Kyat3 (NM_001293560) Mouse Untagged Clone

CAT#: MC227784

Ccbl2 (untagged) - Mouse cysteine conjugate-beta lyase 2 (Ccbl2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kyat3
• Synonyms: Ccbl2; Kat3; KATIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227784 representing NM_001293560
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTGGCCCAGAGGAGACTCATCTCCCTTGGCTGCAGATCAAAGCCCATAAAGACAATTTACTCTT
  CTTCGAAAGTCCTCGGACTCTGCACTTCTGCCAAAATGGCTTTGAAATTCAAAAACGCCAAACGAATCGA
  AGGCCTGGACAGCAATGTGTGGGTTGAATTTACTAAGTTGGCTGCGGATCCTTCTGTGGTGAACCTTGGA
  CAAGGCTTTCCAGATATATCCCCTCCTTCATACGTAAAAGAAGAGTTATCAAAGGCTGCATTTATTGATA
  ACATGAATCAATACACAAGAGGCTTTGGTCATCCAGCACTTGTGAAAGCTCTGTCCTGCTTATATGGAAA
  GATTTATCAACGTCAAATTGATCCAAACGAAGAAATCCTTGTGGCCGTGGGGGCATATGGATCTCTCTTT
  AACTCCATCCAAGGATTGGTGGACCCAGGAGATGAAGTGATAATTATGGTGCCTTTTTACGACTGTTATG
  AGCCCATGGTGAGAATGGCCGGAGCAGTGCCTGTGTTTATTCCCCTGAGATCTAAACCTACTGACGGGAT
  GAAGTGGACTAGCTCTGACTGGACATTCGATCCTCGAGAACTGGAAAGTAAATTCAGTTCCAAAACGAAA
  GCCATAATATTAAATACTCCACACAACCCCCTCGGCAAGGTGTATACCAGACAGGAGCTGCAAGTCATTG
  CTGACCTTTGCGTCAAGCACGACACTCTGTGCATCAGCGATGAGGTTTATGAATGGCTTGTCTATACTGG
  ACATACGCACGTAAAAATAGCCACTCTTCCAGGTATGTGGGAGAGAACAATAACAATAGGAAGTGCTGGC
  AAGACATTCAGTGTGACTGGCTGGAAGCTCGGCTGGAGCATTGGCCCTGCTCACCTGATAAAGCATTTAC
  AGACCGTTCAACAGAACAGTTTTTACACGTGTGCGACTCCTTTACAGGCAGCCTTGGCCGAGGCGTTTTG
  GATCGATATCAAGCGCATGGATGACCCTGAGTGTTACTTTAATTCTCTGCCAAAGGAATTAGAAGTAAAG
  AGAGATCGGATGGTCCGTTTACTTAACAGCGTTGGCCTGAAACCCATTGTTCCTGACGGGGGTTACTTCA
  TCATTGCTGATGTGTCTTCATTAGGTGCTGACCTCTCGGACATGAACAGCGATGAGCCTTATGACTATAA
  GTTTGTGAAGTGGATGACGAAACATAAGAAACTGACAGCCATTCCTGTTTCTGCCTTCTGCGACTCCAAG
  TCTAAACCACACTTTGAGAAGCTGGTGCGGTTTTGCTTTATTAAAAAAGACAGCACACTGGATGCTGCCG
  AAGAAATCTTCAGGGCCTGGAACAGCCAGAAGTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293560
• Insert Size: 1368 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293560.1, NP_001280489.1
• RefSeq Size: 2275 bp
• RefSeq ORF: 1368 bp
• Locus ID: 229905
• UniProt ID: Q71RI9
• Cytogenetics: 3 H1
• Gene Summary: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). May catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (By similarity). Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).