Transmembrane Protein 175 (TMEM175) (NM_032326) Human Tagged ORF Clone

CAT#: RC201422

TMEM175 (Myc-DDK-tagged)-Human transmembrane protein 175 (TMEM175)

Product Images

Western blot validation of overexpression lysate (Cat# LY410202) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201422 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM175
• Synonyms: hTMEM175
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201422 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGCCCCGGACCCCAGAGCAGGCACTGGATACACCGGGGGACTGCCCCCCAGGCAGGAGAGACG
  AGGACGCTGGGGAGGGGATCCAGTGCTCCCAACGCATGCTCAGCTTCAGTGACGCCCTGCTGTCCATCAT
  CGCCACCGTCATGATCCTGCCTGTGACCCACACGGAGATCTCCCCAGAACAGCAGTTCGACAGAAGTGTA
  CAGAGGCTTCTGGCAACACGGATTGCCGTCTACCTGATGACCTTTCTCATCGTGACAGTGGCCTGGGCAG
  CACACACAAGGTTGTTCCAAGTTGTTGGGAAAACAGACGACACACTTGCCCTGCTCAACCTGGCCTGCAT
  GATGACCATCACCTTCCTGCCTTACACGTTTTCGTTAATGGTGACCTTCCCTGATGTGCCTCTGGGCATC
  TTCTTGTTCTGTGTGTGTGTGATCGCCATCGGGGTCGTGCAGGCACTGATTGTGGGGTACGCATTCCACT
  TCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTGTACCGACGACACGTCCTGGG
  CATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCCATCTTCTCTCTCTTCTTTGTCCCCTTGTCT
  TACCTGCTGATGGTGACTGTCATCCTCCTCCCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAGGC
  TCCTGGGCCACAGGGAGCCCTCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTCAG
  CAAGGAGCGCGTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACATC
  TGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTGGCCGCCCTGA
  GTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTGGGACTGCTGTGGTTCGCCCA
  CCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCCATGGGGCTGCTGAACACGCTCTCGCTGGCC
  TTCGTGGGTGGCCTCCCACTAGCCTACCAGCAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCTGG
  AGCGCGTGCGTGTCAGCTGCACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACGGC
  GCTGCTGCACCAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGTTC
  GCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTGAGCAGGTTCA
  GTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTGTTGCTGCGCCTGCTCGTGGG
  CCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCCCGGCCCGAACACCCCCCGCCAGCCCCCACG
  GGCCAGGACGACCCACAGTCCCAGCTCCTCCCTGCCCCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201422 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQPRTPEQALDTPGDCPPGRRDEDAGEGIQCSQRMLSFSDALLSIIATVMILPVTHTEISPEQQFDRSV
  QRLLATRIAVYLMTFLIVTVAWAAHTRLFQVVGKTDDTLALLNLACMMTITFLPYTFSLMVTFPDVPLGI
  FLFCVCVIAIGVVQALIVGYAFHFPHLLSPQIQRSAHRALYRRHVLGIVLQGPALCFAAAIFSLFFVPLS
  YLLMVTVILLPYVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDI
  CEDNVPDPKDVKERFSGSLVAALSATGPRFLAYFGSFATVGLLWFAHHSLFLHVRKATRAMGLLNTLSLA
  FVGGLPLAYQQTSAFARQPRDELERVRVSCTIIFLASIFQLAMWTTALLHQAETLQPSVWFGGREHVLMF
  AKLALYPCASLLAFASTCLLSRFSVGIFHLMQIAVPCAFLLLRLLVGLALATLRVLRGLARPEHPPPAPT
  GQDDPQSQLLPAPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032326
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032326.2, NP_115702.1
• RefSeq Size: 1884 bp
• RefSeq ORF: 1515 bp
• Locus ID: 84286
• UniProt ID: Q9BSA9
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• MW: 55.6 kDa
• Gene Summary: Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[UniProtKB/Swiss-Prot Function]