Nucleobindin 1 (NUCB1) (NM_006184) Human Tagged ORF Clone

CAT#: RC201786

NUCB1 (Myc-DDK-tagged)-Human nucleobindin 1 (NUCB1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUCB1 (Cat# RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1 (Cat# TA503868). Positive lysates LY416822 (100ug) and LC416822 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416822) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201786 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NUCB1 protein (Cat# TP301786). The protein was produced from HEK293T cells transfected with NUCB1 cDNA clone (Cat# RC201786) using MegaTran 2.0 (Cat# TT210002).

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  Anti-NUCB1 mouse monoclonal antibody (TA503868) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1 (RC201786).

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  HEK293T cells transfected with either RC201786 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUCB1 antibody (TA503868), and then analyzed by flow cytometry.

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUCB1
• Synonyms: CALNUC; NUC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201786 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCCTCTGGGCCCCGAGGAACCCTCCTTCTGTTGCCGCTGCTGCTGCTGCTCCTGCTTCGCGCCG
  TGCTGGCTGTCCCCCTGGAGCGAGGGGCGCCCAACAAGGAGGAGACCCCTGCGACTGAGAGTCCCGACAC
  AGGCCTGTACTACCACCGGTACCTCCAGGAGGTCATCGATGTACTGGAGACGGATGGGCATTTCCGAGAG
  AAGCTGCAGGCTGCCAATGCGGAGGACATCAAGAGCGGGAAGCTGAGCCGAGAGCTGGACTTTGTCAGCC
  ACCACGTCCGCACCAAGCTGGATGAGCTCAAGCGACAGGAGGTGTCACGGCTGCGGATGCTGCTCAAGGC
  CAAGATGGACGCCGAGCAGGATCCCAATGTACAGGTGGATCATCTGAATCTCCTGAAACAGTTTGAACAC
  CTGGACCCTCAGAACCAGCATACATTCGAGGCCCGCGACCTGGAGCTGCTGATCCAGACGGCCACCCGGG
  ACCTTGCCCAGTACGACGCAGCCCATCATGAAGAGTTCAAGCGCTACGAGATGCTTAAGGAACACGAGAG
  ACGGCGTTATCTGGAGTCACTGGGAGAGGAGCAGAGAAAGGAGGCGGAGAGGAAGCTGGAAGAGCAACAG
  CGCCGGCACCGCGAGCACCCTAAAGTCAACGTGCCTGGCAGCCAAGCCCAGTTGAAGGAGGTGTGGGAGG
  AGCTGGATGGACTGGACCCCAACAGGTTTAACCCCAAGACCTTCTTCATACTGCATGATATCAACAGTGA
  TGGTGTCCTGGATGAGCAGGAGCTGGAGGCACTCTTCACCAAGGAGCTGGAGAAAGTGTACGACCCAAAG
  AATGAGGAGGACGACATGCGGGAGATGGAGGAGGAGCGACTGCGCATGCGGGAGCATGTGATGAAGAATG
  TGGACACCAACCAGGACCGCCTCGTGACCCTGGAGGAGTTCCTCGCATCCACTCAGAGGAAGGAGTTTGG
  GGACACCGGGGAGGGCTGGGAGACAGTGGAGATGCACCCTGCCTACACCGAGGAAGAGCTGAGGCGCTTT
  GAAGAGGAGCTGGCTGCCCGGGAGGCAGAGCTGAATGCCAAGGCCCAGCGCCTCAGCCAGGAGACAGAGG
  CTCTAGGGCGGTCCCAGGGCCGCCTGGAGGCCCAGAAGAGAGAGCTGCAGCAGGCTGTGCTGCACATGGA
  GCAGCGGAAGCAGCAGCAGCAGCAGCAGCAAGGCCACAAGGCCCCGGCTGCCCACCCTGAGGGGCAGCTC
  AAGTTCCACCCAGACACAGACGATGTACCTGTCCCAGCTCCAGCCGGTGACCAGAAGGAGGTGGACACTT
  CAGAAAAGAAACTTCTCGAGCGGCTCCCTGAGGTTGAGGTGCCCCAGCATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201786 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFRE
  KLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRMLLKAKMDAEQDPNVQVDHLNLLKQFEH
  LDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQ
  RRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPK
  NEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRF
  EEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQL
  KFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006184
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006184.6
• RefSeq Size: 2585 bp
• RefSeq ORF: 1386 bp
• Locus ID: 4924
• UniProt ID: Q02818
• Cytogenetics: 19q13.33
• Domains: EFh
• MW: 53.9 kDa
• Gene Summary: This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]