Mical3 (NM_001270475) Mouse Untagged Clone

CAT#: MC229691

Mical3 (untagged) - Mouse microtubule associated monooxygenase, calponin and LIM domain containing 3 (Mical3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mical3
• Synonyms: C130040D16Rik; Mical-3; mKIAA1364
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229691 representing NM_001270475
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGAGGAAGCAGGAGACAACGAACCAAGCTCACGTCCTCTTTGACCGCTTTGTCCAGGCCACCA
  CCTGCAAGGGGACCCTCAGGGCCTTCCAAGAGCTCTGTGACCACCTGGAGCTGAAGCCCAAGGACTACCG
  GTCCTTCTACCACAAGCTCAAGTCCAAGCTCAACTACTGGAAGGCCAAAGCCCTCTGGGCCAAATTGGAC
  AAGCGGGGCAGTCACAAAGACTACAAAAAGGGCAAAGCCTGCACCAACACAAAGTGTCTCATCATTGGAG
  CTGGCCCCTGTGGGCTGCGCACAGCCATCGACTTGTCCTTGTTGGGAGCCAAGGTGGTTGTTATTGAAAA
  GCGAGATGCCTTCTCCCGCAACAATGTCTTACATCTCTGGCCCTTCACCATACACGATCTCCGAGGCCTG
  GGTGCCAAGAAATTCTACGGCAAGTTCTGTGCCGGAGCCATCGACCATATCAGTATCCGTCAGCTCCAGC
  TAATCCTCTTGAAGGTAGCCTTGATCTTGGGCATTGAGATCCATGTCAATGTGGAATTCCAAGGACTTGT
  TCAGCCTCCCGAGGACCAAGAGAATGAACGGATAGGATGGCGAGCATTGGTGCACCCCAAAACTCATCCT
  GTATCAGAATATGAATTTGAAGTTATCATCGGTGGAGATGGGCGTAGGAACACCTTGGAAGGATTTCGTC
  GAAAAGAATTTCGTGGCAAGCTAGCCATTGCCATTACAGCAAATTTTATCAACCGCAACACAACCGCAGA
  GGCCAAAGTGGAGGAGATTAGTGGCGTGGCTTTTATATTCAACCAAAAATTTTTCCAGGAGCTGAGAGAA
  GCCACAGGTATTGACTTAGAGAACATCGTCTACTACAAAGATGACACACACTACTTTGTCATGACGGCCA
  AAAAGCAGAGTTTGCTGGACAAGGGGGTGATCCTGCATGACTACACAGACACGGAGCTGTTGCTTTCCCG
  GGAGAACGTGGACCAGGAGGCCCTGCTGAACTATGCCCGGGAGGCCGCAGACTTCTCTACCCAGCAGCAG
  CTGCCTTCTCTGGATTTCGCCATCAATCACTACGGGCAGCCAGACGTGGCCATGTTTGACTTTACCTGCA
  TGTACGCCTCTGAAAATGCCGCCTTGGTCCGGGAACAGAACGGACACCAGTTACTAGTAGCTTTGGTTGG
  AGACAGCCTCCTGGAGCCTTTCTGGCCAATGGGGACAGGAATAGCCCGGGGCTTTCTGGCTGCTATGGAC
  TCTGCCTGGATGGTACGAAGCTGGTCTCTGGGCACCAGCCCCTTGGAAGTCCTGGCAGAAAGGGAAAGCA
  TTTATAGGTTGCTGCCTCAGACCACCCCTGAGAACGTGAGCAAAAACTTCAGCCAATATAGCATCGACCC
  TGTCACTAGGTATCCCAATATCAACATCAACTTCCTCAGGCCAAGCCAGGTGCGCCATTTATACGATAGC
  GGAGAAACAAAAGATATCCACCTGGAAATGGAGAACATGGTGAATCCCCGAACCACTCCCAAGCTGACTC
  GCAACGAGTCTGTAGCTCGTTCAAGCAAACTGCTGGGGTGGTGCCAAAGGCAGACAGAAGGCTATTCAGG
  AGTCAATGTGACAGATCTCACTATGTCTTGGAAAAGTGGCCTGGCCCTGTGTGCGATCATCCACAGATAC
  CGCCCCGATCTAATAGACTTTGATTCTTTGGATGAGCAAAACGTGGAGAAGAATAATCAGCTGGCCTTTG
  ACATCGCTGAGAAGGAACTGGGTATCTCTCCCATCATGACGGGCAAGGAGATGGCTTCGGTCGGGGAGCC
  AGACAAGCTGTCCATGGTGATGTACCTCACGCAGTTCTACGAGATGTTCAAGGACTCGCTCTCCTCCAGC
  GACACCCTCGACCTGAATGCAGAGGAGAAAGCCGTCCTGATAGCCAGCACCAAATCCCCCATCTCCTTCC
  TGAGCAAACTCGGACAGACCATCTCTCGGAAGCGTTCGCCCAAGGATAAAAAAGAAAAGGACTCAGATGG
