Lrch1 (NM_001252132) Mouse Untagged Clone

CAT#: MC228663

Lrch1 (untagged) - Mouse leucine-rich repeats and calponin homology (CH) domain containing 1 (Lrch1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrch1
• Synonyms: 4832412D13Rik; AI552548; AW215503; Chdc1; mKIAA1016
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228663 representing NM_001252132
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGCCGGGAAGCGAACCCCAAGCTTTCGCGCCCGCTCTCTCGGTAACCGCCCTGCACCCGCATC
  TCCACCAGCACCACCAACACCACCAACACCACCAGCACCACGGAGGCACCGGCGGCACCGGCTTCAACCT
  GCCCTTGAACCGTGGTTTGGAGCGCGCGCTGGAGGAGGCGGCCAACTCTGGGGGCTTGAACCTGAGCGCC
  AGGAAGCTGAAGGAGTTTCCCAGGACTACGGCCCCGGGACATGACCTCTCGGACACGGTTCGGGCAGATC
  TATCTAAAAACAGACTGGTGGAAGTGCCAATGGAGCTGTGCCAATTTGTATCACTGGAAATTCTTAACCT
  GTATCACAACTGTATCAGAGTCATTCCTGAGGCCATTGTTAATTTGCAGATGTTGACCCACCTAAACTTG
  AGTCGAAATCAGCTGTCTGCCCTGCCCGCCTGCCTGTGTGGTCTGCCTCTCAAAGTCTTAATCGCAAGCA
  ACAACAAGCTCGGATCACTGCCTGAAGAGATAGGTCAGCTCAAACAGTTAATGGAGTTGGATGTGAGTTG
  CAACGAGATCACGGCCTTGCCCCAGCAGATAGGCCAGCTGAAGTCTCTGCGGGAACTCAATGTCCGAAGA
  AATTACCTTAAGGTTTTACCACCAGAGCTAGTAGATCTCCCCTTGGTAAAGTTTGACTTTTCCTGCAACA
  AGGTGCTGGTGATCCCCGTCTGCTTCAGGGAGATGAAGCAGCTGCAGGTGCTCCTGCTTGAGAACAACCC
  TCTGCAGTCCCCTCCGGCGCAGATTTGCACGAAAGGAAAAGTACACATATTTAAGTATTTGAGCATACAA
  GCATGCCAGATTAAGACAAGTGACTCTCTTTACCTCCCCACCATAGAGAGGCCCCATTTACACCAACACG
  TGGAAGACAGTAAGAAGGACTCCGACTCAGGGGTTGGAAGTGACAATGGAGATAAGCGGTTATCTGCCAC
  CGAGCCCTCTGATGAGGACACGGTTAGCCTCAATGCGCCCATGTCAAACATCGTGGAAGAAGACCAGACC
  ATCAAGGAGGATGCGTGCCATCGGCTCACCCCAACTAAAGGAGAATTTCAGCCGAAGCCTTCCATTTTGG
  GGGACAGCGGCATCTCAGGACAAGAAAGAGAGCAGCTTGCTGGCCGTGCAGATGCTCGACACTCGGGACT
  TATGAACTATATTAAGGACCAGGCAGAAGACTGTGAAGAGCTATTGCGGATAGAAGAGGATGCTCACTGG
  CACATGGAGGAACTACTAAATTCATCCAAAGACCGGGAGCTGGATATAGCGATGATTGAGCAGCTGAGAG
  AGGCAGAGCTGCTTCAGGATCCCAACGGTCTAAGCGCAGATATCATAGAGAGAAGTATTTTGAACCTGTT
  CCCCATGGACTCAGGAGAAGCCTCAGAATTCCCTGATCCTTCACTCAATGGCCAGTTGCAGCTGGAGACG
  TCTCCAGACCGTGAGGTGCAAAATGATCTAATGTTACAGAGTAATGGAAGCCAGTATTCTCCCAATGAGA
  TACGAGAGAACTCCCCTTCAGTGTCTCCTACTGCAAACATCACAGCTCCGTTTGGCCTGAAGCCTCGTTC
  AGTGTTTCTAAGACCGCAGAGAAACTTGGAATCTATAGACCCACAGTTTACGATCCGGAGGAAGATGGAG
  CAGATGAGAGAGGAGAAGGAGCTGGTGGAGCAACTCCGAGAGAGCATCGAGATGAGGCTGAAGGTGACCC
  TTCATGAAGACCTGGGGGCGGCACTCATGGATGGTGTCGTCCTCTGCCACCTGGCCAACCACGTCCGTCC
  GAGATCTGTCGCCAGCATCCATGTCCCCTCACCGGCTGTTCCTAAACTCAGCATGGCCAAGTGCAGGAGG
  AACGTGGAAAACTTCTTGGAAGCGTGCAGGAAACTTGGCGTTCCCGAGGAAAAGCTCTGCCTGCCTCATC
  ACATCCTTGAAGAGAAAGGCCTGGTCAAAGTGGGCACGACTGTTCAGGCACTGTTGGATGTCACCGTCAC
  AAAAGCTCTATTCACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252132
• Insert Size: 2049 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252132.1, NP_001239061.1
• RefSeq Size: 4646 bp
• RefSeq ORF: 2049 bp
• Locus ID: 380916
• Cytogenetics: 14 D3
• Gene Summary: Acts as a negative regulator of GTPase CDC42 by sequestering CDC42-guanine exchange factor DOCK8. Probably by preventing CDC42 activation, negatively regulates CD4(+) T-cell migration in response to chemokine stimulation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.