Atp6v1g1 (NM_024173) Mouse Tagged ORF Clone
CAT#: MG200546
- TrueORF®
Atp6v1g1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit G1 (Atp6v1g1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_024173" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Atp6v1g1 |
Synonyms | 1810024D14Rik; AA960677; Atp6g1; ATP6J; VAG1; Vma10 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200546 representing NM_024173
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGTCAGTCGCAGGGCATCCAGCAGCTACTGCAGGCCGAGAAGCGGGCCGCCGAGAAGGTGTCCG AGGCCCGCAAGCGAAAGAACCGGAGGCTGAAGCAGGCCAAAGAAGAAGCCCAGGCTGAAATTGAACAGTA CCGCCTGCAGAGGGAGAAGGAGTTCAAGGCCAAGGAAGCTGCGGCATTGGGCTCCCATGGCAGCTGCAGC AGTGAGGTGGAAAAGGAGACCCGGGAGAAGATGACCGTCCTCCAGAACTACTTCGAGCAGAACAGGGATG AAGTCCTGGATAACCTCTTGGCCTTTGTGTGTGACATCCGGCCGGAAATCCATGAAAACTACCGCATAAA CGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200546 representing NM_024173
Red=Cloning site Green=Tags(s) MASQSQGIQQLLQAEKRAAEKVSEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKAKEAAALGSHGSCS SEVEKETREKMTVLQNYFEQNRDEVLDNLLAFVCDIRPEIHENYRING TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_024173 |
ORF Size | 354 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024173.2, NP_077135.1 |
RefSeq Size | 1104 bp |
RefSeq ORF | 357 bp |
Locus ID | 66290 |
UniProt ID | Q9CR51 |
Cytogenetics | 4 B3 |
Gene Summary | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210320 | Atp6v1g1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit G1 (Atp6v1g1), (10ug) |
USD 165.00 |
|
MR200546 | Atp6v1g1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit G1 (Atp6v1g1) |
USD 150.00 |
|
MR200546L3 | Lenti ORF clone of Atp6v1g1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit G1 (Atp6v1g1) |
USD 450.00 |
|
MR200546L4 | Lenti ORF clone of Atp6v1g1 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit G1 (Atp6v1g1) |
USD 450.00 |
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