  GGCTGGAAAGAGGAGAAAAACCAGTCAGTCAGAGGAGGAGGAGCCTCCCCGGAGCTACAAAGGAGAAAGA
  CCGACCCTGGTGAGCACCCTGACGGACAGACGGATGGATGCTGCCGTTGGGAACCAGAACAAAGTGAAGT
  ACATGGCGACCCAGCTGCTGGCCAAGTTTGAAGAGAACGCACCTGCTCAGTCCACTGGTGTGAGGAGACA
  GGGCTCCATAAAGAAAGAGTTCCCGCAGAACTTGGGGGGCAGCGACACGTGCTATTTCTGCCAAAAGCGG
  GTCTACGTGATGGAGAGGCTGAGCGCCGAGGGCAAGTTCTTCCATCGAAGCTGCTTCAAGTGTGAGTACT
  GTGCCACCACGCTGCGCCTATCAGCCTACGCCTACGACATCGAGGATGGCAAATTCTACTGTAAGCCGCA
  CTACTGTTACCGACTATCTGGCTACGCGCAAAGGAAGAGGCCAGCGGTGGCTCCTCTGTCTGGAAAGGAA
  GTCAAAGGAGCCCTGCAGGATGGCCCCACTGCAGATGCAAATGGACTGGCCAGCGTTGCCGCCAGCTCAG
  CCGAGAGAAGTCCAGGTACCAGCATGAACGGCCTGGAGGAGCCCAGCATTGCCAAAAGGCTGAGGGGCAC
  CCCCGAGAGGATCGAGCTGGAGAACTACCGCCGATCTGTGAGGCAGGTGGAGGAGCTGGAGGAGGTACCC
  GAGGAGACACAGGCCGAGCATAACCTGAGCAGCGTCTTAGACAAAGGCACCGAAGAGGATGTGGCCAGCA
  GCAGTTCTGAGTCGGAGATGGAGGAGGAGGAAGAGGAAGATGACGAAGATGATCATCTCCCCACCTCTGA
  CCTGGGCGGGGTTCCCTGGAAGGAGGCAGTCAGGATCCATGCCCTTCTGAAGGGAAGGAGTGAAGAGGAG
  CTGGAAGCCTCCAAGAACTTCGAGCCCGAGGAAGAAGAAGAGGAGGAGGAATACGAAGAAGAGGATGAAG
  AGTATGAGGAGGAGGAAGAGGAGGAATCCAGTGAAGCCGGGAACAAGAGGCTGCAGCAGATCATAACCGC
  AGCGGATCCCTTGGCGATCCAGGCTGATGTGCACTGGACACACATCCGTGAGAGAGAGGCAGAGGAGAGG
  ATGCTCCCAACCTCTGAGTCCTCCACTTCTAGAGCTCCTCTAGATGAGGATGACCTAGAAGAGGACGCGG
  ACTCTGAGCCAGCAGAGACTGAAGGGGAAGCCGCGGAGGATGGGGACCCTGGAGACACCGGTGCTGAACT
  GGATGATCAGCACTGGTCTGACGACATCCCTTCTGATGCTGAAGCGGAGCATCGCTTACAGAGCCAGGCC
  AAGGTGAAAGCAGAGCTGGAGCTGAGAGTATCGGAAAACGAGGAAGAGAAGCCATCTGATGCGCCAAAGC
  AAGAGGAGAGAGGTACTTCCCAAGTCTCCAGCCCTAGCCAGCCACCTGAGAAACAAGTTGGTGTGTTCTC
  CCCAGCTCGCAGCCCTGGGACAGAGGAGGCCAAATCCCCACTTGCCACCAAGGTAAAGTCCCCCGAGGAG
  CCACTTTTCCCCACGCCTCTGCTCCTCAGAGAAAAGCCCAAAGCTGAAGTCCCTGAAGAACAGAAAGCTG
  TACTCTCGCCCATCCGGTCACAGCCTGTGGCCCTGCCAGAAGCCAGATCACCTACCTCACCCACCAGCTT
  ACAGCCAGAGTCTCTGTTGGCCCCTCCCACTCCTCCCACACCTCCCCCCACCCAACTCCCCATCTGTTCC
  CAACCTCAGCCCTCCTCGGATGCCTCCATCCCATCGCCCACCAAGTCCCCCATACGCTTCCAGCCAGTTC
  CAGCCAAAACATCCACACCCCTCACCCCACTCCCCGTGAAGAGCCAAGGAGACCCCAAAGACAGACTGAG
  TGGCCCCCTGGCTGTGGAGGAGGTCTTAAAGCGGAGTGATCTGGTGGAAGAATTCTGGATGAAGAGTGCT
  GAGATCCGCCGCAGCCTGGGCCTTACTCCCGTGGACCGGAGCAAGGGGTCAGAGCCCAGCCTCCCCTCCC
  CTGCCTCGAAGCCAATATCCCTAAAGTCCTACTCAGTTGACAAGTCACCTCAGGATGAGGGTCTCTGCCT
  TCTGAAACCTCCATCGGTTCCTAAAAGGTTGGGCCTACCAAAGTCAGCCGGTGACCAGCCCCCACTGCTG
  ACCCCAAAGTCGCCCTCCGACAAGGAGCTGAGGAGCAGCCAGGAGGAGCGAAGGGACCTGTCCAGCAGCT
  CAGGCCTGGGCCTCCATGATAGCTCCTCCAACATGAAGACCTTGGGCAGCCAGAGCTTCAACACCTCGGA
  CTCCACCATGCTCACCCCACCTTCCAGCCCACCCCCACCCCCACCCCCCAACGAGGAGCCTGCAACCCTT
