EZH1 (NM_001991) Human Tagged ORF Clone

CAT#: RC202367

EZH1 (Myc-DDK-tagged)-Human enhancer of zeste homolog 1 (Drosophila) (EZH1)

Product Images

Circular map for RC202367

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  Western blot validation of overexpression lysate (Cat# LY419594) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202367 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EZH1 protein (Cat# TP302367). The protein was produced from HEK293T cells transfected with EZH1 cDNA clone (Cat# RC202367) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EZH1
• Synonyms: KMT6B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202367 representing NM_001991
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATACCAAATCCCCCTACCTCCAAATGTATCACTTACTGGAAAAGAAAAGTGAAATCTGAATACA
  TGCGACTTCGACAACTTAAACGGCTTCAGGCAAATATGGGTGCAAAGGCTTTGTATGTGGCAAATTTTGC
  AAAGGTTCAAGAAAAAACCCAGATCCTCAATGAAGAATGGAAGAAGCTTCGTGTCCAACCTGTTCAGTCA
  ATGAAGCCTGTGAGTGGACACCCTTTTCTCAAAAAGTGTACCATAGAGAGCATTTTCCCGGGATTTGCAA
  GCCAACATATGTTAATGAGGTCACTGAACACAGTTGCATTGGTTCCCATCATGTATTCCTGGTCCCCTCT
  CCAACAGAACTTTATGGTAGAAGATGAGACGGTTTTGTGCAATATTCCCTACATGGGAGATGAAGTGAAA
  GAAGAAGATGAGACTTTTATTGAGGAGCTGATCAATAACTATGATGGGAAAGTCCATGGTGAAGAAGAGA
  TGATCCCTGGATCCGTTCTGATTAGTGATGCTGTTTTTCTGGAGTTGGTCGATGCCCTGAATCAGTACTC
  AGATGAGGAGGAGGAAGGGCACAATGACACCTCAGATGGAAAGCAGGATGACAGCAAAGAAGATCTGCCA
  GTAACAAGAAAGAGAAAGCGACATGCTATTGAAGGCAACAAAAAGAGTTCCAAGAAACAGTTCCCAAATG
  ACATGATCTTCAGTGCAATTGCCTCAATGTTCCCTGAGAATGGTGTCCCAGATGACATGAAGGAGAGGTA
  TCGAGAACTAACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATCGATGGCCCC
  AATGCCAAGTCTGTGCAGCGGGAGCAATCTCTGCACTCCTTCCACACACTTTTTTGCCGGCGCTGCTTTA
  AATACGACTGCTTCCTTCACCCTTTTCATGCCACCCCTAATGTATATAAACGCAAGAATAAAGAAATCAA
  GATTGAACCAGAACCATGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTATGCCATGCTC
  CACAACCCCCGCTCCAAGTGCTCTGGTCGTCGCCGGAGAAGGCACCACATAGTCAGTGCTTCCTGCTCCA
  ATGCCTCAGCCTCTGCTGTGGCTGAGACTAAAGAAGGAGACAGTGACAGGGACACAGGCAATGACTGGGC
  CTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACTCCCACAAAACAGAAGGCTAGTCCAGCCCCACCT
  CAACTCTGCGTAGTGGAAGCACCCTCGGAGCCTGTGGAATGGACTGGGGCTGAAGAATCTCTTTTTCGAG
  TCTTCCATGGCACCTACTTCAACAACTTCTGTTCAATAGCCAGGCTTCTGGGGACCAAGACGTGCAAGCA
  GGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAAGCTGCCAACAGATGAGCTCATGAACCCCTCA
  CAGAAGAAGAAAAGAAAGCACAGATTGTGGGCTGCACACTGCAGGAAGATTCAGCTGAAGAAAGATAACT
  CTTCCACACAAGTGTACAACTACCAACCCTGCGACCACCCAGACCGCCCCTGTGACAGCACCTGCCCCTG
  CATCATGACTCAGAATTTCTGTGAGAAGTTCTGCCAGTGCAACCCAGACTGTCAGAATCGTTTCCCTGGC
  TGTCGCTGTAAGACCCAGTGCAATACCAAGCAATGTCCTTGCTATCTGGCAGTGCGAGAATGTGACCCTG
  ACCTGTGTCTCACCTGTGGGGCCTCAGAGCACTGGGACTGCAAGGTGGTTTCCTGTAAAAACTGCAGCAT
  CCAGCGTGGACTTAAGAAGCACCTGCTGCTGGCCCCCTCTGATGTGGCCGGATGGGGCACCTTCATAAAG
  GAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTGGTGAGCTCATCTCTCAGGATGAGGCTGATC
  GACGCGGAAAGGTCTATGACAAATACATGTCCAGCTTCCTCTTCAACCTCAATAATGATTTTGTAGTGGA
  TGCTACTCGGAAAGGAAACAAAATTCGATTTGCAAATCATTCAGTGAATCCCAACTGTTATGCCAAAGTG
  GTCATGGTGAATGGAGACCATCGGATTGGGATCTTTGCCAAGAGGGCAATTCAAGCTGGCGAAGAGCTCT
  TCTTTGATTACAGGTACAGCCAAGCTGATGCTCTCAAGTACGTGGGGATCGAGAGGGAGACCGACGTCCT
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202367 representing NM_001991
  Red=Cloning site Green=Tags(s)
  
  MEIPNPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQPVQS
  MKPVSGHPFLKKCTIESIFPGFASQHMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVK
  EEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEEGHNDTSDGKQDDSKEDLP
  VTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNIDGP
  NAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEYAML
  HNPRSKCSGRRRRRHHIVSASCSNASASAVAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASPAPP
  QLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPS
  QKKKRKHRLWAAHCRKIQLKKDNSSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCNPDCQNRFPG
  CRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIK
  ESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKV
  VMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001991
• ORF Size: 2241 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001991.5
• RefSeq Size: 4640 bp
• RefSeq ORF: 2244 bp
• Locus ID: 2145
• UniProt ID: Q92800
• Cytogenetics: 17q21.2
• Domains: myb_DNA-binding, SET
• Protein Families: Druggable Genome, Transcription Factors
• MW: 85.1 kDa
• Gene Summary: EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]