CYPIVF11 (CYP4F11) (NM_021187) Human Tagged ORF Clone

CAT#: RC203705

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CYP4F11 (Myc-DDK-tagged)-Human cytochrome P450, family 4, subfamily F, polypeptide 11 (CYP4F11), transcript variant 1



Reconstitution Protocol

USD 488.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CYP4F11
Synonyms CYPIVF11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203705 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTGCTTCTGCTGC
TGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACCTTCTATGACAACTGCCGCCG
CCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTTTGGGGACACCAGGGCCTGGTCACTCCCACG
GAAGAGGGCATGAAGACATTGACCCAGCTGGTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTC
CTACCTTCCCCCTCCTCATTTTATGCCACCCTGACATCATCCGGCCTATCACCAGTGCCTCAGCTGCTGT
CGCACCCAAGGATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTGAAGCCTTATA
TGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGCCTGGCCTCAGAGGGCAGCGC
CAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTGGACAGTCTGCAGAAATGTGTCTTCAGCTTT
GAAAGCAATTGTCAGGAGAAGCCCAGTGAATATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAA
AGAGAAACCAGCAGATTCTCTTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCG
CAGGGCCTGCCACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCCACT
CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGATGTGCTTCTGC
TGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCAGAAGCTGACACCTTCATGTT
TGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTCCTATACCACCTTGCAAAGCACCCAGAATAC
CAGGAACAGTGCCGGCAAGAAGTGCAAGAGCTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACG
ACCTGGCCCAGCTGCCCTTCCTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGT
CATCTCCCGATGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTACGACCCCTTCC
GTTTCAACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCCTTCTCGGCAGGGCCCAGAAA
CTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTGGTCCTGGCGCTCACGCTGCTGCACTTCCGC
ATCCTGCCGACCCACACTGAACCCCGCAGGAAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGC
TGCGGGTGGAGCCCCTGGGTGCGAACTCACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203705 protein sequence
Red=Cloning site Green=Tags(s)

MPQLSLSWLGLGPVAASPWLLLLLVGGSWLLARVLAWTYTFYDNCRRLQCFPQPPKQNWFWGHQGLVTPT
EEGMKTLTQLVTTYPQGFKLWLGPTFPLLILCHPDIIRPITSASAAVAPKDMIFYGFLKPWLGDGLLLSG
GDKWSRHRRMLTPAFHFNILKPYMKIFNKSVNIMHDKWQRLASEGSARLDMFEHISLMTLDSLQKCVFSF
ESNCQEKPSEYIAAILELSAFVEKRNQQILLHTDFLYYLTPDGQRFRRACHLVHDFTDAVIQERRRTLPT
QGIDDFLKNKAKSKTLDFIDVLLLSKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEY
QEQCRQEVQELLKDREPIEIEWDDLAQLPFLTMCIKESLRLHPPVPVISRCCTQDFVLPDGRVIPKGIVC
LINIIGIHYNPTVWPDPEVYDPFRFNQENIKERSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALTLLHFR
ILPTHTEPRRKPELILRAEGGLWLRVEPLGANSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021187
ORF Size 1572 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021187.3
RefSeq Size 3395 bp
RefSeq ORF 1575 bp
Locus ID 57834
UniProt ID Q9HBI6
Cytogenetics 19p13.12
Protein Families Druggable Genome, P450, Transmembrane
MW 60.2 kDa
Gene Summary This gene, CYP4F11, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F2, is approximately 16 kb away. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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