Flii (NM_001302207) Mouse Untagged Clone

CAT#: MC229534

Flii (untagged) - Mouse flightless I homolog (Drosophila) (Flii), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Flii
• Synonyms: 3632430F08Rik; Fl; Fliih
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229534 representing NM_001302207
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCACCGGGGTGCTGCCGTTCGTGCGCGGCGTGGACCTCAGCGGCAACGACTTCAAGGGTGGCT
  ACTTCCCTGAGAATGTTAAGGCTATGACCAGCCTGCGATGGCTAAAGCTGAATCGCACAGGCCTCTGCTA
  CCTGCCTGAAGAACTTGCGGCTCTGCAGAAGCTGGAGCACCTGTCTGTGAGCCACAACCATCTGACCACG
  CTTCACGGCGAACTGTCTAGCCTGCCTTCGCTTCGGGCCATTGTAGCTCGGGCCAACAGCCTGAAGAATT
  CCGGAGTCCCTGATGACATCTTCAAGCTGGATGATCTCTCTGTTCTGGACTTGAGCCATAACCAGTTGAC
  AGAATGCCCACGGGAGCTGGAGAATGCCAAGAACATGCTGGTGCTCAACCTCAGCCACAACGGCATCGAC
  TCCATCCCCAACCAGCTGTTCATCAACCTTACGGACTTGCTGTACCTGGACCTCAGTGAAAACCGCCTAG
  AGAGCCTTCCCCCTCAAATGCGCCGCCTCGTGCACCTTCAGACACTGGTGCTGAATGGAAACCCGCTGCT
  ACATGCACAGCTCCGGCAGCTCCCAGCCATGATGGCCCTACAGACCCTGCATCTGAGGAACACTCAACGC
  ACCCAGAGCAACCTCCCCACCAGCCTGGAGGGCCTGAGCAACCTTTCAGATGTGGACCTGTCGTGCAATG
  ACTTGACAAGGGTGCCGGAGTGCCTGTACACCCTCCCCAGCCTGCGCCGCCTCAACCTCAGTAGCAACCA
  GATTGCAGAGCTGTCCCTGTGCATCGACCAGTGGGTGCACCTGGAGACCTTGAATCTGTCCCGGAACCAG
  CTCACCTCCCTGCCTTCAGCCATCTGCAAGCTCACCAAGCTGAAGAAGCTGTACCTCAACTCCAACAAGC
  TGGACTTTGATGGGCTGCCTTCTGGCATCGGCAAGCTCACCAGCCTGGAGGAATTCATGGCTGCCAACAA
  CAACCTAGAACTGATTCCTGAAAGTCTCTGCAGGTGCCCAAAACTGAAGAAACTCGTCCTGAATAAGAAC
  CGCCTGGTGACCCTTCCTGAGGCCATCCACTTCCTGACAGAGATCCAGGTCCTGGATGTTCGAGAAAACC
  CCAGCTTGGTCATGCCGCCCAAGCCTGCTGACCGCACCGCCGAGTGGTACAACATTGACTTCTCGCTACA
  GAACCAGCTGAGGCTGGCGGGCGCCTCCCCGGCCACAGTGGCTGCTGCTGCAGCTGGGAGCGGGTCCAAG
  GACCCCTTGGCTCGCAAGATGCGGCTACGGAGGCGCAAGGACTCAGCACAGGATGTCCAGGCCAAGCAAG
  TGCTAAAGGGCATGTCAGATGTTGCCCAGGAGAAAAACAAAAACCAAGAGGAAAGCATAGATGCCCGGGC
  ACCAGGGGGAAAGGTACGACGCTGGGACCAGGGCCTGGAGAAGCCACGCCTTGACTACTCGGAGTTCTTT
  ACGGAGGATGTGGGCCAGCTGCCTGGTTTGACCATCTGGCAAATTGAGAACTTTGTGCCTGTTCTGGTGG
  AAGAAGCCTTCCATGGCAAGTTCTACGAGGCTGACTGCTACATTGTGCTCAAGACCTTCCTGGATGACAG
  CGGCTCCCTGAACTGGGAGATCTACTACTGGATTGGTGGGGAGGCCACACTTGACAAGAAAGCCTGTTCC
  GCCATTCATGCAGTCAATCTGCGCAACTACCTGGGTGCTGAGTGCCGTACTGTTCGAGAGGAGATGGGTG
  ACGAGAGTGAGGAGTTCCTGCAGGTGTTTGACAATGACATCTCCTACATTGAAGGCGGAACAGCCAGTGG
  CTTCTATACTGTGGAAGATACACACTATGTTACCAGGATGTACCGTGTATATGGGAAAAAGAACATCAAA
  TTGGAGCCTGTGCCCCTCAAAGGGTCCTCACTAGATCCAAGGTTTGTGTTTCTCCTGGACCAAGGGCTGG
  ACATTTATGTGTGGCGGGGGGCCCAGGCCACACTGAGTAACACGACTAAGGCCAGGCTCTTTGCAGAGAA
  AATAAACAAGAATGAGCGGAAAGGGAAGGCGGAAATCACACTCCTGGTGCAGGGCCAAGAGCCCCCAGGG
