Plekhg5 (NM_001285999) Mouse Untagged Clone

CAT#: MC229340

Plekhg5 (untagged) - Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 5 (Plekhg5), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plekhg5
• Synonyms: BC023181; Syx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229340 representing NM_001285999
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGGACCGGGCGTCTCCGGGCGCCGAGCAGCAGCCAGGCCCAGCTCTGAGCTACCCTCCCCAG
  ACTCCCAGCTCCTCTGGGTCGGGGGTCATGCCCACAGCAGCGACAGCCAGGTATGCCATCATGCCGACTG
  CCAGCAGCTGCACCACCGGGGGCCTCTCAACCTGTGTGAGACCTGTGATAGCAAGTTCCACAGCACCCTG
  CACTACGACGGCCATGTCCGATTTGACCTGCCCCCGCAAGGCTCTGTCCTGGCCCGGAATGTTTCCACCC
  GGTCCTGTCCTCCCCGCACCAGCCCTGCTGCAGACCTGGAGGAAGAAGAGGAAGGCTGTACCGACGGAAA
  AGGGGACCGGAAGAGCGCCGGCCTAAAAATCTCTAAGAAGAAAGCAAGGAGGAGACACACAGATGACCCG
  AGCAAGGAGTGCTTCACCTTGAAATTCGACCTGAACGTGGACATCGAAACAGAAATTGTGCCAGCCATGA
  AGAAGAAGTCACTGGGGGAAGTACTGCTGCCTGTGTTTGAGAGGAAGGGCATCGCACTGGGCAAGGTAGA
  CATCTACCTGGATCAATCCAACACACCCCTGTCCCTCACCTTCGAGGCCTACAGGTTTGGAGGACACTAC
  CTGCGGGTCAAAGCCAAGCCGGGGGACGAGGGCAAGGTGGAGCAGGGAGTGAAGGATTCCAAGTCCCTCA
  GTCTGCCAGCCCTGAGGCCCAGTGGGGCTGGGCCCCCTGTGTCGGAACGTGTGGACCCTCAGAGCCGCCG
  AGAGAGCAGTCTGGACATCTTGGCCCCTGGCCGCCGTCGCAAGAACATGTCTGAATTCCTGGGAGAGGCA
  GGCATCCCTGGACACGAGCCCCCCGCACCTTCCAGCTGTTCTCTGCCCGTTGGCAGCAGTGGGGGTACCA
  GCAGTGGCATCAATGAGAGCTGGAAGAACCGGGCAGCCAGTCGCTTCAGCGGCTTCTTCAGCTCCAGCCC
  GAGCACCAGTGCCTTTAGCCGGGAGGTAGACAAGATGGAACAGCTGGAGAGCAAGCTCCATGCCTACAGC
  CTTTTCGGGCTACCCCGGATGCCCAGGAGGCTGCGCTTTGACCATGACTCCTGGGAGGAGGAAGAGGAAG
  ATGACGAGGAGGATGAAGAGAGTTCAGGCCTGAGGCTGGAGGACAGCTGGAGGGAGCTCACTGATGGGCA
  TGAGAAGCTGACCAGGCGGCAGTGCCACCAACAGGAGGCAGTGTGGGAACTCCTCCACACAGAAGTCTCC
  TACATCCGGAAACTACGTGTGATCACCAACCTGTTCCTGTGTTGTCTTCTTAACCTACAAGAGTCGGGGC
  TCCTGTGTGAGGTTGAGGCTGAGCGCTTGTTCAGCAACATTCCTGAGATCGCCAAGCTACACCGCGGCTT
  GTGGGGCAGCGTGATGGTGCCAGTGCTGGAGAAGGCGCGGCGGACGCGGGCGCTGCTGCAGCCCAGCGAC
  TTTCTCAAAGGCTTCAAGATGTTCGGCTCACTCTTCAAGCCATACATCCGATACTGTATGGAAGAGGAGG
  GCTGCATGGAGTACATGCGCGGCCTGCTGCGTGACAACGATCTGTTCCGGGCATACGTCACGTGGGCGGA
  GAAGCACCAGCAGTGCCAGCGGCTGAAGCTGAGTGACATGCTGGCCAAGCCCCACCAGCGGCTCACCAAA
  TACCCACTGCTGCTCAAGTCGGTGCTAAGGAAGACAGATGATCCCCGCACCAAGGAAGCCATCGTCACCA
  TGATCAGCTCGGTGGAACGCTTCATACACCACGTGAACACGTGCATGCGGCAGCGGCAGGAGCGGCAGCG
  CCTGGCGGGGGTGGTGAGCCGGATCGATGCCTACGAGGTTGTGGAGGGCAGCAACGACGAGGTGGATAAG
