Fhod3 (NM_001289654) Mouse Untagged Clone

CAT#: MC229588

Fhod3 (untagged) - Mouse formin homology 2 domain containing 3 (Fhod3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fhod3
• Synonyms: A930009H06Rik; FHOS2; mKIAA1695
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229588 representing NM_001289654
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGCTGGCTTGTCGCGTGCAGTTCTTGGACGACACCGATCCTTTCAACAGCACCAACTTCCCAG
  AGCCAAGCCGGCCACCGCTGTTCACATTCCGCGAGGACCTGGCTCTGGGCACCCAGTTGGCCGGGGTCCA
  TCGGCTGCTGCGGGCGCCGCACAAGCTCGATGACTGTACCCTGCAGCTGTCCCACAATGGCGCCTACCTG
  GATCTGGAGGCCACGCTGGCGGAACAGCGGGATGAGCTAGAAGGCTTCCAGGATGACACAGGGCGGGGCA
  AGAAGAACAGCATTATTCTGAGGACACAGCTGTCTGTGAGGGTCCATGCCTGCATTGAAAAGCTGTACAA
  CTCCAGTGGCCGAGATCTAAGAAGGGCCCTTTTCTCACTGAAGCAGATATTTCAGGATGACAAGGATTTG
  GTGCATGAATTTGTCATAGCTGAAGGTCTGACCTGTTTGATCAAAGTGGGAGCCGAGGCGGATCAGAACT
  ATCAGAACTACATCCTGAGGGCTCTGGGTCAGATTATGTTGTATGTGGATGGGATGAATGGCGTCATCAA
  CCACAGCGAGACCATCCAGTGGCTTTACACGCTCGTTGGGTCGAAGTTCCGCCTGGTAGTGAAGACAGCC
  CTGAAGCTTCTTCTGGTCTTTGTGGAGTATTCTGAGTCCAATGCACCACTTCTCATTCAGGCCGTTTCTG
  CTGTCGACACCAAAAGAGGTGTCAAACCCTGGTCCAACATCATGGAAATCTTGGAGGAAAAAGATGGAGT
  TGACACAGAGCTATTGGTTTATGCAATGACTCTAGTGAACAAGACACTGGCAGGACTGCCAGATCAAGAC
  ACCTTCTATGACGTAGTAGACTGCCTGGAAGAGCTGGGCATCGCCGCTGTGTCCCAAAGGCACTTGAACA
  AGAAAGGGACAGACCTGGACTTGCTGGAGCAGTTTAATATCTATGAGGTGGCACTCAGGCATGAAGATGG
  TGATGAGACAGCTGAGCCACCCCCCAGTGGGCACCGGGACCGGAGGAGGGCCAGCATGTGTTCTGGTGGC
  ACTGTGGGTGAGCAACAGGGCCTTGACCGCAGAAGGAGCCGTAGACACTCAATCCAGAATATCAAGAGCC
  CACTGTCAGCTCCCACCAGCCCCTGCTCCCCGTCTGTTCCTGCCTTCAAACCCAGCCAAGTTCGAGACCT
  CTGTGAAAAATACAGCAATTTCAGCAGCAACTCTTTCCAATCCTCCAGACCCTCTCCTGGACCTAGTGGG
  TCCCCATCTTATGCATCATCCTTCTCATCCCCACAGGATACCAGGTCATCGCCAAGTGGCCTTCTCACAT
  CCTCGTTCAGGCAGCACCAAGAGTCACTGGCAGCAGAGAGAGAGCGGAGGCGGCAAGAGAGAGAGGAAAG
  ACTGCAGAGGATAGAACGGGAAGAAAGGAACAAATTCAACCGGGAATATCTAGACAAAAGGGAGGAGCAA
  AGGCAAGCAAGAGGAGAAAGATACAAATACTTGGAGCAGTTGGCAGCCGAGACGCAGGAGAAGGAGCCTA
  GAAGCCAGAGCGTGAGCCGGGGTAGAGCTGACCTCTCCTTGGACCTGAGCTTGCCTGCAGCCCCTGCTCC
  ACCTTCCCCTTCAAGCCAGAGTCCCTCTGCTGACTCACAAGAGGCTCTCCCTGTGCCTTCATCCCCACCC
  ACTCTGCAGTGCCCCCAAGTAAGTGGTAAGGACCACGAGCCTGAACTGGAGGCAGAAGCTGGGCAAGGTG
  CTGATGAAGCCAGCCAGGATATAGCATCTGCCCACAGGGGAGCAGAGAGCCAAGAAGAGCCGGTGCTGGA
  GCTAGAGCCAGAGGAAAGAGCTTCACTGAGTGAAAAAGAGAGGCAAAACGAAGAGGTGAATGAGAGGGAC
  AACTGTTCTGCTTCCAGCATCTCGTCCTCCAGCAGCACCTTGGAGAGGGAAGAGAAGGAAGACAAACTGT
  CTGAAGACAGGGCGACCGGTTTGTGGTCCACCAGCCTTCAGGATGTGGGTGTGAATGGACAGTGTGGTGA
  CATCCTTACCAGCAAAAGGTTCATGCTGGACATGCTGTATGCCCATAACAGAAAATCCACAGAAGATGAG
  GAAAAAGATGACGGCGAGCCTGGGAGGTCTGCGCAGGAGGTGGAGGCAGTGGCCAGCCTCGCCACCAGGA
  TATCCACTCTGCAGGCCAACTCTCAGGCCCCAGAGGAGAGCATCAAGAGGGTGGACATTGGCTGTCTAGA