  CGAAGGAAACCCCATCAGACTTTTGAGCGCAGGGAGGCCTCAATTATCCCCCCTCCCACCCCTGCCTCAT
  TCATGCGGCCCCCACGGGAGCCGGCCCAGCCCCCCAGGGAGGAGGTTCGGAAGTCCTTCGTGGAGAGTGT
  GGATGAGATCCCCTTTGCCGACGATGTGGAGGACACTTACGATGATAAGACGGAGGACTCCAGTCTGCAG
  GAGAAGTTCTTCACACCCCCTTCCTGCTGGTCCCGCTCGGAGAAGCTCCAGGCCAAGGAGAACGGGCGAC
  TCCCACCCCTGGAGCAGGACGTGCCACCACAGAAAAGGGGGCTGCCCCTGGTCTCCGCGGAAGCCAAGGA
  GCTGGCCGAGGAGCGCATGCGCGCCCGGGAGAAGTCTGTGAAGAGCCAGGCACTGCGAGATGCCATGGCC
  AAACAGCTGAGCCGGATGCAGGCCATGGAGATGGTGTCCTCCAGGTCCCACACGGCACAATCGCAGGGCA
  AGGAACTAGGATCCGAGTCCACCAGACACCCAAGCCTCCGAGGCACCCAGGAGCCCACCCTGAAGCATGA
  AGCCACCAGCGAAGAGATCCTCTCCCCTCCGTCAGACTCAGGAGGCCCCGATGGCTCCGTCACCTCATCT
  GAGGGCTCCAGTGGAAAGAGCAAAAAGAGGTCGTCGCTCTTCTCCCCCCGCAGGAATAAGAAAGAGAAGA
  AAACCAAAGGGGAAGCCCGGCCCCCCGAGAAACCCAGCCCAGGCCTCCCAGAAGATGTGGTAGCCAAGCC
  CAAGTCCCTGTGGAAATCTGTCTTCTCTGGGTACAAGAAGGACAAGAAGAAAAAGAGCGACGAGAAATCG
  TGTTCCAGCACGCCGTCCAGTGGCGCCACGGTGGACTCCGGCCAGCGCAGGGCGTCTCCAATGGTGAGAG
  CAGAGCTGCAGCTCCGCCGCCAGCTGAGTTTCTCAGAGGACTCAGACCTGTCCAGTGATGACATCCTTGA
  GAGGTCTTCCCAGAAGTCCAAGCGAGAGCCCAGAACCTACACAGAGGAGGAGCTGAGTGCCAAGCTGACG
  AGGCGTGTGCAGAAGGCGGCTCGGAGACAGGCTAAGCAGGAGGAACTGAAGCGGCTGCACCGAGCCCAGA
  TCATCCAGCGGCAACTGGAGCAAGTGGAAGAGAAACAGAGGCAGCTGGAGGAGAGGGGCGTTGCAGTAGA
  GAAGGCTCTTCGAGGAGAAGCAGGCATGGGCAAAAAGGATGACCCCAAGCTGATGCAGGAGTGGTTCAAG
  CTGGTGCAGGAGAAGAACGCAATGGTGCGCTACGAGTCGGAGCTGATGATTTTTGCCCGGGAGCTGGAGC
  TGGAAGACCGGCAGAGCCGACTACAGCAGGAGCTGAGGGAGCGGATGGCCGTGGAAGATCACCTGAAGAC
  CGAGGGGGAGCTTTCAGAGGAGAAGAAGATCCTGAACGAGATGCTGGAGGTGGTGGAGCAGAGAGACTCG
  CTGGTGGCCCTGCTGGAGGAGCAGCGACTGCGGGAGAAAGAGGAGGACAAGGACCTGGAGGCCGCCATGC
  TATGCAAAGGCTTCAGCCTGGACTGGTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270475
• Insert Size: 5982 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270475.1, NP_001257404.1
• RefSeq Size: 9448 bp
• RefSeq ORF: 5982 bp
• Locus ID: 194401
• UniProt ID: Q8CJ19
• Cytogenetics: 6 F1
• Gene Summary: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Seems to act as Rab effector protein and play a role in vesicle trafficking. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process and implicates RAB8A associated with exocytotic vesicles. Required for cytokinesis. Contributes to stabilization and/or maturation of the intercellular bridge independently of its monooxygenase activity. Promotes recruitment of Rab8 and ERC1 to the intercellular bridge, and together these proteins are proposed to function in timely abscission.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).