Wac (NM_001282093) Mouse Untagged Clone

CAT#: MC228430

Wac (untagged) - Mouse WW domain containing adaptor with coiled-coil (Wac), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wac
• Synonyms: 1110067P07Rik; A230035H12Rik; AI256735; AI256776; Wwp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228430 representing NM_001282093
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGACGCCGCAGATCCTTCACCACCAAACAAAATGTTGCGGAGATCTAATAGTCCTGAAAACAAAT
  ACAGTGACAGCACAGGTCACAACAAGGCCAAAAACGTGCACACTCAGAGAGTTAGAGAAAGGGACGGTGG
  GACCAGTTACTCTCCACAAGAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCT
  AATCCAAGCAATAATCCAAGCAAAACTTCAGATGCACCTTATGATTCTGCAGATGATTGGTCTGAGCACA
  TTAGCTCATCTGGAAAAAAGTACTACTACAATTGTCGCACAGAAGTTTCACAGTGGGAGAAACCAAAAGA
  GTGGCTTGAAAGAGAACAGAGACAAAAAGAAGCAAATAAGCTGGCAGTTAATAGTTTCCCAAAAGACAGG
  GATTACAGAAGAGAGGTGATGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAATGGAAGACAAGCATT
  CCAGCGATGCCAGTAGTTTGCTCCCACAGAATATTTTGTCTCAAACAAGCAGACACAATGACAAAGACTA
  CAGACTGCCAAGAGCAGAGACTCACAGTAGCTCTACGCCAGTACAGCACCCCATCAAACCAGTGGTTCAT
  CCAACCGCTACCCCAAGCACTGTTCCTTCTAGTCCATTTACGCTTCAGTCTGATCACCAGCCAAAGAAAT
  CATTTGATGCTAATGGAGCATCTACTTTATCCAAACTGCCTACACCCACAGCTTCTCTCCCTGCACAGAA
  AACAGAGAGAAAAGAATCTGCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCT
  TCAGCCTCTGGCCTGAACCCTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTC
  CACAATCAACAATACCTCCATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGC
  CACGCTACAGCTTAATAATTCTAATGTGGACATATCCAAAATAAATGAAGTTCTTACAGCAGCTGTAACA
  CAAGCTTCCCTGCAGTCTATCATCCACAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATCACCTCAC
  TGATTTCTCAAGCTGCCCAGCTTTCTACACAAGCCCAGCCATCTAATCAGTCTCCAATGTCTCTGACATC
  TGATGCTTCATCCCCAAGATCCTATGTGTCTCCAAGAATAAGCACACCTCAAACTAATACAGTCCCTATG
  AAACCTTTGATTAGTACTCCACCTGTTTCATCCCAGCCAAAGGTTAGTACTCCAGTAGTTAAGCAAGGAC
  CAGTGTCACATTCGGCTACACAGCAGCCTGTAACTGCTGACAAGCAGCAGAGCCATGATCCTGTTTCTCC
  TCGAAGTCTTCAGCGCTTAAGTAGCCAGAGAAGTCCATCACCTGGTCCAAATCATACTTGTAGTAGTAAT
  GCATCAACTGCAACTGTTGTACCACAGAATGCATCTGCTAGACCTGCGTGCTCATTAACACCTACTCTAG
  CAGCGCACTTCAATGATAATCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAGGC
  ATCAAGATTGCGTGAAGAAGCCCATAATATGGGAAGTGTACATATGTCAGAAATATGTACTGAGTTAAAA
  AATTTGAGATCATTAGTTCGAGTATGTGAAATTCAAGCAACTTTGCGAGAGCAGAGGATACTATTTTTGA
  GACAACAAATTAAGGAACTCGAAAAGCTGAAAAATCAGAATTCCTTCATGGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282093
• Insert Size: 1806 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282093.1, NP_001269022.1
• RefSeq Size: 5058 bp
• RefSeq ORF: 1806 bp
• Locus ID: 225131
• UniProt ID: Q924H7
• Cytogenetics: 18 A1
• Gene Summary: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate 5' structure, and thus differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from alignments.