Rapgef6 (NM_001252496) Mouse Untagged Clone

CAT#: MC229615

Rapgef6 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 6 (Rapgef6), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rapgef6
• Synonyms: A530068K01; AI844632; BB085664; C030018K18Rik; mKIAA4052; PDZ-GEF2; Pdzgef2; RA-GEF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229615 representing NM_001252496
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCGCCCGTGGACCCCGGCGCTCGGCAGGCACTGAGGAAGAAGCCGCCCGAGCGGACTCCTGAGG
  ACTTAAATATTATTTATTCTTATCTGCATGGGATGGAAATACTATCAAACCTCAGGGAGCATCAGCTTAG
  GTTAATGTCTACAAGAGCACGCTATGAGAGATACAGTGGCAATCAAATGCTTTTTTGTTCAGAAACTATT
  GCCAGATGTTGGTATATCCTGCTTTCTGGATCTGTACTTGTAAAAGATTCTATGGTCCTTCCTCCTTGCA
  GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTGTTAGAACCTTCAGAAATGATTGT
  GGTAGAGAATTCCAAAGATAATGAAGACAGTATTTTACAAAGAGAAATTCCTGCCAGACAGTCTCGACGA
  AGGTTTCGGAAAATTAACTATAAAGGAGAACGCCAAACCATAATAGATGGAGTGGATATTAATAACTACC
  TTTCGCTTCCAGCTGATCTTACCAAGATGCACCTCACAGACAACCCTCATCCACAGGTGACGCATGTGTC
  TTCTAGTCAGTCTGGCTGTAGCATTGCCAGTGACTCTGGGAGCAGCAGTTTATCCGATATCTATCAGGCT
  ACAGAGAGTGAAGTAGGAGATGTAGATTTAACACGCCTTCCAGAAGGACCTGTTGATTCTGAGGATGAAG
  AGGAGGAGGAAGAAGAGATTGATAGAACAGATCCACTGCAGGGTCGAGATCTTGTTCGGGAGTGTCTTGA
  AAAGGAACCCGCAGACAAAACTGATGATGATGTTGAACAGTTACTTGAGTTCATGCACCAGCTGCCTGCC
  TTTGCGAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCCGTCATGGTTTTTGAAGTAGTAGAGCAGG
  CTGGGGCTGTTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTCATTTTAAATGGCACTGTAGA
  AATTAGTCATCCAGATGGGAAAATAGAAAATCTCTTCATGGGAAATAGTTTTGGGATTGTCCCCACGCTG
  GATAAGCAGCACATGCATGGAGCTGTCAGGACCAAAGTCGATGATTGTCAGTTTGTCTGCATAGCTCAAC
  AAGACTATTGGAGAATTTTAAATCATGTGGAAAAAAATACTCACAAAGTTGAGGAAGAGGGAGAGATTGT
  CATGGTACATGAGCACCGTGAGCTAGACCGGAGCGGAACCAGGAAAGGACACATTGTAATCAAGGCAACA
  CCTGAGCGTCTCATCATGCATTTGATAGAAGAACATTCTATTGTGGACCCAACATACATAGAAGATTTCC
  TCTTAACTTACAGGACATTTCTTGAAACTCCTTTGGATGTTGGGATCAAGCTTTTGGAATGGTTTAAGAT
  TGACAACCTAAGGGATAAGGTGACACGGATTGTACTCTTATGGGTAAATAATCATTTTAATGATTTTGAA
  GGTGATCCTGCTATGACTCAATTTCTAGAAGAATTTGAAAGAAATTTGGAAGATACAAAAATGAATGGTC
  ACCTCAGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGGCAAGTTGTACTGCAGAAGGCGTC
  CCGTGAGTCCCCGCTGCACTTCTGCCTTACTGGGGGCAGTGAGAAGGGATTTGGAGTCTTTGTTGAAGAA
  GTAGAATCTGGTAGCAAAGCTGCTGACGCAGGACTGAAAAGGGGTGATCAGGTAATGGAAGTCAATGGAC
  AAAACTTTGAGAATATTACACTTGCTAAAGCCCTTGAAATTTTGAGAAATAATACTCATCTTGCACTTAC
  TGTGAAGACCAACATTTTTGTGTTCAAAGAATTGCTCAGTAGGACTGAACAAGAGAAATCTGGTGTTCCT
  CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCAATTCAAGATGTCCCAGGGGATATGGAGC
  AGGCACCACAGGAGAAAGGAAATAAGAAAATTAAAGCAAATACCGTTTCAGGTGGAAGAAACAAAATCAG
  GAAAATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAACTATTTAGTGACGGAGGCCTGAGTCAA
  TCACAAGATGACAGCATTGTGGGAACCAGGCACTGTAGGCACAGCCTGGCTATAATGCCAATTCCCGGAA
