Phospholipase A2 IIA (PLA2G2A) (NM_000300) Human Tagged ORF Clone
PLA2G2A (Myc-DDK-tagged)-Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1
|Type||Human Tagged ORF Clone|
|Synonyms||MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC203250 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC203250 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||432 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1017 bp|
|RefSeq ORF||435 bp|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway|
|Gene Summary||The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]|
|cDNA Clone Resources|
|RC203250L1||Lenti ORF clone of Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1, Myc-DDK-tagged||
|RC203250L2||Lenti ORF clone of Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1, mGFP tagged||
|RC203250L3||Lenti ORF clone of Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1, Myc-DDK-tagged||
|RC203250L4||Lenti ORF clone of Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1, mGFP tagged||
|RG203250||PLA2G2A (GFP-tagged) - Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1||
|SC119954||PLA2G2A (untagged)-Human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A), transcript variant 1||