Neurod4 (NM_007501) Mouse Tagged ORF Clone
CAT#: MG204851
- TrueORF®
Neurod4 (tGFP-tagged) - Mouse neurogenic differentiation 4 (Neurod4)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_007501" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Neurod4 |
Synonyms | AI846749; ATH-3; Ato; Atoh3; bHLH; bHLHa4; Math; MATH-; MATH-3; Math3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204851 representing NM_007501
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAAATGTATATGAAATCCAAGGACATGGTGGAGCTGGTCAACACACAATCCTGGATGGACAAAG GTCTGAGCTCTCAAAATGAGATGAAGGAGCAAGAGAGAAGACCGGGCTCTTATGGAATGCTCGGAACCTT AACTGAAGAGCATGACAGTATTGAGGAGGATGAAGAAGAGGAAGAAGATGGAGATAAACCTAAAAGAAGA GGTCCCAAGAAAAAGAAGATGACTAAAGCTCGCCTTGAAAGATTCAGGGCTCGAAGAGTCAAGGCCAATG CTAGAGAACGGACCCGGATGCATGGCCTGAATGATGCCTTGGATAATCTTAGGAGAGTCATGCCATGTTA CTCTAAAACTCAAAAGCTTTCCAAGATAGAGACTCTTCGACTGGCAAGGAACTACATCTGGGCCTTGTCT GAAGTCCTGGAGACTGGTCAGACACTTGAAGGGAAGGGATTTGTAGAGATGCTATGTAAAGGTCTCTCTC AACCCACAAGCAACCTGGTTGCTGGATGCCTCCAACTGGGGCCTCAATCTACCCTCCTGGAGAAGCATGA GGAAAAATCTTCAATTTGTGACTCTACTATCTCTGTCCACAGCTTCAACTATCAGTCTCCAGGGCTCCCC AGCCCTCCTTATGGCCATATGGAAACACATTCTCTCCATCTCAAGCCTCAACCATTTAAGAGTTTGGGTG ACTCTTTTGGGAGCCATCCACCTGACTGCAGTACCCCCCCTTATGAGGGTCCACTCACACCACCCCTGAG CATTAGTGGCAACTTCTCCTTAAAGCAAGACGGCTCCCCTGATTTGGAAAAATCCTACAATTTCATGCCA CATTATACCTCTGCAAGTCTAAGTTCAGGGCATGTGCATTCAACTCCCTTTCAGACTGGCACTCCCCGCT ATGATGTTCCTGTAGACCTGAGCTATGATTCCTACTCCCACCATAGCATTGGAACTCAGCTCAATACGAT CTTCTCTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204851 representing NM_007501
Red=Cloning site Green=Tags(s) MAKMYMKSKDMVELVNTQSWMDKGLSSQNEMKEQERRPGSYGMLGTLTEEHDSIEEDEEEEEDGDKPKRR GPKKKKMTKARLERFRARRVKANARERTRMHGLNDALDNLRRVMPCYSKTQKLSKIETLRLARNYIWALS EVLETGQTLEGKGFVEMLCKGLSQPTSNLVAGCLQLGPQSTLLEKHEEKSSICDSTISVHSFNYQSPGLP SPPYGHMETHSLHLKPQPFKSLGDSFGSHPPDCSTPPYEGPLTPPLSISGNFSLKQDGSPDLEKSYNFMP HYTSASLSSGHVHSTPFQTGTPRYDVPVDLSYDSYSHHSIGTQLNTIFSD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007501 |
ORF Size | 990 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007501.5 |
RefSeq Size | 3376 bp |
RefSeq ORF | 993 bp |
Locus ID | 11923 |
UniProt ID | O09105 |
Cytogenetics | 10 77.81 cM |
Gene Summary | This gene belongs to the neurogenic differentiation factor family and encodes a basic helix-loop-helix (bHLH) transcription factor which is expressed in the developing nervous system with high levels of expression in the brain, retina and cranial ganglions. Expression gradually becomes restricted to the neural retina. It is a key gene in the Ngn2-regulated neuronal differentiation pathway, coordinating the onset of cortical gene transcription. This gene also regulates amacrine cell fate determination in the retina. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205890 | Neurod4 (untagged) - Mouse neurogenic differentiation 4 (Neurod4), (10ug) |
USD 300.00 |
|
MR204851 | Neurod4 (Myc-DDK-tagged) - Mouse neurogenic differentiation 4 (Neurod4) |
USD 300.00 |
|
MR204851L3 | Lenti ORF clone of Neurod4 (Myc-DDK-tagged) - Mouse neurogenic differentiation 4 (Neurod4) |
USD 600.00 |
|
MR204851L4 | Lenti ORF clone of Neurod4 (mGFP-tagged) - Mouse neurogenic differentiation 4 (Neurod4) |
USD 600.00 |
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