  CACTTTCATCCAGCAGCCCAGATCTCTTACAGCCTACCACTAGCATGTTGGACTTTTCCAATCCTTCAGC
  TGTTGGTTTTTACTATATTCCTGATCAAGTTATAAGAGTTTTCAAAGCAGATCAACAAAGTTGCTACATT
  ATCATCAGTAAGGATACCACAGCTAAAGAAGTGGTCTGCCAAGCTGTACAGGAATTCGGTTTGACTGGTG
  CATCTGACACATACTCCCTCTGTGAAGTGTCTGTTACTCCTGAGGGTGTTATAAAGCAGAGAAGACTTCC
  GGACCAGTTCTCCAAATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACCTAAAAAACAACATGGAA
  ACAGAAACATTGTGTTCTGATGAAGATGCTCAGGAACTACTTAAGGAAAGCCAGCTCTCCATGCTGCAGC
  TCAGTACCATCGAGGTCGCCACCCAGCTCTCCATGAGAGACTTTGATTTATTTCGTAACATTGAGCCTAC
  TGAATACATTGATGATCTTTTTAAATTAGATTCCAAAACAGGAAATACTCACTTGAAACAATTTGAGGAT
  ATTGTAAACCAAGAGACATTCTGGGTTGCCTCAGAGATTTTAAGTGAGTCAAATCAGCTGAAAAGAATGA
  AGATTATTAAACATTTTATCAAAATTGCTCTTCATTGCCGAGAATGCAAGAATTTCAATTCCATGTTTGC
  AATAATAAGTGGCTTGAACCTGGCACCGGTAGCACGACTCAGAGGCACTTGGGAAAAGTTGCCAAGCAAA
  TATGAGAAACATCTTCAAGATCTACAAGACCTTTTTGATCCATCTAGAAATATGGCAAAATACAGAAATA
  TTCTTAGTAGTCAAAGCATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATT
  TCTCCATGAAGGAAATGACTCCAAAGTAGATGGCTTAGTAAACTTCGAGAAGCTAAGAATGATTGCCAAG
  GAAATCCGTCATATTATTCGAATGACTTCTGCCAACATGGACCCTGCCATGATGTTCCGACAGAGGAAGA
  AGAGGTGGCGGAGTCTGGGGTCATTGAGTCAAGGCAGCACAAATTCTAACATGCTAGATGTCCAGGGAGG
  TGCTCACAAAAAAAGAGCACGCCGCAGCTCACTGCTGAATGCCAAGAAACTGTATGAAGATGCCCAAATG
  GCAAGGAAAGTGAAACAGTATCTTTCTAGTCTGGACATAGACACAGATGAGGAGAAGTTCCAGATGATGT
  CACTGCAGTGGGAACCTGCATATGGTACCTTGACAAAGAACTTAACTGAAAAAAGGTCAGCCAAATCATC
  TGAGATGTCTCCTGTACCATTGAGGTCAGTTGGCCAAACAGCTAAAGTCCACTTGCATCAGCCACACAGA
  GTGAGCCAGGTGCTTCAGGTGCCAGCAGTTAACTTGCACCCCATCCGGAAGAAAGGACAAGCCAAAGACC
  ATGTGCTGAGTACAAGTTTACCTCAGAAAGGTTTAGGACCAACTGAGGAAGTGAGTGTTAAGAAACACAC
  AGAAGACACTATTTCTGTAGCGTCCTCTCTGCACTCTAGCCCTCCTGCATCTCCTCAGAACTCCCCACGC
  AAAGGTTATACACTCACACCATCAAGTAAATGTGACAACTTGTCTGACTCCAGCCACAGTGAAATCTCCT
  CTCGGTCCAGCATTGTGAGCAATGGTTCTGTTGACTCCATGTCTGCAGCTGGGCAGGATGAACGCTGTTC
  CTCCCACAGTCTGGCAGTTCCTGAGCCCACAGGGGCATTGGAAAAGACAGATCACCCTTCCGGGATCTCA
  GACCACAGTCAGCTTGCTCACGGGTGGATGCTGTCAAAGCCGTGTCTTATCAAGGGTGTAGCTGTCTCAT
  CTTCTCTGAGCAGCGAAGAGATGTCTCATGAGCATGTTGTGCTGGAAGCAGCTGACAGTGGGCGGGGCAG
  TTGGACTTCCTGTTCCAGCAGCTCCCATGACAACTTCCAAAGCCTTCAAAATCAAAAAAGCTGGGACTTC
  CTGAATTCTTACAGACACATGCATTTAGATGACCCTATTGCAGAAGTTGAGCCCACTGACTGTGAGCCCT
  GTGCCTGTCCTAAAGGCTGCTCAAGAACTTGTGGCCAGTGCAAAGGCAGCCTAGAGACGAACCAGCTGAG
  GCAGAGTTGGGCCTCCTCCAGCTCACTCTCGGACACATGTGAACCAAACTACGGGACAGTTAAGCGGAGG
  GTGTTGGAAAGTGCACCAGCAGAGGCACCTGATGGCTTGGAGCCCAGGGATACCACTGACCCTGTTTATA
  AAACTGTCACTTCCAGTACAGACAAGGGCTTGATTGAAAACGAGCAGGTGTCAGCAGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252496
• Insert Size: 4542 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252496.1, NP_001239425.1
• RefSeq Size: 7945 bp
• RefSeq ORF: 4542 bp
• Locus ID: 192786
• Cytogenetics: 11 B1.3