Lipoamide Dehydrogenase (DLD) (NM_000108) Human Tagged Lenti ORF Clone
CAT#: RC200639L1
- LentiORF®
Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), Myc-DDK-tagged
"NM_000108" in other vectors (6)
USD 447.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Lipoamide Dehydrogenase |
Synonyms | DLDD; DLDH; E3; GCSL; LAD; OGDC-E3; PHE3 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200639).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000108 |
ORF Size | 1527 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000108.3 |
RefSeq Size | 3613 bp |
RefSeq ORF | 1530 bp |
Locus ID | 1738 |
UniProt ID | P09622 |
Cytogenetics | 7q31.1 |
Domains | pyr_redox, pyr_redox_dim |
Protein Families | Druggable Genome |
Protein Pathways | Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation |
MW | 54.2 kDa |
Gene Summary | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200639 | DLD (Myc-DDK-tagged)-Human dihydrolipoamide dehydrogenase (DLD) |
USD 474.00 |
|
RC200639L2 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), mGFP tagged |
USD 774.00 |
|
RC200639L3 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), Myc-DDK-tagged |
USD 774.00 |
|
RC200639L4 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), mGFP tagged |
USD 774.00 |
|
RG200639 | DLD (tGFP-tagged) - Human dihydrolipoamide dehydrogenase (DLD) |
USD 674.00 |
|
SC126940 | DLD (untagged)-Human dihydrolipoamide dehydrogenase (DLD) |
USD 475.00 |
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