Eva1c (NM_001199210) Mouse Untagged Clone

CAT#: MC227714

Eva1c (untagged) - Mouse eva-1 homolog C (C. elegans) (Eva1c), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eva1c
• Synonyms: 1700092M14Rik; 4931408A02Rik; Fam176c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227714 representing NM_001199210
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGCCAGGACACCCGCGTCCACCACCCGCGCCCCAATCGGCGCAGAATCAGGGCCTCCGCAGGC
  AGGTAGAGCCTCCGGGGCAGCTTCTGCGCCTGTTCTACTGCACGGTCCTAGTGTGCTCCAAAGAGACCTC
  CGCGCTCACCGATTTCTCTGGTTACTTAACCAAACTCCTGCAAAACCACACTGCCTATGCCTGTGACGGG
  GACTATCTGAATCTACAGTGCCCTCGGCATTCAACAATAAGTGTCCAGTCGGCATTTTATGGGCAAGATT
  ACCAAATGTGTAGTTCCCAGGAGCCCATCTCCCAGAGGGAAGACAACCTGACCTGTGTGGCATCCACCAC
  TTTGCAGAAGGTGCTGGACGAGTGCCAGAACCAGCGGGCCTGCCACCTCCTGGTCAATAGCCGAGTCTTC
  GGACCTGACCTTTGTCCAGGAAGCAGTAAATACCTCCTGGTCTCCTTTAAATGCCAACCTAATGAGTTGA
  AAAACAAGACAGTGTGTGAAAACCAGGAGTTGAAGCTACACTGCCATGAGTCCAAGTTCCTCAACATCTA
  CTCCGCCGCCTACGGCCGGAGGACCCAGCAAAGAGACATCTGCTCCTCCGGGGCGGAGCTGCTGCCCCCC
  TTCGACTGCCTGTCTTACACGGCTTTACAAGTCCTGTCCAGGAGGTGCTATGGGAAGCAGAGATGTAAGG
  TCCTCGTCGATAATTACCACTTCGGAAGCCCCTGTCTTCCAGGAGTGAAAAAATACCTCACTGTTGCCTA
  CGCATGTGTTCCCAAGAACATACTCACGGCAGTGGATCCGGCTGTTGCTAATCTAAATCCTTCTCTGAAG
  AAAGATGATGAACACGGTATAACATTCAACCCAAGCGGGTCGAGGGTTGTGCGGAAAGATGGCGTTATCG
  TCAGCAACTCTCTGGCCGCGTTCGCTTACATTCGAGCCCACCCAGAGAGAGCCGCTCTGCTGTTCATGTC
  CAGTGTCTGCATCGGCCTGCTCCTCACACTGTGTGCCCTGGTCATCAGGGTGTCCTGTACGAAGGACTTC
  CGGGAGCTGAGGCAGGGGAGGGAGCACCTGGTGCTAGGGAGCGACAAGGCGGAGGAGGACAGCGAGGAAG
  ACTTGGAAGAGGAGGACTCCTCGGATTCGCAGTTTCCCGAGGAGTTGTCCAGGTTTTGTAGGACTTCATA
  TCCAGCCTATAGCTCTATAGAAGCCGCCGAGCTGGCGGAAAGGATTGAACGCAGGGAGCAAGTCATTCAA
  GAGATATGGATGAACAGTGGCCTGGATTCCTCACTCCCCAGGAACGTCGGCCACTTCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199210
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199210.2, NP_001186139.1
• RefSeq Size: 2366 bp
• RefSeq ORF: 1323 bp
• Locus ID: 70967
• UniProt ID: P58659
• Cytogenetics: 16 C3.3
• Gene Summary: Binds heparin.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).