAAACATTGGTTACTTCATTTTGCAAACCTACATGCCTTCTACACTGAT
TACAATTCTGTCCTGGGTGTCTTTTTGGATCAACTATGATGCATCTGCAGCCAGAGTCGCACTAGGAATC
ACGACGGTGCTTACAATGACAACCATCAGCACCCACCTCAGGGAGACCCTGCCAAAGATCCCTTATGTCA
AAGCGATTGATATTTATCTGATGGGTTGCTTTGTGTTTGTGTTCCTGGCTCTGCTGGAGTATGCCTTTGT
AAATTACATCTTCTTTGGGAAAGGCCCTCAGAAAAAGGGAGCTAGCAAACAAGACCAGAGTGCCAATGAG
AAGAATAAACTGGAGATGAATAAAGTCCAGGTCGACGCCCACGGTAACATTCTCCTCAGCACCCTGGAAA
TCCGGAATGAGACGAGTGGCTCGGAAGTGCTCACGAGCGTGAGCGACCCCAAGGCCACCATGTACTCCTA
TGACAGCGCCAGCATCCAGTACCGCAAGCCCCTGAGCAGCCGCGAGGCCTACGGGCGCGCCCTGGACCGG
CACGGGGTACCCAGCAAGGGGCGCAACCGCAGGCGTGCCTCCCAGCTCAAAGTCAAGATCCCCGACTTGA
CTGATGTGAATTCCATAGACAAGTGGTCCCGAATGTTTTTCCCCATCACCTTTTCTCTTTTTAATGTCGT
CTATTGGCTTTACTATGTACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205166 representing NM_000812
Red=Cloning site Green=Tags(s)

MWTVQNRESLGLLSFPVMITMVCCAHSTNEPSNMSYVKETVDRLLKGYDIRLRPDFGGPPVDVGMRIDVA
SIDMVSEVNMDYTLTMYFQQSWKDKRLSYSGIPLNLTLDNRVADQLCVPDTYFLNDKKSFVHGVTVKNRM
IRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWNGGEGAVTGVNKIELPQ
FSIVDYKMVSKKVEFTTGAYPRLSLSFRLKRNIGYFILQTYMPSTLITILSWVSFWINYDASAARVALGI
TTVLTMTTISTHLRETLPKIPYVKAIDIYLMGCFVFVFLALLEYAFVNYIFFGKGPQKKGASKQDQSANE
KNKLEMNKVQVDAHGNILLSTLEIRNETSGSEVLTSVSDPKATMYSYDSASIQYRKPLSSREAYGRALDR
HGVPSKGRNRRRASQLKVKIPDLTDVNSIDKWSRMFFPITFSLFNVVYWLYYVH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000812
ORF Size 1422 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000812.4
RefSeq Size 1925 bp
RefSeq ORF 1425 bp
Locus ID 2560
UniProt ID P18505
Cytogenetics 4p12
Domains Neur_chan_memb, Neur_chan_LBD
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 54.1 kDa
Gene Summary The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 1 subunit. It is mapped to chromosome 4p12 in a cluster comprised of genes encoding alpha 4, alpha 2 and gamma 1 subunits of the GABA A receptor. Alteration of this gene is implicated in the pathogenetics of schizophrenia. [provided by RefSeq, Jul 2008]

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