IGBP1 (NM_001551) Human Tagged ORF Clone

CAT#: RC200387

IGBP1 (Myc-DDK-tagged)-Human immunoglobulin (CD79A) binding protein 1 (IGBP1)

Product Images

Circular map for RC200387

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  Western blot validation of overexpression lysate (Cat# LY400594) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200387 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IGBP1 protein (Cat# TP300387). The protein was produced from HEK293T cells transfected with IGBP1 cDNA clone (Cat# RC200387) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IGBP1
• Synonyms: ALPHA-4; alpha4; IBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200387 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGAGGACGAGTTACAGCTGCCGCGGCTCCCCGAGCTGTTCGAAACTGGTAGACAGTTACTGG
  ACGAAGTAGAAGTGGCGACTGAACCCGCCGGTTCCCGGATAGTCCAGGAGAAGGTGTTCAAGGGCTTGGA
  CCTCCTTGAGAAGGCTGCCGAAATGTTATCGCAGCTCGACTTGTTCAGCCGAAATGAAGATTTGGAAGAG
  ATTGCTTCCACCGACCTGAAGTACCTTTTGGTGCCAGCGTTTCAAGGAGCCCTCACCATGAAACAAGTCA
  ACCCCAGCAAGCGTCTAGATCATTTGCAGCGGGCTCGAGAACACTTTATAAACTACTTAACTCAGTGCCA
  TTGCTATCATGTGGCAGAGTTTGAGCTGCCCAAAACCATGAACAACTCTGCTGAAAATCACACTGCCAAT
  TCCTCCATGGCTTATCCTAGTCTCGTTGCTATGGCATCTCAAAGACAGGCTAAAATACAGAGATACAAGC
  AGAAGAAGGAGTTGGAGCATAGGTTGTCTGCAATGAAATCTGCTGTGGAAAGTGGTCAAGCAGATGATGA
  GCGTGTTCGTGAATATTATCTTCTTCACCTTCAGAGGTGGATTGATATCAGCTTAGAAGAGATTGAGAGC
  ATTGACCAGGAAATAAAGATCCTGAGAGAAAGAGACTCTTCAAGAGAGGCATCAACTTCTAACTCATCTC
  GCCAGGAGAGGCCTCCAGTGAAACCCTTCATTCTCACTCGGAACATGGCTCAAGCCAAAGTATTTGGAGC
  TGGTTATCCAAGTCTGCCAACTATGACGGTGAGTGACTGGTATGAGCAACATCGGAAATATGGAGCATTA
  CCGGATCAGGGAATAGCCAAGGCAGCACCAGAGGAATTCAGAAAAGCAGCTCAGCAACAGGAAGAACAAG
  AAGAAAAGGAGGAAGAGGATGATGAACAAACACTCCACAGAGCCCGGGAGTGGGATGACTGGAAGGACAC
  CCATCCTAGGGGCTATGGGAACCGACAGAACATGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200387 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEDELQLPRLPELFETGRQLLDEVEVATEPAGSRIVQEKVFKGLDLLEKAAEMLSQLDLFSRNEDLEE
  IASTDLKYLLVPAFQGALTMKQVNPSKRLDHLQRAREHFINYLTQCHCYHVAEFELPKTMNNSAENHTAN
  SSMAYPSLVAMASQRQAKIQRYKQKKELEHRLSAMKSAVESGQADDERVREYYLLHLQRWIDISLEEIES
  IDQEIKILRERDSSREASTSNSSRQERPPVKPFILTRNMAQAKVFGAGYPSLPTMTVSDWYEQHRKYGAL
  PDQGIAKAAPEEFRKAAQQQEEQEEKEEEDDEQTLHRAREWDDWKDTHPRGYGNRQNMG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001551
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001551.3
• RefSeq Size: 1679 bp
• RefSeq ORF: 1020 bp
• Locus ID: 3476
• UniProt ID: P78318
• Cytogenetics: Xq13.1
• Domains: TAP42
• Protein Families: Druggable Genome
• MW: 39.2 kDa
• Gene Summary: The proliferation and differentiation of B cells is dependent upon a B-cell antigen receptor (BCR) complex. Binding of antigens to specific B-cell receptors results in a tyrosine phosphorylation reaction through the BCR complex and leads to multiple signal transduction pathways. [provided by RefSeq, Jul 2008]