Sec1 (NM_001271578) Mouse Untagged Clone

CAT#: MC227445

Sec1 (untagged) - Mouse secretory blood group 1 (Sec1)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sec1
• Synonyms: Fut3; Fut10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227445 representing NM_001271578
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCGAGACTGCGTGGGACAGGAGCACTGTCGCCCCCAGCAGGCTTGAAGCTTGGTGGCCCCAAA
  CAAGCCGGCCTAGAAGGATCCAAGCAGTGCTCCAGCGACTCAGGGCCATCTGCCCACCCCTCTCCACCTT
  CTACCTCTTCTTCGTGATTTTCGTGGTGTCTACCATCTTCCACTGCCACCGGCGCCTGAGTCTGGTGCCT
  GGCCCCTGGGCCTCGCCATCCCTTGTGGTCTTCCCCCCAAGACACATGCCCCGGGAGGGCATGTTCACCA
  TCAGAGTCAAAGGCCGCCTGGGGAACCAGATGGGCGAATATGCCACGCTGTTTGCACTGGCCCGGATGAA
  TGGACGGCTTGCCTTCATCCCTGCGTCCATGCACAGCACACTAGCGCCCATCTTCAGGATCAGCCTCCCG
  GTGTTGCATAGCGACACAGCCAAAAGGATCCCGTGGCAGAATTACCACCTCAACGACTGGATGGAGGAGC
  GTTACCGCCACATCCCGGGGCAGTATGTGCGTTTCACGGGATACCCGTGCTCCTGGACCTTCTACCACCA
  CCTGCGCCCAGAGATCCTGAAGGAGTTCACCCTGCACGACCATGTGCGTGAGGAGGCCCAGGCCTTCCTG
  CGTGGCCTGCAGGTGAATGGGAGCCAGCCAAGTACCTTTGTGGGTGTCCATGTGCGCCGAGGGGACTATG
  TGCGTGTCATGCCCAAGGTGTGGAAGGGTGTGGTGGCTGACCGGGGTTACCTAGAAAAGGCCCTGGACAG
  GTTCCGGGCACGCTATTCATCTCCAGTCTTCGTGGTCACCAGTGATGACATGGCCTGGTGCCGGAAGAGC
  ATCACTGCCTCCCGAGGGGACGTGGCATTTGCTGGCAATGGCCTTCAGGGATCGCCTGCCAAGGACATTG
  CATTGCTCATGCAGTGCAACCACACCGTCATCACGCTGGGGACCTTTGGGATCTGGGCTGCCTACCTCAC
  GGGTGGGGACACTGTTTACCTGGCTAACTTTACCCAGCCCAACTCCCCCTTCCACACGGTTTTCAAGCCA
  GAAGCAGCTTACCTGCCCGAGTGGGTGGGCATCGCAGCTGACCTGGGACAGCCAAACACAGTAGGCTCTG
  GCCATGCCTCAGCCAGAGCCCCCAAGAGGCACTGGGGGGCCTTGCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271578
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271578.1, NP_001258507.1
• RefSeq Size: 2470 bp
• RefSeq ORF: 1170 bp
• Locus ID: 56546
• Cytogenetics: 7 29.43 cM
• Gene Summary: This gene is one of three genes in mouse which encode a galactoside 2-L-fucosyltransferase. These genes differ in their developmental- and tissue-specific expression. This gene is predicted to encode a type II membrane protein, which is anchored in the Golgi apparatus and controls the final step in the creation of alpha (1,2) fucosylated carbhohydrates by the addition of a terminal fucose in an alpha (1,2) linkage. The biological function of the fucosylated carbhohydrate products is thought to involve cell-adhesion and interactions with microorganisms. [provided by RefSeq, Dec 2012]