Usp10 (NM_009462) Mouse Tagged ORF Clone

CAT#: MR210686

Usp10 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 10 (Usp10)

Product Images

Circular map for MR210686

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp10
• Synonyms: 2610014N07Rik; mKIAA0190; UBPO; Uchrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210686 representing NM_009462
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCACAACCCACAGTATATCTTTGGCGATTTCAGCCCTGATGAATTCAATCAGTTTTTTGTGA
  CTCCCCGCTCTTCTGTTGAGCTCCCTCCATACAGTGGGACTCTGTGTAGCATACAGGCTGAAGATGAACT
  GCCAGATGCAGGACAGGAGCACCAGAGGATCGAGTTTGGTGTAGATGAAGTCATCGAACCTAGTGAGGGG
  CTGCCGCCAACTCCTAGCTACAGTATTTCAAGCACCTTGAACCCCCAGGCACCCGAATTTATCCTTGGTT
  GTACGACTTCTAAAAAGATCCCTGAAGCCGTTGAAAAAGATGAGACCTACAGCTCCATTGACCAGTACCC
  CGCCTCGGCCTTGGCTCTGGAAAGCAACTCTAACGCAGAGGCTGAGACCCTGGAGAACGACAGTGGTGCC
  GGCGGCCTTGGTCAGAGGGAACGGAAAAAGAAGAAGAAGCGGCCCCCTGGGTACTACAGTTATTTGAAAG
  ATGGCGGTGAGGACAGTGCTTCCCCAGCCACCCTGGTCAACGGCCATGCCACCTCAGTGGGCACGAGCGG
  CGAGGCTGTGGAGGACGCCGAGTTCATGGACGTGCTTCCTCCAGTCATGCCCAGGACTTGTGACAGCCCT
  CAGAATCCTGTGGACTTCATCAGTGGCCCTGTCCCTGACAGTCCGTTCCCCAGGACACTAGGAGGCGATG
  CCAGGACTGCAGGGCTGTGTGAGGGCTGCCATGAAGCTGACTTTGAGCAGCCCTGCCTCCCTGCAGACAG
  CCTGCTAAGGACAGCTGGGACGCAGCCCTACGTTGGCACCGATACTACTGAGAACTTCGCAGTTGCTAAT
  GGGAAAATACTTGAATCCCCGGGCGAGGACACTGCTGCCAACGGGGCAGAACTGCACACCGACGAGGGTG
  CAGACCTGGACCCTGCGAAGCCGGAGAGCCAGTCACCTCCTGCCGAAAGTGCGCTCTCTGCCTCTGGCGC
  CATCCCCATTAGCCAGCCTGCAAAGTCCTGGGCTAGTCTCTTTCATGATTCTAAGCCCTCTGCCTCCTCA
  CCCATGGCGTATGTGGAAACTAAGTGTTCCCCCCCTGTCCCATCCCCCCTGGCCTCTGAAAAACAGATGG
  AAGTCAAAGAAGGGCTTGTTCCAGTGTCAGAGGATCCTGTAGCCATAAAGATTGCAGAGTTATTGGAGAC
  TGTAACCCTAATACATAAGCCAGTGTCATTGCAACCCCGTGGGCTGATCAATAAAGGAAACTGGTGCTAC
  ATTAATGCCACCCTGCAGGCATTGGTGGCTTGCCCTCCGATGTATCACCTGATGAAGTTCATTCCTCTGT
  ACTCAAAAGTGCAGCGGCCTTGCACATCAACGCCCATGATAGATAGCTTTGTTCGGCTCATGAATGAGTT
  CACTAACATGCCAGTGCCTCCCAAACCCCGCCAAGCTCTCGGGGATAAAATCGTGAGAGATATCCGCCCA
  GGGGCTGCCTTTGAACCCACATACATATACAGACTCCTGACAGTCATCAAGTCGAGCCTGTCTGAAAAGG
  GCCGGCAGGAGGACGCAGAGGAGTATCTAGGTTTCATCCTCAATGGACTCCATGAAGAGATGCTGAGCCT
  GAAGAAGCTACTCTCACCCACGCACGAAAAACACTCTGTTTCCAATGGGCCCAGAAGCGACTTGATAGAG
  GACGAGGAGCTGGAAGACACGGGCAAGGGCAGCGAGGACGAGTGGGAGCAAGTGGGTCCCAAGAATAAGA
  CGTCCATCACCCGGCAGGCGGACTTCGTCCAGACGCCCATCACTGGCATTTTTGGGGGACACATCAGGTC
  TGTGGTCTACCAGCAGAGTTCCAAAGAGTCAGCTACTCTGCAACTGTTTTTCACGCTGCAGTTGGACATC
  CAGTCTGACAAGATACGCACAGTCCAGGATGCGCTGGAGAGCTTGGTGGCCAGGGAGTCTGTCCAAGGTT
  ACACCACCAAAACCAAGCAGGAGGTTGAGGTGAGCCGCAGAGTGACTCTGGAGAAGCTGCCCCCTGTCCT
  CGTGCTCCACCTGAAGCGCTTTGTCTATGAGAAGACAGGTGGATGCCAGAAGCTGGTCAAGAACATTGAC
  TACCCTGTGGACTTGGAGATCAGCAGAGAACTACTTTCTCCAGGCATCAAAAATAAAAATTTTAAATGCC
  AGAGAACCTATAGGCTGTTTGCAGTGGTCTACCACCATGGCAACAGCGCTACAGGCGGCCACTACACTAC
  GGACGTCTTCCAGATTGGGCTTAATGGCTGGCTACGAATCGATGACCAAACGGTCAAGGTTATTAACCAG
  TACCAGGTGGTGAAACCGCCTGCCGACCGCACAGCCTACCTCCTATATTACCGCCGCGTGGACCTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210686 representing NM_009462
  Red=Cloning site Green=Tags(s)
  
  MALHNPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTLCSIQAEDELPDAGQEHQRIEFGVDEVIEPSEG
  LPPTPSYSISSTLNPQAPEFILGCTTSKKIPEAVEKDETYSSIDQYPASALALESNSNAEAETLENDSGA
  GGLGQRERKKKKKRPPGYYSYLKDGGEDSASPATLVNGHATSVGTSGEAVEDAEFMDVLPPVMPRTCDSP
  QNPVDFISGPVPDSPFPRTLGGDARTAGLCEGCHEADFEQPCLPADSLLRTAGTQPYVGTDTTENFAVAN
  GKILESPGEDTAANGAELHTDEGADLDPAKPESQSPPAESALSASGAIPISQPAKSWASLFHDSKPSASS
  PMAYVETKCSPPVPSPLASEKQMEVKEGLVPVSEDPVAIKIAELLETVTLIHKPVSLQPRGLINKGNWCY
  INATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRP
  GAAFEPTYIYRLLTVIKSSLSEKGRQEDAEEYLGFILNGLHEEMLSLKKLLSPTHEKHSVSNGPRSDLIE
  DEELEDTGKGSEDEWEQVGPKNKTSITRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQLFFTLQLDI
  QSDKIRTVQDALESLVARESVQGYTTKTKQEVEVSRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLVKNID
  YPVDLEISRELLSPGIKNKNFKCQRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQ
  YQVVKPPADRTAYLLYYRRVDLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009462
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009462.2
• RefSeq Size: 3236 bp
• RefSeq ORF: 2382 bp
• Locus ID: 22224
• UniProt ID: P52479
• Cytogenetics: 8 E1
• MW: 87.5 kDa
• Gene Summary: Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.[UniProtKB/Swiss-Prot Function]