Zfp12 (NM_001289590) Mouse Untagged Clone

CAT#: MC228432

Zfp12 (untagged) - Mouse zinc finger protein 12 (Zfp12), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp12
• Synonyms: C530015C18; Krox-7; Zfp-12; Znf12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228432 representing NM_001289590
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGGCTGATGGAGGAGGACCAGGGAGGTGAAGAGAACCAGTCAAGTTCAGCTGTGTTCAGCTACA
  GAAGCCGGGCTGATGCCTCTAGTAAGGCCACTGATGGAGAAACAAAGCCCTTTCCCTCACAGAAGGCCCT
  CCCTCAGTGCAACTCCTGTGAGAAGAGCCTGATGTGTGTCTCGGCGTTCATCCGAAGCGACGGAAGCTAC
  GCAAAACTGAGGCCCAATGTGTGTGCCGGGTGTGGGAAGCCCCTCCCCTGCAGCAAGCCCGAGGAAACAC
  ACCCTGGAGGTGAGTCTTACGAATTTAGTGGGGATGGAGATGAGGACCCTCTCGGTGAAGAAGGCGTGTA
  TCAGAAGGGTCACTTTTTGGAGGAGCCCTTTGAATACGTTGAGTGCCAGAAATCCTTCCCCAAGGGCACT
  GTGTTTCTTAATCACCTGGAAGAGGAGCCCTGCGACTGGAATGACGCTGAGGTCGCTTTCCTGCAGACGT
  CAGACCTCAGTGCCCACCAGGATTCTCTCATGGAAATGAAGCCCTACGAGTGCCAGCAGTGTGGGAAGTC
  CTTCTGCAAAAAGTCCAAGTTCGTCATCCATCAGAGGACTCACACAGGAGAGAAGCCCTTCAAGTGCAGT
  CAGTGTGGGAAGTCCTTCTGCCAGAAGGGCACCCTCACCGTCCACCAGAGGACGCACACCGGGGAGAAGC
  CCTACGAGTGTACCGAGTGCGGGAAGACCTTCTACCAGAAGCTGCACCTCATTCAGCACCAGAGGACCCA
  CTCGGGCGAGAAGCCCTACAAGTGTGGCTACTGCGGGAAGTCCTTCTGCCAGAAGACGCATCTCACTCAG
  CACCAGAGGACGCACTCGGGAGAGAGGCCCTATGTTTGTCACGACTGTGGCAAGACGTTCTCTCAGAAGT
  CAGCACTCAATGACCACCAGAAGATCCACACGGGCGTCAAACTCTACAAATGCAGCGAGTGTGGCAAGTG
  CTTCTGCCGCAAGTCCACGCTCACAACCCACATGAGGACGCACACGGGCGAGAAGCCGTACGAATGCAAC
  GAGTGCGGCAAGTTCTTCTCCAGGCTGTCGTACCTCACCGTGCATTACAGAACCCACTCGGGGGAGAAGC
  CCTACGAGTGTGCGGAGTGCGGGAAGTCCTTCTACCTGAACTCAGCCCTTATGAGACATCAGAGGGTGCA
  CACGGGGGAGAAGCCTTACGAGTGTAACGAGTGTGGGAAATTATTCTCGCAGCTGTCCTACCTCACCGTG
  CATCACAGAACTCACTCAGGAGTGAAGCCCTATGAGTGTAGCGAGTGTGGAAAGACCTTCTACCAGAATT
  CAGCCCTCTGTAGACACCGGAGAATTCACAGAGGGGAGAAGCCCTATGAATGCTACATCTGTGGCAAGTT
  CTTTTCTCAGATGTCTTACCTCACGATCCATCACAGAATTCACTCAGGGGAGAAGCCCTATGAGTGCCGC
  GAGTGTGGGAAGTCCTTCTGCCAGAACTCAGCCCTCAACAGACACCAGCGCACGCACACGGGAGAAAAAG
  CCTACGAGTGTTACGAGTGTGGGAAATGCTTCTCCCAGATGTCTTATCTCACTATCCATCACAGGATCCA
  CTCGGGAGAGAAGCCCTTCGAGTGTAACGAGTGTGGGAAAGCCTTCTCTCGGATGTCGTACCTCACTGTG
  CACCACAGGACCCACTCAGGAGAGAAGCCCTATGAATGTACGGAGTGCGGGAAGAAATTCTACCACAAGT
  CCGCGTTCAATAGCCATCAGAGAACTCACAGGAGAGGGAGCGGGAATGGGGTTGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289590
• Insert Size: 1809 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289590.1, NP_001276519.1
• RefSeq Size: 5346 bp
• RefSeq ORF: 1809 bp
• Locus ID: 231866
• UniProt ID: Q7TSI0
• Cytogenetics: 5 G2
• Gene Summary: Transcriptional repressor which suppresses activation protein 1 (AP-1)- and serum response element (SRE)-mediated transcriptional activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate exon in the 5' region and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.