Eif2b1 (NM_145371) Mouse Tagged ORF Clone
CAT#: MG204296
- TrueORF®
Eif2b1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 1 (alpha) (Eif2b1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_145371" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Eif2b1 |
Synonyms | 26kD; D5Ertd406; D5Ertd406e; EIF2; EIF2BA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204296 representing NM_145371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGTGAGTTAATTGAATACTTTAAGTCTCAGATGAAAGGAGATCCTAAAATGGCCTCAGCTG TGGCTGCCATCCAGACTTTGTTGGAATTCTTGAAGAGAGATAAAGGAGAAACACTCCAGGGCCTGAGAGC AAATCTCACCTATGCCATAAAAACCCTCTGTGGTGTGGACTCCTCAGTGGCCGTGTCATCTGGCGGGGAG CTCTTCCTTCGCTTCATCAGCCTCACCTCCCTGGAATACTCTGACTACTCCAAATGTAAGAAGATCATGA TTGAGAGAGGAGAGCTGTTCCTGAGAAGAATATCCCTGTCGAGAAATAAGATTGCCAATCTGTGTCATAC ATTCATCAAAGACGGGGCGAGAATTTTGACTCACGCCTACTCCAGAGTTGTCTTAAGAGTCCTGGAAGAA GCCGTGGCAGCCAAGAAACGGTTCAGTGTGTATATCACGGAGTCGCAGCCTGATTTATCTGGTAAGAAAA TGGCCAAAGCCCTCTCCCACCTCAACGTCCCTGTCACTGTGGTGCTGGATGCTGCTGTCGGCTATATCAT GGAGAAAGCAGATCTTGTCATAGTTGGTGCTGAAGGAGTGGTAGAGAACGGAGGCATTATTAACAAGATT GGAACCAACCAGATGGCCGTGTGTGCCAAAGCCCAGAATAAGCCCTTCTATGTGGTTGCAGAAAGCTTCA AGTTCGTACGCCTCTTTCCACTCAACCAGGAGGACGTCCCAGATAAGTTTAAGTACAAGGCAGACACTCT GAAGTCTGTGCAGACTGGGCAGGATCTCAAAGAGGAACACCCATGGGTGGACTACACCTCCCCATCCCTC ATCACCCTGCTGTTTACGGACCTGGGTGTGTTGACGCCATCTGCTGTAAGCGATGAGCTCATCAAGCTGT ACCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204296 representing NM_145371
Red=Cloning site Green=Tags(s) MEDGELIEYFKSQMKGDPKMASAVAAIQTLLEFLKRDKGETLQGLRANLTYAIKTLCGVDSSVAVSSGGE LFLRFISLTSLEYSDYSKCKKIMIERGELFLRRISLSRNKIANLCHTFIKDGARILTHAYSRVVLRVLEE AVAAKKRFSVYITESQPDLSGKKMAKALSHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKI GTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQEDVPDKFKYKADTLKSVQTGQDLKEEHPWVDYTSPSL ITLLFTDLGVLTPSAVSDELIKLYL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_145371 |
ORF Size | 915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145371.4, NP_663346.1 |
RefSeq Size | 1938 bp |
RefSeq ORF | 918 bp |
Locus ID | 209354 |
UniProt ID | Q99LC8 |
Cytogenetics | 5 63.67 cM |
Gene Summary | This gene encodes the alpha subunit of the eukaryotic translation initiation factor complex 2B (eIF2B). The eIF2B complex is a heterodecamer comprised of two molecules each of alpha, beta, gamma, delta and epsilon subunits. The eIF2B complex is a critical regulator of protein synthesis acting as the guanine nucleotide exchange factor for eIF2 to enable the formation of ternary complex that is required for the initiation of mRNA translation. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212523 | Eif2b1 (untagged) - Mouse eukaryotic translation initiation factor 2B, subunit 1 (alpha) (Eif2b1), (10ug) |
USD 330.00 |
|
MR204296 | Eif2b1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 1 (alpha) (Eif2b1) |
USD 300.00 |
|
MR204296L3 | Lenti ORF clone of Eif2b1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 1 (alpha) (Eif2b1) |
USD 600.00 |
|
MR204296L4 | Lenti ORF clone of Eif2b1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 1 (alpha) (Eif2b1) |
USD 600.00 |
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