STAMBPL1 (NM_020799) Human Tagged ORF Clone

CAT#: RC201884

STAMBPL1 (Myc-DDK-tagged)-Human STAM binding protein-like 1 (STAMBPL1)

Product Images

Circular map for RC201884

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  Western blot validation of overexpression lysate (Cat# LY402811) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201884 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STAMBPL1 protein (Cat# TP301884). The protein was produced from HEK293T cells transfected with STAMBPL1 cDNA clone (Cat# RC201884) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAMBPL1
• Synonyms: ALMalpha; AMSH-FP; AMSH-LP; bA399O19.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201884 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGCCTTTTACTGTGAATTCTCTGAAAAAGTTAGCTGCTATGCCTGACCATACAGATGTTTCCC
  TAAGCCCAGAAGAGCGAGTCCGTGCCCTAAGCAAGCTTGGTTGTAATATCACCATCAGTGAAGACATCAC
  TCCACGACGTTACTTTAGGTCTGGAGTAGAGATGGAGAGGATGGCGTCTGTGTATTTGGAAGAAGGAAAT
  TTGGAAAATGCCTTTGTTCTTTATAATAAATTTATAACCTTATTTGTAGAAAAGCTTCCTAACCATCGAG
  ATTACCAGCAATGTGCAGTACCTGAAAAGCAGGATATTATGAAGAAACTGAAGGAGATTGCATTCCCAAG
  GACAGATGAATTGAAAAACGACCTTTTAAAGAAATATAACGTAGAATACCAAGAATATTTGCAAAGCAAA
  AACAAATATAAAGCTGAAATTCTCAAAAAATTGGAGCATCAGAGATTGATAGAGGCAGAAAGGAAGCGGA
  TTGCTCAGATGCGCCAGCAGCAGCTAGAATCGGAGCAGTTTCTGTTTTTCGAAGATCAACTCAAGAAGCA
  AGAGTTAGCCCGAGGTCAAATGCGAAGTCAGCAAACCTCAGGGCTGTCAGAGCAGATTGATGGGAGCGCT
  TTGTCCTGCTTTTCCACACACCAGAACAATTCCTTGCTGAATGTATTTGCAGATCAACCTAATAAAAGTG
  ATGCAACCAATTATGCTAGCCACTCTCCTCCTGTAAACAGGGCCTTAACACCAGCTGCTACTCTAAGTGC
  TGTTCAGAATTTAGTGGTTGAAGGACTGCGATGTGTAGTTTTGCCAGAAGATCTTTGCCACAAATTTCTG
  CAACTGGCAGAATCTAATACAGTGAGAGGAATAGAAACCTGTGGAATACTCTGTGGAAAACTGACACATA
  ATGAATTTACTATTACCCATGTAATTGTGCCAAAGCAGTCTGCGGGACCAGACTATTGTGACATGGAGAA
  TGTAGAGGAATTATTCAATGTTCAGGATCAACATGATCTCCTCACTCTAGGATGGATCCATACACATCCC
  ACTCAAACTGCATTTTTATCCAGCGTTGATCTTCACACTCACTGTTCCTATCAACTCATGTTGCCAGAGG
  CCATTGCCATTGTTTGCTCACCAAAGCATAAAGACACTGGCATCTTCAGGCTCACCAATGCTGGCATGCT
  TGAGGTTTCTGCTTGTAAAAAAAAGGGCTTTCATCCACACACCAAGGAGCCCAGGCTGTTCAGTATATGC
  AAACATGTGTTGGTAAAAGACATAAAAATAATTGTGTTGGATCTGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201884 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDQPFTVNSLKKLAAMPDHTDVSLSPEERVRALSKLGCNITISEDITPRRYFRSGVEMERMASVYLEEGN
  LENAFVLYNKFITLFVEKLPNHRDYQQCAVPEKQDIMKKLKEIAFPRTDELKNDLLKKYNVEYQEYLQSK
  NKYKAEILKKLEHQRLIEAERKRIAQMRQQQLESEQFLFFEDQLKKQELARGQMRSQQTSGLSEQIDGSA
  LSCFSTHQNNSLLNVFADQPNKSDATNYASHSPPVNRALTPAATLSAVQNLVVEGLRCVVLPEDLCHKFL
  QLAESNTVRGIETCGILCGKLTHNEFTITHVIVPKQSAGPDYCDMENVEELFNVQDQHDLLTLGWIHTHP
  TQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHKDTGIFRLTNAGMLEVSACKKKGFHPHTKEPRLFSIC
  KHVLVKDIKIIVLDLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020799
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020799.2, NP_065850.1
• RefSeq Size: 2094 bp
• RefSeq ORF: 1311 bp
• Locus ID: 57559
• UniProt ID: Q96FJ0
• Cytogenetics: 10q23.31
• Protein Families: Druggable Genome, Protease
• MW: 49.8 kDa
• Gene Summary: Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.[UniProtKB/Swiss-Prot Function]