Slc2a1 (NM_011400) Mouse Tagged ORF Clone

CAT#: MR207871

Slc2a1 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 1 (Slc2a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc2a1
• Synonyms: Glut-1; Glut1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207871 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCAGCAGCAAGAAGGTGACGGGCCGCCTCATGTTGGCTGTGGGAGGAGCAGTGCTCGGATCAC
  TGCAGTTCGGCTATAACACTGGTGTCATCAACGCCCCCCAGAAGGTTATTGAGGAGTTCTACAATCAAAC
  ATGGAACCACCGCTACGGAGAGCCCATCCCATCCACCACACTCACCACGCTTTGGTCTCTCTCCGTGGCC
  ATCTTCTCTGTCGGGGGCATGATTGGTTCCTTCTCTGTCGGCCTCTTTGTTAATCGCTTTGGCAGGCGGA
  ACTCCATGCTGATGATGAACCTGTTGGCCTTTGTGGCTGCTGTGCTTATGGGCTTCTCCAAACTGGGCAA
  GTCCTTTGAGATGCTGATCCTGGGCCGCTTCATCATCGGTGTGTACTGCGGCCTGACTACTGGCTTTGTG
  CCCATGTATGTGGGAGAGGTGTCACCTACAGCTCTACGTGGAGCCCTAGGCACACTGCACCAGCTGGGAA
  TCGTCGTTGGCATCCTTATTGCCCAGGTGTTTGGCTTAGACTCCATCATGGGCAATGCAGACTTGTGGCC
  TCTGCTGCTCAGTGTCATCTTCATCCCAGCCCTGCTACAGTGTATCCTGTTGCCCTTCTGCCCCGAGAGC
  CCCCGCTTCCTGCTCATCAATCGTAACGAGGAGAACCGGGCCAAGAGTGTGCTGAAGAAGCTTCGAGGGA
  CAGCCGATGTGACCCGAGACCTGCAGGAGATGAAAGAAGAGGGTCGGCAGATGATGCGGGAGAAGAAGGT
  CACCATCTTGGAGCTGTTCCGCTCACCCGCCTACCGCCAGCCCATCCTCATCGCTGTGGTGCTGCAGCTG
  TCCCAGCAGCTGTCGGGTATCAATGCTGTGTTCTACTACTCAACGAGCATCTTCGAGAAGGCAGGTGTGC
  AGCAGCCTGTGTACGCCACCATCGGCTCCGGTATCGTCAACACGGCCTTCACTGTGGTGTCGCTGTTTGT
  TGTAGAGCGAGCTGGACGACGGACCCTGCACCTCATTGGCCTGGCTGGCATGGCAGGCTGTGCTGTGCTC
  ATGACCATCGCCCTGGCCTTGCTGGAACGGCTGCCTTGGATGTCCTATCTGAGCATCGTGGCCATCTTTG
  GCTTTGTGGCCTTCTTTGAAGTAGGCCCTGGTCCTATTCCATGGTTCATTGTGGCCGAGCTGTTCAGCCA
  GGGGCCCCGTCCTGCTGCTATTGCTGTGGCTGGCTTCTCCAACTGGACCTCAAACTTCATTGTGGGCATG
  TGCTTCCAGTATGTGGAGCAACTGTGCGGCCCCTACGTCTTCATCATCTTCACGGTGCTCCTCGTGCTCT
  TCTTCATCTTCACCTACTTCAAAGTCCCTGAGACCAAAGGCCGAACCTTCGATGAGATCGCTTCCGGCTT
  CCGGCAGGGGGGTGCCAGCCAAAGTGACAAGACACCCGAGGAGCTCTTCCACCCTCTGGGGGCGGACTCC
  CAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207871 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPSSKKVTGRLMLAVGGAVLGSLQFGYNTGVINAPQKVIEEFYNQTWNHRYGEPIPSTTLTTLWSLSVA
  IFSVGGMIGSFSVGLFVNRFGRRNSMLMMNLLAFVAAVLMGFSKLGKSFEMLILGRFIIGVYCGLTTGFV
  PMYVGEVSPTALRGALGTLHQLGIVVGILIAQVFGLDSIMGNADLWPLLLSVIFIPALLQCILLPFCPES
  PRFLLINRNEENRAKSVLKKLRGTADVTRDLQEMKEEGRQMMREKKVTILELFRSPAYRQPILIAVVLQL
  SQQLSGINAVFYYSTSIFEKAGVQQPVYATIGSGIVNTAFTVVSLFVVERAGRRTLHLIGLAGMAGCAVL
  MTIALALLERLPWMSYLSIVAIFGFVAFFEVGPGPIPWFIVAELFSQGPRPAAIAVAGFSNWTSNFIVGM
  CFQYVEQLCGPYVFIIFTVLLVLFFIFTYFKVPETKGRTFDEIASGFRQGGASQSDKTPEELFHPLGADS
  QV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011400
• ORF Size: 1479 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011400.3
• RefSeq Size: 2573 bp
• RefSeq ORF: 1479 bp
• Locus ID: 20525
• UniProt ID: P17809
• Cytogenetics: 4
• MW: 54 kDa
• Gene Summary: Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:17320047). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (By similarity). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (By similarity).[UniProtKB/Swiss-Prot Function]