  CTCCTGAAGGAATTTCTACATCTGGACCTGACAGCACCCATGCCTGGAACCTCCCCTGAAGAGACCCGAC
  AGCTGCTGCTGGAAGGGAGCCTGAGGATGAAGGAGGGGAAAGATAGCAAGATGGACGTGTACTGCTTCCT
  CTTCACTGACCTACTCTTGGTGACCAAGGCCGTGAAGAAAGCAGAGAGGACGAAGGTCATCAGGCCACCG
  CTACTGGTGGACAAGATTGTGTGCCGGGAACTTCGGGACCCTGGTTCCTTCCTCCTCATCTACCTGAATG
  AATTCCACAGTGCTGTGGGGGCCTACACTTTCCAGGCCAGCAGCCAGGCCTTGTGCCGGAGCTGGGTGGA
  CACTATTTACAACGCACAGAACCAGCTGCAGCAGCTGCGTGCACAGCTCAGTGCACAGGAGCACCCAGGC
  AGCCAGCATCTGCAGAGCCTGGAAGAGGAGGAAGATGAGCAGGAGGAGGAGGGTGAAGAGAGCGGTACCT
  CAGCTGCCAGCTCCCCCACCATCCTTCGAAAGAGCAGCAACAGCCTCGACTCTGAGCACTGCACCTCAGA
  TGGCTCCACGGAGACCCTGGCCATGGTTGTGGTAGAGCCTGGGGCGACACTGTCTTCCCCGGAGTTTGAA
  GGCGGTCCCGTCAGCTCCCAGTCGGACGAATCTTCTCTCAGTAACACTGCTTCCTCTGTCACTCCCACCA
  GCGAGCTGCTGCCCCTGGGCCCTGTGGATGGCCGCTCCTGCTCCATGGACTCCGCCTATGGCACCCTGTC
  CCCCACTTCCCTTCAAGACTTTGTGGCTCCACACCCCGTGGTCGAGCCAGCGCCTGTGCCTCAGACCCCC
  TCTCCCCAGCCCTCACCCCGCCTCCGCCGTCGCACTCCTGTCCAGCTGCTCCCTCGCCCGCCCCGCCTGC
  TCAAGTCCAAATCGGAGGCTAGCCTCTTACAATTACTGTCAGGAACCCCAGCCGCCCGTGGCGTGCCTCC
  GGCTCCCAGCCGCAGCCTGTCAGAACTGTGCCTGATCTCTGTGGCCCCTGGGGTTAGGACTCAGCGCCCC
  CTTCAGGAAGGTGGTCCTGGCTGGAATGGTCCAGGGATGTGTGACCCCTGCCATGGCCCTCAGTTGTCAG
  AATCTGAGAACAGACCTAGCCACATGACGGGGGGACCTGCAGATTCTGCCCGAAGGAGATGCAGAGAGAT
  GCCTTCTGGGACTATGTCCAGGGTGCAGTCTGAGCCTCCCTCAGGGGTCTCTGCTCAACACAGGAAGCTG
  ACCCTGGCCCAGCTCTACCGGATCAGGACCACCCTGCTGCTCAACTCCACGCTCACTGCCTCGGAGGTGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285999
• Insert Size: 3222 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285999.1, NP_001272928.1
• RefSeq Size: 3873 bp
• RefSeq ORF: 3222 bp
• Locus ID: 269608
• UniProt ID: Q66T02
• Cytogenetics: 4 E2
• Gene Summary: This gene encodes a protein belonging to the Rho guanine exchange factor (GEF) family of proteins, which activate GTPases by replacing GDP with GTP. This family member is a RhoA GEF that plays a role in endothelial cell migration and tube formation. It is required for angiogenesis and may function in neuronal cell differentiation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Oct 2013]
  Transcript Variant: This variant (1, also known as Syx1) represents the longer transcript and encodes the supported protein. CCDS Note: The coding region has been updated to represent an alternative splicing pattern that is more supported by the available transcript and protein data.