TST (NM_003312) Human Tagged ORF Clone

CAT#: RC204002

TST (Myc-DDK-tagged)-Human thiosulfate sulfurtransferase (rhodanese) (TST), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY418774) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204002 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TST protein (Cat# TP304002). The protein was produced from HEK293T cells transfected with TST cDNA clone (Cat# RC204002) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TST
• Synonyms: RDS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204002 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCATCAGGTGCTCTACCGGGCGCTGGTCTCCACCAAGTGGCTGGCGGAGTCCATCAGGACTGGCA
  AGCTGGGGCCCGGCCTGCGGGTGCTGGACGCGTCCTGGTACTCACCAGGCACCCGAGAGGCCCGCAAGGA
  GTACCTCGAGCGCCACGTACCCGGCGCCTCTTTCTTTGACATAGAAGAGTGCCGGGACACGGCGTCGCCC
  TACGAGATGATGCTGCCCAGCGAGGCTGGCTTCGCCGAGTATGTGGGCCGCCTGGGCATCAGCAACCACA
  CGCACGTGGTGGTGTATGATGGTGAACACCTGGGCAGCTTCTATGCTCCCCGGGTCTGGTGGATGTTCCG
  TGTGTTTGGCCACCGCACCGTATCAGTGCTCAATGGTGGCTTCCGGAACTGGCTGAAGGAGGGCCACCCG
  GTGACATCCGAGCCCTCACGCCCAGAACCGGCCGTCTTCAAAGCCACACTGGACCGCTCCCTGCTCAAGA
  CCTACGAGCAGGTGCTGGAGAACCTTGAATCTAAGAGGTTCCAGCTGGTGGATTCAAGGTCTCAAGGGCG
  GTTCCTGGGCACCGAGCCGGAGCCGGATGCAGTAGGACTGGACTCGGGCCATATCCGTGGTGCCGTCAAC
  ATGCCTTTCATGGACTTCCTGACTGAGGATGGCTTCGAGAAGGGCCCAGAAGAGCTCCGTGCTCTGTTCC
  AGACCAAGAAGGTGGATCTCTCGCAGCCTCTCATTGCCACGTGCCGCAAGGGAGTCACCGCCTGCCACGT
  GGCCTTGGCTGCCTACCTCTGCGGCAAGCCTGATGTGGCCGTGTACGATGGCTCCTGGTCCGAGTGGTTT
  CGCCGGGCCCCCCCAGAGAGCCGTGTGTCCCAGGGAAAGTCTGAGAAGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204002 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDTASP
  YEMMLPSEAGFAEYVGRLGISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHP
  VTSEPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVN
  MPFMDFLTEDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWF
  RRAPPESRVSQGKSEKA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003312
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003312.6
• RefSeq Size: 1143 bp
• RefSeq ORF: 894 bp
• Locus ID: 7263
• UniProt ID: Q16762
• Cytogenetics: 22q12.3
• Domains: RHOD
• Protein Families: Druggable Genome
• MW: 33.4 kDa
• Gene Summary: This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]