  TTCTGGGATGTGCTGGGTGGAGAGCCTTCTGAGATCAAGAATCATGTGCCTGATGACTTTTGGCCACCCC
  AGCCCAAGCTATACAAGGTGGGCCTGGGTCTGGGCTACTTGGAGCTTCCGCAGATCAACTACAAGCTCTC
  AGTGGAACACAAAAAACGGCCCAAGGTGGAGCTGATGCCAGGAATGAGGCTGCTGCAGAGCCTACTGGAC
  ACACGATGCGTGTACATCCTGGACTGCTGGTCTGATGTATTCATCTGGCTGGGCCGCAAGTCCCCACGCC
  TAGTGCGTGCAGCAGCACTCAAACTTGGCCAGGAGCTATGTGGGATGCTACACCGGCCACGACATACAGT
  GGTCAGCCGCAGCCTGGAGGGCACCGAGGCCCAGGTGTTCAAGGCCAAGTTCAAGAACTGGGACGATGTG
  TTGACAGTGGACTACACACGGAATGCAGAGGCTGTGCTTCAGGGCCAGGGCCTCTCTGGGAAGGTGAAGC
  GTGACACTGAGAAGACAGACCAGATGAAGGCTGACCTCACTGCACTCTTCCTACCTCGGCAGCCCCCCAT
  GCCCCTGGCTGAGGCAGAGCAGCTGATGGAGGAGTGGAATGAGGACCTGGATGGCATGGAGGGCTTCGTG
  CTGGAGGGCCGCAAGTTCACTCGACTGCCAGAGGAGGAGTTTGGCCACTTCTACACACAGGACTGCTATG
  TCTTCCTCTGCAGGTACTGGGTACCTGTGGAGTACGAGGAGGAAGAGAAGACAGAAGACAAGGAGGGGAA
  GGCCTCGGCAGAGGCCAGAGAAGGGGAGGAAGCAGCAGCGGAGGCAGAGGAGAAGCAGCCAGAGGAGGAC
  TTTCAGTGCATCGTTTACTTCTGGCAGGGCCGGGAGGCCTCCAACATGGGCTGGCTGACCTTCACATTCA
  GTCTTCAGAAGAAGTTTGAGAGCCTCTTCCCTGGCAAGCTGGAGGTAGTACGTATGACACAGCAGCAGGA
  GAACCCCAAGTTCCTATCCCATTTCAAAAGAAAGTTCATCATTCACCGAGGCAAGAGGAAGGTGACCCAG
  GGTACTCTTCAGCCGACCCTCTATCAGATCCGCACCAACGGCAGTGCCCTCTGCACCCGGTGCATCCAGA
  TTAACACTGACTCCAGCCTTCTCAACTCTGAGTTCTGCTTCATCCTCAAGGTCCCCTTTGAAAGCGAGGA
  CAACCAAGGCATTGTGTATGCCTGGGTAGGTCGGGCATCAGACCCCGACGAAGCCAAGCTGGCAGAGGAC
  ATCCTGAACACTATGTTTGATGCATCCTACAGCAAACAGGTCATCAATGAAGGCGAGGAGCCAGAGAACT
  TCTTCTGGGTAGGCATTGGTGCACAGAAACCCTATGATGACGATGCGGAGTACATGAAGCACACGAGGCT
  CTTCAGGTGCTCCAATGAGAAGGGTTACTTCGCAGTGACTGAGAAATGCTCTGACTTTTGCCAAGATGAC
  CTGGCAGACGATGACATCATGCTGCTAGACAATGGCCAAGAAGTCTACATGTGGGTTGGGACCCAGACAA
  GCCAAGTGGAGATCAAACTGAGTCTGAAGGCTTGCCAGGTATACATCCAGCACACACGCTCTAAAGAACA
  TGAGCGGCCACGTCGCCTGCGCCTGGTCCGCAAAGGTAATGAGCAGCGCGCCTTCACCCGCTGCTTCCAC
  GCCTGGAGCACGTTCCGCCAAGCCCCAGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302207
• Insert Size: 3813 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302207.1, NP_001289136.1
• RefSeq Size: 4068 bp
• RefSeq ORF: 3813 bp
• Locus ID: 14248
• Cytogenetics: 11 37.81 cM
• Gene Summary: This gene encodes a protein with gelsolin-like repeats and an N-terminal leucine-rich repeat domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. This protein may act as an actin-remodelling protein as well as a transcriptional coactivator. Homozygous knockout mice show embryonic lethality. This protein may act to regulate wound repair. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in isoform 2, which is shorter by one amino acid, compared to isoform 1.