Usp10 (NM_009462) Mouse Tagged ORF Clone

CAT#: MG210686

Usp10 (tGFP-tagged) - Mouse ubiquitin specific peptidase 10 (Usp10)

Product Images

Circular map for MG210686

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Usp10
• Synonyms: 2610014N07Rik; mKIAA0190; UBPO; Uchrp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210686 representing NM_009462
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCACAACCCACAGTATATCTTTGGCGATTTCAGCCCTGATGAATTCAATCAGTTTTTTGTGA
  CTCCCCGCTCTTCTGTTGAGCTCCCTCCATACAGTGGGACTCTGTGTAGCATACAGGCTGAAGATGAACT
  GCCAGATGGACAGGAGCACCAGAGGATCGAGTTTGGTGTAGATGAAGTCATCGAACCTAGTGAGGGGCTG
  CCGCGAACCCCTAGCTACAGTATTTCAAGCACCTTGAACCCCCAGGCACCCGAATTTATCCTTGGTTGTA
  CGACTTCTAAAAAGATCCCTGATGCCGTTGAAAAAGATGAGACCTACAGCTCCATTGACCAGTACCCCGC
  CTCGGCCTTGGCTCTGGAAAGCAACTCTAACGCAGAGGCTGAGACCCTGGAGAACGACAGTGGTGCCGGC
  GGCCTTGGTCAGAGGGAACGGAAAAAGAAGAAGAAGCGGCCCCCTGGGTACTACAGTTATTTGAAAGATG
  GCGGTGAGGACAGTGCTTCCCCAGCCACCCTGGTCAACGGCCATGCCACCTCAGTGGGCACAAGCGGCGA
  GGCTGTGGAGGACGCCGAGTTCATGGACGTGCTTCCTCCAGTCATGCCCAGGACTTGTGACAGCCCTCAG
  AATCCTGTGGACTTCATCAGTGGCCCTGTCCCTGACAGTCCGTTCCCCAGGACACTAGGAGGCGATGCCA
  GGACTGCAGGGCTGTGTGAGGGCTGCCATGAAGCTGACTTTGAGCAGCCCTGCCTCCCTGCAGACAGCCT
  GCTAAGGACAGCTGGGACGCAGCCCTACGTTGGCACCGATACTACTGAGAACTTCGCAGTTGCTAATGGA
  AAAATACTTGAATCCCCGGGCGAGGACACTGCTGCCAGTGGGGCAGAACTGCACACCGACGAGGGTGCAG
  ACCTGGACCCTGCGAAGCCGGAGAGCCAGTCACCTCCTGCCGAAAGTGCGCTCTCTGCCTCTGGCGCCAT
  CCCCATTAGCCAGCCTGCAAAGTCCTGGGCTAGTCTCTTTCATGATTCTAAGCCCTCTTCCTCCTCACCC
  ATGGCGTATGTGGAAACTAAGTGTTCCCCCCCTGTCCCGTCCCCCCTGGCCTCTGAAAAACAGATGGAAG
  TCAAAGAAGGGCTTGTTCCAGTGTCAGAGGATCCTGTAGCCATAAAGATTGCAGAGTTATTGGAGACTGT
  AACCCTAATACATAAGCCAGTGTCATTGCAACCCCGTGGGCTGATCAATAAAGGAAACTGGTGCTACATT
  AATGCCACCCTGCAGGCATTGGTGGCTTGCCCTCCGATGTATCACCTGATGAAGTTCATTCCTCTGTACT
  CAAAAGTGCAGCGGCCTTGCACATCAACGCCCATGATAGATAGCTTTGTTCGGCTCATGAATGAGTTCAC
  TAACATGCCAGTGCCTCCCAAACCCCGCCAAGCTCTCGGGGATAAAATCGTGAGAGATATCCGCCCAGGG
  GCTGCCTTTGAACCCACATACATATACAGACTCCTGACAGTCATCAAGTCGAGCCTGTCTGAAAAGGGCC
  GGCAGGAGGACGCAGAGGAGTATCTAGGTTTCATCCTCAATGGACTCCATGAAGAGATGCTGAGCCTGAA
  AAAGCTACTCTCACCCACGCACGAAAAACACTCTGTTTCCAATGGGCCCAGAAGCGACTTGATAGAGGAC
  GAGGAGCTGGAAGACACGGGCAAGGGCAGCGAGGACGAGTGGGAGCAAGTGGGTCCCAAGAATAAGACGT
  CCATCACCCGGCAGGCGGACTTCGTCCAGACGCCCATCACTGGCATTTTTGGGGGACACATCAGGTCTGT
  GGTCTACCAGCAGAGTTCCAAGGAGTCAGCCACTCTGCAACTGTTTTTCACGCTGCAGTTGGACATCCAG
  TCTGACAAGATACGCACAGTCCAGGATGCGCTGGAGAGCTTGGTGGCCAGGGAGTCTGTCCAAGGTTACA
  CCACCAAAACCAAGCAGGAGGTTGAGGTGAGCCGCAGAGTGACTCTGGAGAAGCTGCCCCCTGTCCTCGT
  GCTCCACCTGAAGCGCTTTGTCTACGAGAAGACAGGCGGATGCCAGAAGCTGGTCAAGAACATTGACTAC
  CCTGTGGACTTGGAGATCAGCAGAGAACTACTTTCTCCAGGCATCAAAAATAAAAATTTTAAATGCCAGA
  GAACCTATAGGCTGTTTGCAGTGGTCTACCACCATGGCAACAGCGCTACAGGCGGCCACTACACTACGGA
  CGTCTTCCAGATTGGGCTTAATGGCTGGCTACGAATCGATGACCAAACGGTCAAGGTTATTAACCAGTAC
  CAGGTGGTGAAACCGCCTGCCGACCGCACAGCCTACCTCCTATATTACCGCCGCGTGGACCTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210686 representing NM_009462
  Red=Cloning site Green=Tags(s)
  
  MALHNPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTLCSIQAEDELPDGQEHQRIEFGVDEVIEPSEGL
  PRTPSYSISSTLNPQAPEFILGCTTSKKIPDAVEKDETYSSIDQYPASALALESNSNAEAETLENDSGAG
  GLGQRERKKKKKRPPGYYSYLKDGGEDSASPATLVNGHATSVGTSGEAVEDAEFMDVLPPVMPRTCDSPQ
  NPVDFISGPVPDSPFPRTLGGDARTAGLCEGCHEADFEQPCLPADSLLRTAGTQPYVGTDTTENFAVANG
  KILESPGEDTAASGAELHTDEGADLDPAKPESQSPPAESALSASGAIPISQPAKSWASLFHDSKPSSSSP
  MAYVETKCSPPVPSPLASEKQMEVKEGLVPVSEDPVAIKIAELLETVTLIHKPVSLQPRGLINKGNWCYI
  NATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPG
  AAFEPTYIYRLLTVIKSSLSEKGRQEDAEEYLGFILNGLHEEMLSLKKLLSPTHEKHSVSNGPRSDLIED
  EELEDTGKGSEDEWEQVGPKNKTSITRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQLFFTLQLDIQ
  SDKIRTVQDALESLVARESVQGYTTKTKQEVEVSRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLVKNIDY
  PVDLEISRELLSPGIKNKNFKCQRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQY
  QVVKPPADRTAYLLYYRRVDLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009462
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009462.1, NP_033488.1
• RefSeq Size: 3236 bp
• RefSeq ORF: 2382 bp
• Locus ID: 22224
• UniProt ID: P52479
• Cytogenetics: 8 E1
• Gene Summary: Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.[UniProtKB/Swiss-Prot Function]