Gm5141 (NM_001256065) Mouse Untagged Clone

CAT#: MC228369

Gm5141 (untagged) - Mouse predicted gene 5141 (Gm5141)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gm5141
• Synonyms: 380850
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228369 representing NM_001256065
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCAGTGACTTATGAGGACGTGCATGTGAACTTCACTCAAGAGGAGTGGGCTCTGCTGGATCCTT
  CTCAGAAGACACTCTACAAAGATGTCATGCTGGAGACCTACAGGAACCTCAGTGCTTTAGGCTTTAGTTG
  GGAAGCCCAAAATATTGAAGAACATTGTCAAAGTTCTAGAAGACACAGAAGGTGTGAAAGGAGTCAGAGT
  GCAGAGAAACCCTCTGAATATACTCAACATGGTAAAGCATTTGCTTTCCATGCTCACAGTCATGCCCAAA
  GGCATGAAAGGATTCATACAGAAAAGATCCCCTCTGAAGTTATTCACTGCATCGAAGACTTTTTACCTTA
  CACAAGTCTCCAAGTACATAAAAGAGCACAAACTGGCCAGAAACAGTATGAATATAAACAATGTGGTAAA
  GGATTTGCATGTCCCAGTCTTCTTAAAAGGCATGAAAGAATTCATACTGTCGAGAAACCCTACAAATGTA
  ATCAGTGTGATAAAGCCTTTTCCCTTAAATATAGTCTCCAAAGACATATAAGGACACATACTGGAGAAAA
  ACCCTTCAAATGTAATCAGTGTGAAAAAGCCTTTTCACATCATTGTAATCTTCAAACTCATAGAAGAACA
  CATACCAGAGAGAAACCCTTCAAATGTAATGAATGTGACAAAGCCTTTTCCCAATTTGGTAATCTCCAAA
  ATCATAGAAGGACACATACTGGAGAGAAACCCTTCAAATGTAATGAATGTGACAAAGCCTTTTCAGAGAA
  AGGTAGTCTCCAAAAACATATAAGTATACATACTGGAGAGAAACCCTACAAATGTAATCAGTGTGATAAA
  GCCTTTTCCCATAAACAGAGTCTCCTAATACATATAAGGATACATACTGGAGAAAAACCCTACAAATGTA
  ATCAGTGTGATAAAGCTTTTTCCCATAAACATATTCTCAAAAACCATATAAGGATACATAGTGGAGAAAA
  ACCCTACAAATGTAATCTTTGTGATAAAGTCTTTTCACGACACTTTCACCTCCAAAATCATATAAGAACA
  CATACTGGAGAAAAGCCTTACAGATGTAATCAATGTGATAAAGCCTTTTCACATCACAAAACTCTTCAAA
  CTCATAGAAAAACACATACCGGAGAGAAACCCTTCAAATGTAATGAATGTGACAAAGCCTTTTCAGAGAA
  AGGTAGTCTCCAATCACATATAAGGACACATACTGGAGAAAAACCCTACAAATGTAATCAATGTGATAAA
  GCCTTTTCCCATAAACAGAATCTCCAAATACATATAAGGATACATACTGGAGAAAAACCCTACAAATGTA
  ATCAATGTGATAAAGCCTTTTCCTATAAACAGAATCTCCAAATACATATAAGGATACATACTGGAGAAAA
  ACCCTACAAATGTAATCAATGTGATAAAGCCTTTTTACACCAAGGTAATCTTCAAACTCATAGAAGAACA
  CATACCAGAGACAAACCCTTCCAATGTAATCTTTGTGATAAAGTCTTTTCACGACACTTTCACCTCCAAA
  ATCATATAAGAACACATACTAGAGAGAAGCCTTACAAATGTAATGAATGTGGTAAAGGCTTTTCACAACA
  CAGTTCTCTTCAAACTCATAGAAGAACACATACCGGAGAGAAACCCTTCAAATGTAATCAGTGTGATAAA
  GCCTTTTCCTGTAAACATCATCTCCAAACACATATAAGGATACATACTGTAGAAAAACCCTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256065
• Insert Size: 1746 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256065.1, NP_001242994.1
• RefSeq Size: 3292 bp
• RefSeq ORF: 1746 bp
• Locus ID: 380850
• Cytogenetics: 13