Snapc5 (BC028529) Mouse Tagged ORF Clone

CAT#: MG200288

Snapc5 (tGFP-tagged) - Mouse small nuclear RNA activating complex, polypeptide 5 (cDNA clone MGC:41384 IMAGE:1381169)

Product Images

Circular map for MG200288

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snapc5
• Synonyms: 2010103A03Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200288 representing BC028529
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAGTCGACTGCAGGAGCTCCGCAAGGAGGAGGAAACCCTGCTGCGTCTAAAGGCGGCTCTACACG
  ACCAACTGAACCGCCTCAAGGTTGAAGAATTAGCCCTTCAATCCATGATAAATTCTCGAGGAAGGACCGA
  GACACTGTCTTCTCAGCCTGCACCTGAACAGTTATGTGATATGTCCCTACATGTAGACAACGAAGTGACA
  ATAAATCAGACTACACTGAAGCTGAGCACAAGGAGCCCTATGGAAGAAGAAGAGGAGGAAGAGGAGGAGG
  AAGAATCTGATTCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200288 representing BC028529
  Red=Cloning site Green=Tags(s)
  
  MLSRLQELRKEEETLLRLKAALHDQLNRLKVEELALQSMINSRGRTETLSSQPAPEQLCDMSLHVDNEVT
  INQTTLKLSTRSPMEEEEEEEEEEESDS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC028529
• ORF Size: 296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028529, AAH28529
• RefSeq Size: 762 bp
• RefSeq ORF: 296 bp
• Locus ID: 330959
• Cytogenetics: 9 C
• Gene Summary: Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box (By similarity).[UniProtKB/Swiss-Prot Function]