Ush1c (NM_001291182) Mouse Untagged Clone

CAT#: MC229057

Ush1c (untagged) - Mouse Usher syndrome 1C (Ush1c), transcript variant b2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ush1c
• Synonyms: 2010016F01Rik; harmonin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229057 representing NM_001291182
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGGAAGGTGGCCCGAGAATTCCGACACAAGGTGGATTTTCTCATTGAAAATGACGCAGAGAAGG
  ACTATCTTTATGATGTGCTGCGGATGTATCACCAGACCATGGATGTGGCTGTGCTCGTGGGAGACCTGAA
  GCTGGTCATCAATGAACCCAACCGCCTGCCGCTGTTTGATGCCATTCGACCCCTGATCCCACTGAAGCAC
  CAGGTGGAGTATGACCAGCTGACACCCCGACGCTCCAGGAAACTGAAGGAGGTACGCTTGGACCGTCTGC
  ACCCAGAAGGTCTCGGCCTCAGCGTGCGTGGAGGCCTGGAATTTGGCTGTGGACTCTTTATCTCCCACCT
  CATCAAAGGTGGCCAGGCAGACAGCGTTGGGCTTCAGGTAGGGGATGAAATTGTCCGGATCAACGGCTAT
  TCCATCTCTTCCTGTACCCATGAGGAAGTCATCAACCTGATCCGCACCAAGAAGACCGTGTCCATCAAAG
  TGAGACACATCGGACTGATCCCTGTGAAGAGCTCTCCTGAGGAGTCCCTCAAATGGCAGTATGTGGATCA
  GTTCGTGTCGGAATCTGGGGGTGTGCGAGGTGGCTTGGGCTCACCTGGCAATCGGACAACCAAGGAGAAG
  AAGGTGTTTATCAGTCTAGTGGGCTCTCGGGGCCTGGGCTGCAGCATCTCCAGTGGCCCCATCCAGAAGC
  CTGGCATCTTCGTCAGCCACGTGAAGCCTGGCTCCCTGTCTGCAGAGGTGGGGTTAGAGACAGGAGACCA
  GATTGTGGAAGTCAACGGCATAGACTTCACCAACCTGGACCACAAGGAGGCTGTGAATGTCCTGAAGAGC
  AGCCGCAGCCTGACCATCTCCATCGTTGCTGGAGCCGGCCGGGAGCTGTTCATGACGGACCGGGAACGGC
  TGGAGGAGGCACGGCAGCGTGAGCTGCAACGGCAGGAACTCCTCATGCAGAAGCGGCTGGCCATGGAGTC
  CAACAAGATCCTCCAGGAGCAGCAGGAGATGGAGCGCCAGAGGAGAAAGGAGATCGCCCAGAAGGCTGCC
  GAGGAGAATGAGAGATACCGGAAGGAGATGGAACAGATCTCGGAGGAGGAAGAGAAGTTTAAGAAGCAGT
  GGAAAGAAGACTGGGGTTCAAAGGAGCAGCTCATTCTGCCTAAGACCATCACCGCAGAGGTGCACCCGGT
  GCCCCTTCGCAAGCCCAAGTCTTTTGGGTGGTTTTACCGTTACGATGGCAAATTCCCAACCATCCGGAAG
  AAAGCAAAAGAGAAGAAGAAAGCCAAGTACGATAGCCTGCAGGACCTGAGGAAGAACAAGAAGGAACTCG
  AGTTTGAACAAAAGCTCTACAAAGAGAAGGAAGAGATGCTGGAGAAGGAGAAGCAGCTCAAGATCAACCG
  GCTGGCCCAGGAGGTGTCTGAGACTGAGCGGGAAGACCTTGAGGAATCGGAAAAGACTCAGTATTGGGTG
  GAGAGGCTCTGTCAGACGCGCCTCGAGCAGATTTCCTCTGCAGAGAATGAGATTCCTGAGATGACCACAG
  GGCCCCCACCTCCCCCACCTTCTGTGTCTCCTCTGGCCCCACCCTTGAGACGATTCGCTGGTGGGATCCA
  CCTCCACACCACTGACCTGGATGACATCCCCTTGGACATGTTCTACTACCCCCCCAAGACTCCCTCAGCA
  CTGCCCGTGATGCCCCACCCTCCATCTGTCAACCCACCTTCCAAGGTCCCCGCGCCCCCTGTGCTTCCCT
  CATCAGGTCACGTGAGCAGCTCATCCTCACCATGGGTGCAACGCACTCCTCCCCCAATCCCCATTCCTCC
  GCCTCCATCCATTCCCACCCAAGACCTCACTCCTACCCGCCCATTGCCCTCAGCACTGGAAGAGGCCCTG
  GGCAACCACCCCTTCCGCACTGGGGACCCAGGCCATCCAGCAGATGACTGGGAGGCAAACACCCACAGTG
  GGAAGCCCTCCAGCTCCCCCACCACTGAGCGGAGCTTCCCACCTGCCCCAAAGACATTCTGCCCAAGCCC
  ACAGCCTCCACGAGGCCCTGGCGTGTCCACCATCTCCAAACCCGTCATGGTCCACCAGGAACACAACTTC
  GTCTACAGGCCTGCTGTGAAGTCCGAGGTTCTGCCGCAGGAGATGCTGAAGAGGATGGTGGTATATCAGA
  CAGCATTCAGACAAGATTTTCGAAAATACGAAGAAGGCTTTGATCCCTACTCCATGTTCTCGCCAGAGCA
  GATCGCAGGGAAGGACGTCCGGCTTCTGCGAATCAAGAAGGAGGGGTCTTTAGACTTGGCCCTGGAGGGA
  GGCGTGGACTCCCCCGTGGGGAAGGTGGTCGTCTCTGCTGTTTATGAAGGGGGAGCTGCGGAGAGGCATG
  GTGGCGTTGTGAAAGGGGATGAGATCATGGCCATCAATGGCAAGATTGTGACGGACTACACCCTGGCTGA
  GGCCGAGGCTGCCCTACAGAAGGCCTGGAATCAGGGCGGGGACTGGATAGACCTTGTGGTTGCTGTCTGT
  CCCCCTAAGGAGTATGATGACGAGCTCCGAAAGCCCCCAGCTGGCCCGAAAGCAGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291182
• Insert Size: 2580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291182.1, NP_001278111.1
• RefSeq Size: 3042 bp
• RefSeq ORF: 2580 bp
• Locus ID: 72088
• Cytogenetics: 7 29.66 cM
• Gene Summary: Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles (PubMed:19447093). As part of the intermicrovillar adhesion complex/IMAC plays a role in brush border differentiation, controlling microvilli organization and length. Probably plays a central regulatory role in the assembly of the complex, recruiting CDHR2, CDHR5 and MYO7B to the microvilli tips (PubMed:24725409, PubMed:26812018).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (b2) uses an alternate splice junction at the 5' end of an exon compared to variant b3, that causes a frameshift. The resulting isoform (b2) has a shorter and distinct C-terminus compared to isoform b3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.