Eif2b1 (NM_145371) Mouse Untagged Clone

CAT#: MC212523

Eif2b1 (untagged) - Mouse eukaryotic translation initiation factor 2B, subunit 1 (alpha) (Eif2b1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif2b1
• Synonyms: 26kD; D5Ertd406; D5Ertd406e; EIF2; EIF2BA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212523 representing NM_145371
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGGTGAGTTAATTGAATACTTTAAGTCTCAGATGAAAGGAGATCCTAAAATGGCCTCAGCTG
  TGGCTGCCATCCAGACTTTGTTGGAATTCTTGAAGAGAGATAAAGGAGAAACACTCCAGGGCCTGAGAGC
  AAATCTCACCTATGCCATAAAAACCCTCTGTGGTGTGGACTCCTCAGTGGCCGTGTCATCTGGCGGGGAG
  CTCTTCCTTCGCTTCATCAGCCTCACCTCCCTGGAATACTCTGACTACTCCAAATGTAAGAAGATCATGA
  TTGAGAGAGGAGAGCTGTTCCTGAGAAGAATATCCCTGTCGAGAAATAAGATTGCCAATCTGTGTCATAC
  ATTCATCAAAGACGGGGCGAGAATTTTGACTCACGCCTACTCCAGAGTTGTCTTAAGAGTCCTGGAAGAA
  GCCGTGGCAGCCAAGAAACGGTTCAGTGTGTATATCACGGAGTCGCAGCCTGATTTATCTGGTAAGAAAA
  TGGCCAAAGCCCTCTCCCACCTCAACGTCCCTGTCACTGTGGTGCTGGATGCTGCTGTCGGCTATATCAT
  GGAGAAAGCAGATCTTGTCATAGTTGGTGCTGAAGGAGTGGTAGAGAACGGAGGCATTATTAACAAGATT
  GGAACCAACCAGATGGCCGTGTGTGCCAAAGCCCAGAATAAGCCCTTCTATGTGGTTGCAGAAAGCTTCA
  AGTTCGTACGCCTCTTTCCACTCAACCAGGAGGACGTCCCAGATAAGTTTAAGTACAAGGCAGACACTCT
  GAAGTCTGTGCAGACTGGGCAGGATCTCAAAGAGGAACACCCATGGGTGGACTACACCTCCCCATCCCTC
  ATCACCCTGCTGTTTACGGACCTGGGTGTGTTGACGCCATCTGCTGTAAGCGATGAGCTCATCAAGCTGT
  ACCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145371
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145371.4, NP_663346.1
• RefSeq Size: 2012 bp
• RefSeq ORF: 918 bp
• Locus ID: 209354
• UniProt ID: Q99LC8
• Cytogenetics: 5 63.67 cM
• Gene Summary: This gene encodes the alpha subunit of the eukaryotic translation initiation factor complex 2B (eIF2B). The eIF2B complex is a heterodecamer comprised of two molecules each of alpha, beta, gamma, delta and epsilon subunits. The eIF2B complex is a critical regulator of protein synthesis acting as the guanine nucleotide exchange factor for eIF2 to enable the formation of ternary complex that is required for the initiation of mRNA translation. [provided by RefSeq, Aug 2015]