  CAACCGGGGCAGTGTGAAGGCATTTGCTGAGAAATTCAACAGTGGAGAAGTAGGGAGGGGTGCCATCTCT
  CCTGATGTGGAGTCTCAGGACAAGGTCCCGGATACACCCCCAGCACAGCTGAAGACTGAGTCTGATTACA
  TCTGGGACCAGCTCATGGCCAATCCGAGGGAGCTCAGAATCCAAGACATGGATTTCACAGACCTAGGGGA
  GGAGGACGATATCGATGTTTTGGATGTGGATCTTGGCCACCGGGAAGCCCCAGGGCCACCCCCTCCACCC
  CCTCCCACCTTCCTGGGTTTGCCACCCCCACCCCCTCCACCCCTGTTGGACAGCGTGCCTCCCCCACCAG
  TCCCCGGTAATTTATTGGCTTCTCCTGTGTTCAACACTCCTCAGGGCTTAGGGTGGTCCCAGGTACCCAG
  GGGTCAGCCAGCGTTCACCAAGAAAAAGAAGACGATCCGGCTGTTCTGGAACGAAGTCCGGCCCTTTGAG
  TGGCCAAGTAAGAACAATCGCCGGTGCAGGGAATTCCTGTGGTCAAAACTGGAACCCATTAAGGTGGACA
  CTTCCAGACTCGAGCACCTGTTTGAGTCCAAGTCTAAGGAGCTGTCTGTCACCAAGAAAACGGCTGCAGA
  TGGTAAACGGCAGGAGATCATTGTTCTGGATTCCAAGAGGAGCAATGCCATCAACATCGGCCTGACGGTG
  CTGCCCCCTCCAAGGACAATTAAGATAGCCATTTTGAACTTTGATGAGTATGCCTTAAACAAAGAAGGGA
  TCGAGAAAATTCTGACCATGATTCCAACTGAAGAGGAGAAGCAGAAAATCCAGGAGGCTCAGCTGGCTAA
  CCCGGAAGTGCCGTTGGGCAGTGCAGAGCAGTTCCTTCTCACACTCTCTTCCATCAGTGAGCTCTCGGCT
  CGGCTCCACCTGTGGGCATTCAAAATGGATTATGAAACCACAGAAAAGGAAGTGGCCGAACCACTTTTGG
  ACCTGAAGGAAGGAATAGACCAGTTGGAGAACAATAAGACTCTGGGCTTCATCCTGTCTACTCTCCTTGC
  CATTGGGAACTTTCTAAATGGAACTAATGCCAAAGCATTTGAGTTAAGCTACCTCGAGAAGGTTCCAGAA
  GTCAAAGATACAGTGCACAAGCAGTCTCTTCTCCACCACGTGTGCACCATGGTAGTGGAAAACTTTCCGG
  ACAGCTCGGATCTGTACTCAGAGATTGGGGCCATCACTAGGTCAGCCAAGGTTGACTTTGATCAACTTCA
  GGATAATTTATGTCAGATGGAGAGACGCTGCAAAGCTTCATGGGACCACCTCAAGGCGATTGCAAAACAC
  GAGATGAAGCCTGTTTTGAAACAGCGGATGTCAGAGTTCCTAAAAGACTGTGCAGAGCGAATTATAATTT
  TAAAGATCGTCCATCGGAGGATAATTAACAGATTTCACTCATTTTTACTGTTTATGGGCCATCCCCCTTA
  TGCAATTCGAGAGGTGAACATCAACAAGTTCTGCAGGATCATCAGTGAGTTTGCCCTGGAGTATCGCACG
  ACCAGGGAAAGGGTTCTACAGCAGAAACAGAAGCGGGCCAACCACAGAGAGAGAAACAAGACCAGAGGGA
  AGATGATCACTGACTCTGGCAAGTTCTCTGGCAGTTCTCCAGCAGCACCGAGCCAGCCACAGGGTCTGAG
  CTATGCTGAGGATGCAGCCGAACATGAGAACATGAAGGCCGTGTTGAAAACCTCCTCCCCTGCGCTGGAG
  GACGCCACCCCAGTGCTTGGTGTCCGCACTCGCAGCCGGGCAAGCCGAGGATCCACTAGTTCATGGACTA
  TGGGAACTGAGGAGTCACCTAGTGTCACTGATGATGCGGCTGATGAGATCATGGACCGCATTGTCAAATC
  AGCCACCCAAGTGCCCAGTCAGAGAGTGGTGCCACGGGAGAGAAAGCGTTCCAGGGCCAACCGCAAATCT
  TTGCGAAGGACCTTGAAGAGTGGCCTGACTCCAGAAGAAGCCAGAGCCCTGGGCCTGGTTGGCACATCTG
  AACTGCAACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289654
• Insert Size: 4284 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289654.1, NP_001276583.1
• RefSeq Size: 5798 bp
• RefSeq ORF: 4284 bp
• Locus ID: 225288
• UniProt ID: Q76LL6
• Cytogenetics: 18 A2
• Gene Summary: May play a role in actin filament polymerization in cardiomyocytes (By similarity). Actin-organizing protein that may cause stress fiber formation together with cell elongation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1.