Puf60 (NM_133691) Mouse Tagged ORF Clone
CAT#: MG218937
- TrueORF®
Puf60 (tGFP-tagged) - Mouse poly-U binding splicing factor 60 (Puf60) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_133691" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Puf60 |
Synonyms | 2410104I19Rik; 2810454F19Rik; SIAHBP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218937 representing NM_133691
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGCGACTATAGCTCTCCAGGTCAATGGCCAACAAGGAGGAGGGTCGGAGCCAGCAGCAGCGG CTGCAGCGGCGGCGGCGGCAGTGGTGGCAGCAGGAGACAAATGGAAACCTCCACAGGGCACAGAATCCAT CAAGATGGAAAATGGACAAAGCACAGGCACCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAG GCCCTCCAGAAGGCCAAGAAATATGCAATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAACAGACCATCG CGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCAGCTCAACGGCAGCGGGCACTGGCTATCATGTG CCGGGTCTATGTGGGCTCCATCTACTATGAGCTGGGAGAAGACACTATTCGCCAGGCCTTTGCTCCCTTT GGCCCCATCAAGAGCATTGATATGTCCTGGGACTCCGTTACCATGAAGCATAAGGGCTTTGCCTTCGTGG AGTACGAGGTCCCAGAAGCTGCGCAGCTCGCTTTGGAGCAGATGAACTCTGTGATGCTTGGGGGCAGGAA CATCAAGGTGGGAAGACCCAGCAACATTGGACAAGCCCAACCCATCATAGATCAGCTGGCTGAGGAAGCT AGGGCTTTCAACCGAATATATGTGGCCTCTGTACATCAAGACCTGTCTGATGATGACATCAAGAGTGTAT TTGAAGCCTTTGGCAAGATCAAGTCTTGTACGCTGGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTA TGGTTTTATCGAATATGAGAAGGCCCAGTCGTCCCAGGATGCTGTGTCCTCCATGAACCTCTTTGATCTG GGTGGCCAGTACTTGAGGGTGGGCAAGGCCGTCACACCCCCCATGCCCCTGCTAACACCTGCCACGCCTG GAGGTCTCCCGCCTGCTGCTGCTGTGGCCGCAGCTGCAGCCACGGCCAAGATTACAGCTCAGGAAGCAGT GGCTGGAGCAGCAGTGCTGGGTACTTTAGCCACACCAGGACTAGTGTCCCCAGCACTGACACTGGCCCAG CCCTTAGGGGCCCTACCCCAGGCTGTCATGGCTGCCCAGGCCCCTGGAGTCATCACAGGTGTGACACCAG CCCGCCCTCCTATTCCGGTCACCATCCCCTCTGTGGGAGTAGTGAACCCCATTCTCGCCAGCCCGCCAAC GTTGGGTCTGTTGGAGCCCAAGAAGGAGAAGGAAGAGGAGGAGCTGTTTCCTGAGTCAGAACGGCCAGAG ATGTTGAGTGAGCAGGAGCACATGAGCATCTCGGGCAGCAGTGCTCGCCACATGGTCATGCAAAAGCTAC TCAGAAAACAGGAGTCTACAGTGATGGTTCTCCGAAACATGGTGGACCCCAAGGACATCGATGACGACCT GGAGGGAGAGGTGACAGAGGAGTGTGGCAAATTTGGTGCTGTGAACCGGGTCATTATCTATCAAGAAAAA CAGGGCGAAGAGGAAGATGCAGAAATCATCGTCAAGATTTTTGTGGAATTCTCCATGGCCTCAGAGACTC ACAAGGCCATCCAGGCCCTCAATGGGCGCTGGTTTGGTGGTCGCAAGGTGGTGGCTGAAGTGTATGACCA GGAGCGTTTTGATAACAGCGACCTCTCCGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218937 representing NM_133691
Red=Cloning site Green=Tags(s) MATATIALQVNGQQGGGSEPAAAAAAAAAAVVAAGDKWKPPQGTESIKMENGQSTGTKLGLPPLTPEQQE ALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPF GPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEA RAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDL GGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLATPGLVSPALTLAQ PLGALPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPE MLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEK QGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDNSDLSA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133691 |
ORF Size | 1641 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133691.5, NP_598452.2 |
RefSeq Size | 1878 bp |
RefSeq ORF | 1644 bp |
Locus ID | 67959 |
UniProt ID | Q3UEB3 |
Cytogenetics | 15 D3 |
Gene Summary | DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218836 | Puf60 (untagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2, (10ug) |
USD 561.00 |
|
MR218937 | Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2 |
USD 560.00 |
|
MR218937L3 | Lenti ORF clone of Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2 |
USD 860.00 |
|
MR218937L4 | Lenti ORF clone of Puf60 (mGFP-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 2 |
USD 860.00 |
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