Sgip1 (NM_001285859) Mouse Untagged Clone

CAT#: MC228597

Sgip1 (untagged) - Mouse SH3-domain GRB2-like (endophilin) interacting protein 1 (Sgip1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgip1
• Synonyms: 3110007P09Rik; gag; SGIP1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228597 representing NM_001285859
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGGACTGAAAAAACGTACAAGGAAGGCCTTTGGAATACGGAAGAAAGAAAAAGACACTGACT
  CTACAGGCTCGCCAGACCGAGATGGAATGCCCAGCCCACACGAGCCACCCTACCATAGCAAAGCAGAGTG
  TGCGCGGGAAGGAGGGAAGAAAGCTTCGAAGAAAAGCAATGGGGCACCAAATGGATTTTATGCAGAAATC
  GATTGGGAAAGATATAACTCACCTGAGCTAGATGAAGAAGGTTACAGCATCAGACCTGAGGAACCAGGCT
  CTACCAAAGGAAAGCACTTTTATTCCTCAAGTGAATCTGAAGAGGAAGAAGAGTCGCACAAGAAATTCAA
  CATCAAGATTAAACCCTTGCAATCTAAGGACGTCCTGAAGAATGCAGCGACGGTAGATGAGCTGAAGGCT
  TCAATAGGCAACATCGCACTTTCCCCTTCGCCTGTGAGGAAAAGTCCGAGGCGCAGCCCGGGTGCAATTA
  AAAGGAACTTATCCAGTGAAGAAGTTGCAAGACCCAGGCGTTCCACCCCAACTCCAGAACTTACAAGCAA
  GAAGCCTCTGGATGACACTCTGGCCCTTGCTCCCCTCTTTGGTCCACCATTAGAGTCAGCTTTTGATGAG
  CAGAAGACGGAAGTTCTTTTAGATCAGCCTGAGATATGGGGTTCAGGCCAACCGATGAACCCAAGTACGG
  AGTCGCCAGAGCTAGCAAGACCTTTCCCCACTGGAACACCTCCACCTCTGCCTCCAAAAACTGTACCAGC
  CACTCCGCCTCGAACAGGCTCCCCCTTAACAGTTGCAACAGGTGCGTCTTCTCCTGCTCGACCAGCCACC
  CCCTTAGTTCCTTGCAGCACCACTCCGCCTCCACCTCCTCCCCGGCCTCCATCCCGGCCAAAGCTACCTC
  CAGGAAAGCCTGGAGTTGGAGATGTGTCCAGACCTTTTAGCCCACCCATACACTCCTCCAGCCCTCCTCC
  GATAGCACCCTTAGCCCGGGCTGAAAGCACTTCTTCAATATCGTCAACTAACTCACTGAGCGCAGCCACC
  ACTCCCACAGTTGAGAATGAACAGCCCTCCCTCGTTTGGTTTGACAGAGGAAAGTTTTATTTGACTTTTG
  AAGGTTCCTCCCGGGGGCCCAGTCCCCTAACTATGGGGGCCCAGGACACCCTCCCGGTCGCAGCAGCTTT
  CACAGAAACTGTCAACGCCTACTTCAAAGGAGCAGATCCAAGCAAGTGCATTGTTAAGATCACGGGAGAA
  ATGGTGTTGTCCTTCCCTGCTGGTATCACCAGACACTTTGCCAACAACCCATCCCCAGCAGCTCTGACTT
  TCCGGGTGGTAAATTCCAGCAGGTTAGAGCACGTCCTGCCGAACCCCCAACTTCTCTGCTGTGATAATAC
  ACAAAATGATGCCAATACCAAGGAATTCTGGGTAAACATGCCAAATTTGATGACTCACCTAAAGAAAGTC
  TCTGAACAAAAACCCCAGGCTACATACTATAATGTGGACATGCTCAAATATCAGGTGTCTGCCCAGGGCA
  TTCAGTCCACACCTCTGAACTTGGCGGTGAACTGGCGCTGTGAGCCTGCGAGCACTGACCTGCGCATAGA
  CTATAAGTACAACACGGATGCCATGTCCACTGCAGTGGCCCTCAACAACGTGCAGTTCCTGGTCCCCATA
  GACGGAGGAGTGACCAAGCTTCAGGCTGTGCTTCCTCCAGCAGTCTGGAATGCTGAACAACAAAGAATAT
  TATGGAAGATTCCCGATATCTCCCAGAAGTCAGAAAATGGAGGCGTAGGTTCTTTACTGGCGAGATTTCA
  GTTATCCGAAGGCCCGAGCAAACCTTCCCCGCTGGTTGTGCAGTTCACGAGTGAAGGAAGTACTCTCTCT
  GGCTGTGACATTGAACTTGTTGGAGCAGGCTACCGGTTTTCACTCATTAAGAAGAGGTTTGCTGCAGGAA
  AGTACTTGGCAGATAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285859
• Insert Size: 1980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285859.1, NP_001272788.1
• RefSeq Size: 5004 bp
• RefSeq ORF: 1980 bp
• Locus ID: 73094
• UniProt ID: Q8VD37
• Cytogenetics: 4 C6
• Gene Summary: May function in clathrin-mediated endocytosis. Has both a membrane binding/tubulating activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a preference for membranes enriched in phosphatidylserine and phosphoinositides and is required for the endocytosis of the transferrin receptor. May also bind tubulin. May play a role in the regulation of energy homeostasis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in both the 5' and central coding regions, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.