Sytl2 (NM_001289584) Mouse Untagged Clone

CAT#: MC229180

Sytl2 (untagged) - Mouse synaptotagmin-like 2 (Sytl2), transcript variant 7

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sytl2
• Synonyms: AI266830; mKIAA1597; Slp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229180 representing NM_001289584
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGACTTAAGTTTCCTGACAGAGGAGGAGCAAGACGCCATCTTAAAGGTTCTGCAGAGGGATGCTG
  CCCTGAAGAGGGCTGAAGAGGAGAGAGTCAGACATCTGCCTGAAAAAATTAAGGATGACCAACAACTAAA
  GAATATGAGTGGCCAATGGTTTTATGAAGCTAAGGCAAAAAGACACAGGGACAAAATCCACGGTGCTGAC
  ATCATCAGAGCATCCATGAGAAGGAAGAAGCTCCCAGCGGCAGAGATAGCTGTTGTGGAAGAGCCAGAAG
  ATGACACAGATCCCGCAGGCCCAAGTTCCAGTTTGGTGGATCCAGCTTCCAGTGTGATTGATATGTCCCA
  GGAAAGTACGAGGACTCCAGCTGTGTCTCTGCCCAAGCAAAGGAAGAATCCATTCAACAGCCCCAAGCTG
  CCGGAAGATCACTCCTTGCAACAAACCAAACCCGAGCAGTCAAAAACTGGAAAAGCTGGTTTATTTCAGA
  TTTCAAAAGAGGGTGAGTTGTCAGAATCAAAAGAAAAGTCATCTATCCCAGATATGCCACGTCAGCAGTT
  AGAGAAACCAAAGCAAACTGTATCCACAGAGCCTGAAAATGCGTCTCATACCAAAGCTCCAATCCCCAAA
  GCCAGAAAACTGATCTACAAATCAAATGACTTAGAGAAAGATGATAACCAGTCTTTTCCCAGACAACGGA
  GGGACTCCCTGAATGCGAGAGGGGCTCCAAGAGGGATCCTAAAGCGCAACTCCAGTTCCAGCAGCACGGA
  CTCAGAGACCCTCCGTTTAAATTACAACCTTGATCCGAAAAGCAAAATCCTATCACCTGGCCTAACCATC
  CACGAGAGAATCTCTGAGAAGGAATTTTCTTTAGAAGACGACTCTTCCACAAGCTCACTAGAGCCGTTAA
  AGCACGTGAGATTCTCTGCCGTGAAGAATGAGCTTCCACAAAGTCCTAGACCCGTCCTTGGCCAGGAAGT
  GGGAGAATTTACTGTTTTAGAATCTGACCAGTTGCAAAATGGAACTGAAGATGCAGGGGACATAGAGGAG
  TTTCAGAATCACCCAGAGCTCTCCCACAAAACACCTTTGTCTCATTATCAGTTAGTGTCAAGCCCAAGCG
  ATTCAGGAAGGGAAAGAGAACAGCTGATGTCTTCTGGGTCTGCTCCAAGAGATGAGATCCCTTGTCATTC
  AGACATTCTACCCACAGGACCTCAGTGTGTTGAGAGTTCATCCGTCATCAATGGGCAACAAGAGAAATCA
  TCACATTTTACAAAGCTTCCATCAGAATTGTCCAAAAGCCCTTCCGATGAACTGACTCAGTGTGGTGAGC
  CTGAGCCATCACAGACAGCAGACCACAGTTTTAGAGACCATCGGCAAGGTTCAGAAGAAGAGCACAGCCC
  TGTTTTGAAAACTCTGGAAAGAAGAGCTGCTAGGAAATTGCCTTCCAAAAGTCTAGAAGACATTCCATCA
  GATTCATCAAATCAAGCAAAAGTAGATAATCTGCCTGAAGAATTAGTGCGTAGTGCTGAAGATGATCAAA
  AAGCCGACCAAGAACCAGATACAAATGAATGTATACCAGGAATTTCCACAGTGCCTTCACTGCCTGATAA
  TCAGTTTTCCCACCCTGACAAACTCAAAAGGATGAGCAAGTCCGTGCCAGCATTTCTTCAAGATGAGAGC
  GATGACAGAGAAACAGACACAGCATCAGAGAGCAGCTACCAGCTCAGGAGATACAAGAAGAGCCCCAGCT
  CATTAACCAATCTTAGCAGCTCCTCTGGCATGACGTCCTTGTCCTCTGCGAGTGGCAGTGTGATGAGCGT
  TTACAGTGGAGACTTTGGCAACCTAGAAGTGAAAGGAAGCGTGCAGTTTGCACTCGACTACGTGGAGTCC
  CTGAAAGAGCTGCATGTGTTTGTGGCCCAGTGTAAGGATTTAGCAGCAGCAGATGTTAAGAAACAGCGCT
  CAGATCCGTATGTAAAGACCTATCTGCTACCAGACAAAGGCAAAATGGGCAAGAAGAAGACACTCGTAGT
  GAAGAAGACCTTGAATCCTGTATACAACGAGATATTGCGGTATAAAATTGAAAGGCAATTCTTAAAGACG
  CAGAAGTTGAACCTGTCCGTTTGGCATCGGGATACATTTAAGCGCAACAGCTTTCTGGGGGAGGTGGAGC
  TCGACCTGGAAACGTGGGATTGGGACAGCAAACAGAACAAACAGCTGAAGTGGTACCCACTGAAGAGGAA
  GACAGCACCAGTTGCCCTCGAGACAGAAAACAGAGGTGAAATGAAACTAGCTCTCCAGTATGTTCCGGAA
  CCAAGCCCTGGCAAAAAGCTTCCTACAACTGGAGAAGTCCACATCTGGGTGAAGGAATGCCTTGACCTCC
  CACTGTTGAGGGGCAGCCACCTAAATTCTTTTGTTAAATGTACCATCCTTCCAGATACCAGTAGAAAAAG
  TCGCCAGAAGACAAGAGCTGTAGGGAAAACCACCAACCCCGTCTTCAACCATACCATGGTGTATGATGGG
  TTCAGGCCTGAAGATCTGATGGAAGCCTGTGTAGAACTCACAGTCTGGGACCATTATAAACTAACCAACC
  AGTTTCTGGGAGGTCTCCGGATCGGCTTTGGAACAGGAAAAAGCTACGGGACTGAAGTGGATTGGATGGA
  TTCTACTTCTGAGGAAGTTGCTCTCTGGGAGAAGATGGTAAACTCTCCCAACACTTGGGTTGAAGCGACG
  CTGCCCCTCCGGATGCTTCTGATTGCCAAGCTTTCCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289584
• Insert Size: 2772 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289584.1, NP_001276513.1
• RefSeq Size: 4698 bp
• RefSeq ORF: 2772 bp
• Locus ID: 83671
• UniProt ID: Q99N50
• Cytogenetics: 7 E1
• Gene Summary: Isoform 11 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. Required for cytotoxic granule docking at the immunologic synapse. Isoform 1 may play a role in melanosome transport and vesicle trafficking. It controls melanosome distribution in the cell periphery and regulates melanocyte morphology. Isoform 1 acts as a positive mediator of mucus secretion by the surface mucus cells of the stomach. Mediates basal mucus secretion by gastric surface cells by promoting the proper granule biognesis and docking of mucus granules with the apical plasma membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region, compared to variant 6, resulting in an isoform (7, also known as Slp2-a delta 2S-I) that is shorter than isoform 6.