Nup88 (NM_001276406) Mouse Untagged Clone

CAT#: MC228839

Nup88 (untagged) - Mouse nucleoporin 88 (Nup88), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nup88
• Synonyms: Nup84; Prei2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228839 representing NM_001276406
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCCGTGGGGCCCTTGGGCGATGGGGAGCTGTGGCAGAGCTGGCTTCCTAACCACGTCGTGT
  TCTTGCGGCTCCGCGAGGGCGTAAGAAACCAGAGTCCAGCCGAAGCGGAGAAGCCAGCGGCTTCGACTTC
  ACCCTCGTGCCCGTCTCTGCCGCCGCATTTGCCGACGAGAAACCTGGTCTTCGGCCTCGGAGGGGAACTG
  TTTCTGTGGGACGCAGAAGGCAGCGCCTTCTTGGTGGTTCGCCTTCGAGGCCCCAGCGGTGGTGGCGTGG
  AGCCTCCTCTCTCCCAGTATCAGAGATTACTCTGCATTAATCCACCCTTGTTTGAAATCCATCAAGTCTT
  GTTGAGTCCAACCCAACATCATGTAGCACTTATCGGAAGTAAAGGACTTATGGCATTAGAATTACCTCAG
  AGGTGGGGGAAGGACTCTGAATTTGAAGGTGGAAAAGCAACTGTGAATTGTAGCACCATCCCGATTGCTG
  AGAGATTTTTCACCAGCTCTACCTCTCTGACTCTGAAGCATGCTGCATGGTATCCAAGTGAGATGCTGGA
  TCCCCACATAGTGCTGTTGACATCAGACAACGTGATAAGAATTTATTCTCTCCGTGAGCCCCAGACACCC
  ACTAAGGTGATTGTACTTTCAGAAGCAGAAGAGGAAAGTTTAATACTCAATAAAGGAAGAGCATATACAG
  CGTCTCTAGGAGAGACTGCAGTGGCGTTTGACTTCGGGCCCCTGGTAACCGTCTCAAAGAATATATTTGA
  ACAGAAAGACAGAGATGTGGTGGCGTATCCACTGTACATCCTGTATGAGAATGGGGAGACCTTCCTTACC
  TACGTGAGCCTGTTACACAGCCCAGGGAATATTGGGAAGCTGTTGGGCCCACTGCCTATGCATCCTGCAG
  CTGAAGATAACTACGGTTACGATGCCTGTGCTATACTCTGTTTGCCCTGTGTTCCAAATATCTTAGTAAT
  TGCGACTGAGTCAGGAATGCTGTACCACTGTGTTGTACTAGAGGGAGAAGAAGAAGATGACCAAACGTTA
  GAAAAGTCCTGGGATCCCAGGGCTGACTTCATCCCTTCTCTGTACGTGTTTGAGTGTGTTGAGTTAGAGC
  TTGCCCTGAAGCTGGCATCTGGAGAGGACGATCCCTTTGCCTCTGACTTTTCCTGCCCAATTAAACTGCA
  CAGAGATCCCAAGTGTCCTTCGAGATACCACTGCAGCCATGAAGCTGGCGTGCACAGTGTGGGGCTGACT
  TGGATTCACAAACTGCACAAATTTCTTGGATCGGATGAAGAAGATAAGGATAGTTTACAAGAACTCACTG
  CTGAGCAGAAATGCTTTGTGGAGCACATTCTTTGTACAAAGCCATTGCCGTGCAGGCAGCCAGCTCCAAT
  TCGAGGATTCTGGATCGTCCCAGACATCCTGGGGCCCACAATGATCTGCATCACCAGTACCTATGAATGT
  CTCATACGGCCTTTATTAAGTACAGTCCACCCAGCATCTCCTCCCCTGCTCTGTACCCAAGAAGATGCTG
  AAGTTGCAGAGTCTCCACTGCGCATTCTGGCTGAAACTCCAGACTCCTTTGAGAAGCATATTAAAAGAAT
  CTTGCAGCGTAGTGCTGCCAACCCAGCATTTCTCAAAAACTGTTCTGCAAGATCATCTGAAAAGGATTTG
  GCTCCCCCTCCCGAGGAGTGTCTTCAGCTTATCAGCAGAGCCACCCAGGTGTTCCGAGAACAGTACATTC
  TCAAGCAGGACCTGGCCAAGGAGGAGATTCAGCGGAGGGTCAAATTATTATGTGACCAAAAGAGGAAACA
  ACTCGAAGATCTCAATTACTGTCGAGAGGAAAGGAAAAGTCTCCGGGAAATGGCTGAGCGCTTAGCTGAC
  AAATATGAGGAAGCCAAAGAAAAACAAGAAGATATCATGAACAGGATGAAAAAAGTGCTTCACAGTTTTC
  ATGCTCAGCTCCCAGTTCTCTCTGACAGTGAGAGAGACATGAAGAAAGAGTTACAGCTGATACCTGATCA
  ACTGCGACATCTAGGCAACGCCATCAAACAGGTTACTATGAAAAAAGATTATCAACAGCGGAAGATGGAA
  AAAGTGCTGAGTCCTCAGAAACCCACCATTACTCTCAGTGCCTACCAGCGAAAGTGCATTCAGTCCATCC
  TGAAAGAAGAGGGTGAACACATAAGGGAAATGGTGAAGCAAATCAATGACATCCGAAATCATGTCACCTT
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276406
• Insert Size: 2244 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276406.1, NP_001263335.1
• RefSeq Size: 2467 bp
• RefSeq ORF: 2244 bp
• Locus ID: 19069
• UniProt ID: Q8CEC0
• Cytogenetics: 11 43.21 cM
• Gene Summary: Essential component of nuclear pore complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the coding region, compared to variant 2. The encoded isoform (3) is shorter, compared to isoform 2.